##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AflII AgeI AscI Asp700I
AspI AviII BbeI BbrPI BcgI
BciVI BclI BfrI BsaAI Bsp1407I
BspLU11I BsrGI Bst1107I BstAPI BstZ17I
CpoI EcoNI EcoRV FbaI FseI
FspI HpaI KasI KspI MfeI
MluI MunI NarI NcoI NdeI
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacII SalI
SapI SexAI SfiI SfoI SnaBI
SspBI SspI SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 488 GACGTC GACGT^C
AccI 2771 GTCTAC GT^[AC][GT]AC
AccIII 1354 TCCGGA T^CCGGA
AflIII 591 ACGTGT A^C[GA][CT]GT
AhdI 2548 GACTCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2065 GTGCAC G^TGCAC
ApaI 4212 GGGCCC GGGCC^C
ApaLI 2065 GTGCAC G^TGCAC
AspEI 2548 GACTCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3551 CCTAGG C^CTAGG
BamHI 2961 GGATCC G^GATCC
BbvCI 1641 GCTGAGG CCTCAGC(-5/-2)
BglII 3739 AGATCT A^GATCT
BlnI 3551 CCTAGG C^CTAGG
BsaBI 929 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1354 TCCGGA [AT]^CCGG[AT]
BseAI 1354 TCCGGA T^CCGGA
BsgI 200 CTGCAC GTGCAG(16/14)
BsiWI 904 CGTACG C^GTACG
BspDI 270 ATCGAT AT^CGAT
BspEI 1354 TCCGGA T^CCGGA
BspHI 1324 TCATGA T^CATGA
BsrBI 630 GAGCGG CCGCTC(-3/-3)
BssHII 15 GCGCGC G^CGCGC
BssSI 465 CACGAG CACGAG(-5/-1)
BstBI 1056 TTCGAA TT^CGAA
BstEII 3647 GGTTACC G^GT[ACGT]ACC
BstPI 3647 GGTTACC G^GT[ACGT]ACC
BstXI 2699 CCATCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 270 ATCGAT AT^CGAT
DraIII 2427 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3217 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 970 CGGCCG C^GGCCG
Eam1105I 2548 GACTCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 970 CGGCCG C^GGCCG
Eco52I 970 CGGCCG C^GGCCG
EcoO65I 3647 GGTTACC G^GT[ACGT]ACC
EcoRI 1053 GAATTC G^AATTC
HindIII 1188 AAGCTT A^AGCTT
MamI 929 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1354 TCCGGA T^CCGGA
NspV 1056 TTCGAA TT^CGAA
PvuI 160 CGATCG CGAT^CG
RcaI 1324 TCATGA T^CATGA
SbfI 2180 CCTGCAGG CCTGCA^GG
SfuI 1056 TTCGAA TT^CGAA
SgrAI 164 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2095 CCCGGG CCC^GGG
SpeI 2836 ACTAGT A^CTAGT
SphI 4199 GCATGC GCATG^C
Sse8387I 2180 CCTGCAGG CCTGCA^GG
XbaI 2645 TCTAGA T^CTAGA
XmaCI 2095 CCCGGG C^CCGGG
XmaI 2095 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 488 GACGTC G[GA]^CG[CT]C
3166 GGCGTC
AlwNI 525 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2577 CAGAGGCTG
AocI 2589 CCTCAGG CC^T[ACGT]AGG
3264 CCTCAGG
Aor51HI 2785 AGCGCT AGC^GCT
3596 AGCGCT
AseI 3746 ATTAAT AT^TAAT
4244 ATTAAT
AsnI 3746 ATTAAT AT^TAAT
4244 ATTAAT
BsaHI 488 GACGTC G[GA]^CG[CT]C
3166 GGCGTC
BsaI 1549 GAGACC GGTCTC(1/5)
3590 GGTCTC
BsmI 3983 GAATGC GAATGC(1/-1)
133 GCATTC
BsrDI 806 GCAATG GCAATG(2/0)
4174 GCAATG
Bsu36I 2589 CCTCAGG CC^T[ACGT]AGG
3264 CCTCAGG
DraI 3821 TTTAAA TTT^AAA
4438 TTTAAA
EarI 387 GAAGAG CTCTTC(1/4)
1944 GAAGAG
Eco47III 2785 AGCGCT AGC^GCT
3596 AGCGCT
Eco81I 2589 CCTCAGG CC^T[ACGT]AGG
3264 CCTCAGG
EcoT22I 3316 ATGCAT ATGCA^T
3750 ATGCAT
HaeII 2785 AGCGCT [GA]GCGC^[CT]
3596 AGCGCT
Hin1I 488 GACGTC G[GA]^CG[CT]C
3166 GGCGTC
HincII 1455 GTTGAC GT[CT]^[GA]AC
2441 GTCAAC
HindII 1455 GTTGAC GT[CT]^[GA]AC
2441 GTCAAC
Ksp632I 387 GAAGAG CTCTTC(1/4)
1944 GAAGAG
NaeI 165 GCCGGC GCC^GGC
1684 GCCGGC
NgoMIV 165 GCCGGC G^CCGGC
1684 GCCGGC
NsiI 3316 ATGCAT ATGCA^T
3750 ATGCAT
PshBI 3746 ATTAAT AT^TAAT
4244 ATTAAT
ScaI 664 AGTACT AGT^ACT
3100 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI ApoI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII