################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AclI AflII AgeI AscI Asp700I AspI AviII BbeI BbrPI BcgI BciVI BclI BfrI BsaAI Bsp1407I BspLU11I BsrGI Bst1107I BstAPI BstZ17I CpoI EcoNI EcoRV FbaI FseI FspI HpaI KasI KspI MfeI MluI MunI NarI NcoI NdeI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I RsrII SacII SalI SapI SexAI SfiI SfoI SnaBI SspBI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 488 GACGTC GACGT^C AccI 2771 GTCTAC GT^[AC][GT]AC AccIII 1354 TCCGGA T^CCGGA AflIII 591 ACGTGT A^C[GA][CT]GT AhdI 2548 GACTCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2065 GTGCAC G^TGCAC ApaI 4212 GGGCCC GGGCC^C ApaLI 2065 GTGCAC G^TGCAC AspEI 2548 GACTCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3551 CCTAGG C^CTAGG BamHI 2961 GGATCC G^GATCC BbvCI 1641 GCTGAGG CCTCAGC(-5/-2) BglII 3739 AGATCT A^GATCT BlnI 3551 CCTAGG C^CTAGG BsaBI 929 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1354 TCCGGA [AT]^CCGG[AT] BseAI 1354 TCCGGA T^CCGGA BsgI 200 CTGCAC GTGCAG(16/14) BsiWI 904 CGTACG C^GTACG BspDI 270 ATCGAT AT^CGAT BspEI 1354 TCCGGA T^CCGGA BspHI 1324 TCATGA T^CATGA BsrBI 630 GAGCGG CCGCTC(-3/-3) BssHII 15 GCGCGC G^CGCGC BssSI 465 CACGAG CACGAG(-5/-1) BstBI 1056 TTCGAA TT^CGAA BstEII 3647 GGTTACC G^GT[ACGT]ACC BstPI 3647 GGTTACC G^GT[ACGT]ACC BstXI 2699 CCATCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 270 ATCGAT AT^CGAT DraIII 2427 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3217 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 970 CGGCCG C^GGCCG Eam1105I 2548 GACTCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 970 CGGCCG C^GGCCG Eco52I 970 CGGCCG C^GGCCG EcoO65I 3647 GGTTACC G^GT[ACGT]ACC EcoRI 1053 GAATTC G^AATTC HindIII 1188 AAGCTT A^AGCTT MamI 929 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1354 TCCGGA T^CCGGA NspV 1056 TTCGAA TT^CGAA PvuI 160 CGATCG CGAT^CG RcaI 1324 TCATGA T^CATGA SbfI 2180 CCTGCAGG CCTGCA^GG SfuI 1056 TTCGAA TT^CGAA SgrAI 164 CGCCGGCG C[GA]^CCGG[CT]G SmaI 2095 CCCGGG CCC^GGG SpeI 2836 ACTAGT A^CTAGT SphI 4199 GCATGC GCATG^C Sse8387I 2180 CCTGCAGG CCTGCA^GG XbaI 2645 TCTAGA T^CTAGA XmaCI 2095 CCCGGG C^CCGGG XmaI 2095 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 488 GACGTC G[GA]^CG[CT]C 3166 GGCGTC AlwNI 525 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2577 CAGAGGCTG AocI 2589 CCTCAGG CC^T[ACGT]AGG 3264 CCTCAGG Aor51HI 2785 AGCGCT AGC^GCT 3596 AGCGCT AseI 3746 ATTAAT AT^TAAT 4244 ATTAAT AsnI 3746 ATTAAT AT^TAAT 4244 ATTAAT BsaHI 488 GACGTC G[GA]^CG[CT]C 3166 GGCGTC BsaI 1549 GAGACC GGTCTC(1/5) 3590 GGTCTC BsmI 3983 GAATGC GAATGC(1/-1) 133 GCATTC BsrDI 806 GCAATG GCAATG(2/0) 4174 GCAATG Bsu36I 2589 CCTCAGG CC^T[ACGT]AGG 3264 CCTCAGG DraI 3821 TTTAAA TTT^AAA 4438 TTTAAA EarI 387 GAAGAG CTCTTC(1/4) 1944 GAAGAG Eco47III 2785 AGCGCT AGC^GCT 3596 AGCGCT Eco81I 2589 CCTCAGG CC^T[ACGT]AGG 3264 CCTCAGG EcoT22I 3316 ATGCAT ATGCA^T 3750 ATGCAT HaeII 2785 AGCGCT [GA]GCGC^[CT] 3596 AGCGCT Hin1I 488 GACGTC G[GA]^CG[CT]C 3166 GGCGTC HincII 1455 GTTGAC GT[CT]^[GA]AC 2441 GTCAAC HindII 1455 GTTGAC GT[CT]^[GA]AC 2441 GTCAAC Ksp632I 387 GAAGAG CTCTTC(1/4) 1944 GAAGAG NaeI 165 GCCGGC GCC^GGC 1684 GCCGGC NgoMIV 165 GCCGGC G^CCGGC 1684 GCCGGC NsiI 3316 ATGCAT ATGCA^T 3750 ATGCAT PshBI 3746 ATTAAT AT^TAAT 4244 ATTAAT ScaI 664 AGTACT AGT^ACT 3100 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### Acc65I AccII AciI AcsI AfaI AluI AlwI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII