################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## Acc65I AccII AccIII AclI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BlnI BlpI Bpu1102I BseAI BsiWI BspEI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII CpoI DrdI EagI Eam1105I EclXI Eco52I FseI FspI KpnI KspI MluI MroI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PflMI PmeI PshBI Psp1406I RsrII SacII SexAI SfiI SfuI SwaI Van91I ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 2871 GACGTC GACGT^C AflII 3885 CTTAAG C^TTAAG AgeI 1893 ACCGGT A^CCGGT AocI 5257 CCTGAGG CC^T[ACGT]AGG ApaI 4440 GGGCCC GGGCC^C AspI 2190 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 206 GGCGCC GGCGC^C BbrPI 3655 CACGTG CAC^GTG BciVI 2611 GGATAC GTATCC(6/5) BclI 1775 TGATCA T^GATCA BfrI 3885 CTTAAG C^TTAAG BsiEI 1598 CGATCG CG[GA][CT]^CG BspDI 1596 ATCGAT AT^CGAT BspHI 511 TCATGA T^CATGA BspLU11I 5617 ACATGT A^CATGT BstAPI 432 GCAAAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 4083 GGTTACC G^GT[ACGT]ACC BstPI 4083 GGTTACC G^GT[ACGT]ACC BstXI 5934 CCACCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 5257 CCTGAGG CC^T[ACGT]AGG ClaI 1596 ATCGAT AT^CGAT Eco81I 5257 CCTGAGG CC^T[ACGT]AGG EcoO65I 4083 GGTTACC G^GT[ACGT]ACC EcoT22I 2858 ATGCAT ATGCA^T FbaI 1775 TGATCA T^GATCA HgaI 1258 GCGTC GACGC(5/10) HpaI 615 GTTAAC GTT^AAC KasI 206 GGCGCC G^GCGCC MfeI 5478 CAATTG C^AATTG MunI 5478 CAATTG C^AATTG NarI 206 GGCGCC GG^CGCC NsiI 2858 ATGCAT ATGCA^T PflFI 2190 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1893 ACCGGT A^CCGGT PmaCI 3655 CACGTG CAC^GTG PmlI 3655 CACGTG CAC^GTG PvuI 1598 CGATCG CGAT^CG RcaI 511 TCATGA T^CATGA SbfI 5896 CCTGCAGG CCTGCA^GG ScaI 1793 AGTACT AGT^ACT SfoI 206 GGCGCC GGC^GCC SgrAI 1892 CACCGGTG C[GA]^CCGG[CT]G SmaI 2319 CCCGGG CCC^GGG SnaBI 3495 TACGTA TAC^GTA SpeI 5866 ACTAGT A^CTAGT Sse8387I 5896 CCTGCAGG CCTGCA^GG Tth111I 2190 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 4445 CCATGGCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2319 CCCGGG C^CCGGG XmaI 2319 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 206 GGCGCC G[GA]^CG[CT]C 2871 GACGTC Aor51HI 2424 AGCGCT AGC^GCT 5773 AGCGCT BamHI 2786 GGATCC G^GATCC 3817 GGATCC BglI 589 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4450 GCCTCTGTGGC BglII 2981 AGATCT A^GATCT 5973 AGATCT BsaBI 1529 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5968 GATACAGATC BsaHI 206 GGCGCC G[GA]^CG[CT]C 2871 GACGTC BsrBI 23 GAGCGG CCGCTC(-3/-3) 593 GAGCGG BsrFI 1425 ACCGGC [GA]^CCGG[CT] 1893 ACCGGT Cfr10I 1425 ACCGGC [GA]^CCGG[CT] 1893 ACCGGT Eco47III 2424 AGCGCT AGC^GCT 5773 AGCGCT EcoRI 40 GAATTC G^AATTC 840 GAATTC EcoRV 1082 GATATC GAT^ATC 3073 GATATC Hin1I 206 GGCGCC G[GA]^CG[CT]C 2871 GACGTC HindIII 2031 AAGCTT A^AGCTT 4536 AAGCTT MamI 1529 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5968 GATACAGATC MslI 5935 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 6101 CATCGAGATG PaeR7I 3985 CTCGAG C^TCGAG 5359 CTCGAG PshAI 146 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1829 GACACCTGTC PstI 1371 CTGCAG CTGCA^G 5897 CTGCAG SacI 1458 GAGCTC GAGCT^C 3578 GAGCTC SalI 1836 GTCGAC G^TCGAC 2868 GTCGAC SapI 679 GAAGAGC GCTCTTC(1/4) 4147 GAAGAGC SphI 130 GCATGC GCATG^C 2881 GCATGC StuI 1277 AGGCCT AGG^CCT 2225 AGGCCT XbaI 3533 TCTAGA T^CTAGA 4966 TCTAGA XhoI 3985 CTCGAG C^TCGAG 5359 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI