##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspI AviII AvrII BbeI
BcgI BciVI BglI BlnI BlpI
Bpu1102I BsaHI BsiEI BsiWI BspDI
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
FseI FspI Hin1I KasI KpnI
KspI MfeI MluI MslI MunI
MvnI NarI NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
Psp1406I PvuI RsrII SacII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1485 GTCGAC GT^[AC][GT]AC
AflII 837 CTTAAG C^TTAAG
AflIII 3319 ACATGT A^C[GA][CT]GT
AgeI 3456 ACCGGT A^CCGGT
AhdI 984 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1577 ATTAAT AT^TAAT
AsnI 1577 ATTAAT AT^TAAT
Asp700I 824 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 984 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1229 CACGTG CAC^GTG
BclI 3130 TGATCA T^GATCA
BfrI 837 CTTAAG C^TTAAG
BsaBI 1995 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3939 TCATGA T^CATGA
BspLU11I 3319 ACATGT A^CATGT
DraI 4108 TTTAAA TTT^AAA
DraIII 255 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 984 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4055 GAATTC G^AATTC
FauI 3062 GCGGG CCCGC(4/6)
FbaI 3130 TGATCA T^GATCA
HaeII 432 GGCGCT [GA]GCGC^[CT]
HgaI 4044 GACGC GACGC(5/10)
HindIII 3957 AAGCTT A^AGCTT
HpaI 1839 GTTAAC GTT^AAC
MamI 1995 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 763 GCCGGC GCC^GGC
NcoI 1102 CCATGG C^CATGG
NdeI 3838 CATATG CA^TATG
NgoMIV 763 GCCGGC G^CCGGC
PflMI 2652 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3456 ACCGGT A^CCGGT
PmaCI 1229 CACGTG CAC^GTG
PmlI 1229 CACGTG CAC^GTG
PshAI 3289 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1577 ATTAAT AT^TAAT
PvuII 2832 CAGCTG CAG^CTG
RcaI 3939 TCATGA T^CATGA
SacI 1638 GAGCTC GAGCT^C
SalI 1485 GTCGAC G^TCGAC
ScaI 1070 AGTACT AGT^ACT
SexAI 3442 ACCTGGT A^CC[AT]GGT
StuI 2319 AGGCCT AGG^CCT
Van91I 2652 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 824 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 856 TCCGGA T^CCGGA
1275 TCCGGA
AcsI 1406 AAATTT [GA]^AATT[CT]
4055 GAATTC
AlwNI 243 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
430 CAGGCGCTG
ApaI 2385 GGGCCC GGGCC^C
2426 GGGCCC
ApoI 1406 AAATTT [GA]^AATT[CT]
4055 GAATTC
BamHI 853 GGATCC G^GATCC
898 GGATCC
BanI 177 GGCACC G^G[CT][GA]CC
2277 GGCACC
BbvCI 1522 CCTCAGC CCTCAGC(-5/-2)
978 GCTGAGG
BsaAI 1229 CACGTG [CT]AC^GT[GA]
1947 CACGTA
BsaI 292 GAGACC GGTCTC(1/5)
2562 GAGACC
BseAI 856 TCCGGA T^CCGGA
1275 TCCGGA
BsmBI 354 GAGACG CGTCTC(1/5)
872 GAGACG
BsmI 974 GAATGC GAATGC(1/-1)
1312 GCATTC
BspEI 856 TCCGGA T^CCGGA
1275 TCCGGA
BsrFI 763 GCCGGC [GA]^CCGG[CT]
3456 ACCGGT
BssSI 3779 CTCGTG CACGAG(-5/-1)
3848 CTCGTG
BstEII 2757 GGTGACC G^GT[ACGT]ACC
3430 GGTAACC
BstPI 2757 GGTGACC G^GT[ACGT]ACC
3430 GGTAACC
Cfr10I 763 GCCGGC [GA]^CCGG[CT]
3456 ACCGGT
EcoO65I 2757 GGTGACC G^GT[ACGT]ACC
3430 GGTAACC
EcoRV 1004 GATATC GAT^ATC
1064 GATATC
MroI 856 TCCGGA T^CCGGA
1275 TCCGGA
PstI 543 CTGCAG CTGCA^G
3822 CTGCAG
SphI 781 GCATGC GCATG^C
4033 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII