##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AccII AccIII AclI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BlnI
BlpI Bpu1102I BseAI BsiWI BspEI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII CpoI DrdI EagI
Eam1105I EclXI Eco52I FseI FspI
KpnI KspI MluI MroI MvnI
NaeI NgoMIV NheI NotI NruI
NspV PacI PflMI PmeI PshBI
Psp1406I RsrII SacII SexAI SfiI
SfuI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2871 GACGTC GACGT^C
AflII 3885 CTTAAG C^TTAAG
AgeI 1893 ACCGGT A^CCGGT
AocI 5257 CCTGAGG CC^T[ACGT]AGG
ApaI 4440 GGGCCC GGGCC^C
AspI 2190 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 206 GGCGCC GGCGC^C
BbrPI 3655 CACGTG CAC^GTG
BciVI 2611 GGATAC GTATCC(6/5)
BclI 1775 TGATCA T^GATCA
BfrI 3885 CTTAAG C^TTAAG
BsiEI 1598 CGATCG CG[GA][CT]^CG
BspDI 1596 ATCGAT AT^CGAT
BspHI 511 TCATGA T^CATGA
BspLU11I 5617 ACATGT A^CATGT
BstAPI 432 GCAAAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4083 GGTTACC G^GT[ACGT]ACC
BstPI 4083 GGTTACC G^GT[ACGT]ACC
BstXI 5934 CCACCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5257 CCTGAGG CC^T[ACGT]AGG
ClaI 1596 ATCGAT AT^CGAT
Eco81I 5257 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4083 GGTTACC G^GT[ACGT]ACC
EcoT22I 2858 ATGCAT ATGCA^T
FbaI 1775 TGATCA T^GATCA
HgaI 1258 GCGTC GACGC(5/10)
HpaI 615 GTTAAC GTT^AAC
KasI 206 GGCGCC G^GCGCC
MfeI 5478 CAATTG C^AATTG
MunI 5478 CAATTG C^AATTG
NarI 206 GGCGCC GG^CGCC
NsiI 2858 ATGCAT ATGCA^T
PflFI 2190 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1893 ACCGGT A^CCGGT
PmaCI 3655 CACGTG CAC^GTG
PmlI 3655 CACGTG CAC^GTG
PvuI 1598 CGATCG CGAT^CG
RcaI 511 TCATGA T^CATGA
SbfI 5896 CCTGCAGG CCTGCA^GG
ScaI 1793 AGTACT AGT^ACT
SfoI 206 GGCGCC GGC^GCC
SgrAI 1892 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2319 CCCGGG CCC^GGG
SnaBI 3495 TACGTA TAC^GTA
SpeI 5866 ACTAGT A^CTAGT
Sse8387I 5896 CCTGCAGG CCTGCA^GG
Tth111I 2190 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4445 CCATGGCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2319 CCCGGG C^CCGGG
XmaI 2319 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 206 GGCGCC G[GA]^CG[CT]C
2871 GACGTC
Aor51HI 2424 AGCGCT AGC^GCT
5773 AGCGCT
BamHI 2786 GGATCC G^GATCC
3817 GGATCC
BglI 589 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4450 GCCTCTGTGGC
BglII 2981 AGATCT A^GATCT
5973 AGATCT
BsaBI 1529 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5968 GATACAGATC
BsaHI 206 GGCGCC G[GA]^CG[CT]C
2871 GACGTC
BsrBI 23 GAGCGG CCGCTC(-3/-3)
593 GAGCGG
BsrFI 1425 ACCGGC [GA]^CCGG[CT]
1893 ACCGGT
Cfr10I 1425 ACCGGC [GA]^CCGG[CT]
1893 ACCGGT
Eco47III 2424 AGCGCT AGC^GCT
5773 AGCGCT
EcoRI 40 GAATTC G^AATTC
840 GAATTC
EcoRV 1082 GATATC GAT^ATC
3073 GATATC
Hin1I 206 GGCGCC G[GA]^CG[CT]C
2871 GACGTC
HindIII 2031 AAGCTT A^AGCTT
4536 AAGCTT
MamI 1529 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5968 GATACAGATC
MslI 5935 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
6101 CATCGAGATG
PaeR7I 3985 CTCGAG C^TCGAG
5359 CTCGAG
PshAI 146 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1829 GACACCTGTC
PstI 1371 CTGCAG CTGCA^G
5897 CTGCAG
SacI 1458 GAGCTC GAGCT^C
3578 GAGCTC
SalI 1836 GTCGAC G^TCGAC
2868 GTCGAC
SapI 679 GAAGAGC GCTCTTC(1/4)
4147 GAAGAGC
SphI 130 GCATGC GCATG^C
2881 GCATGC
StuI 1277 AGGCCT AGG^CCT
2225 AGGCCT
XbaI 3533 TCTAGA T^CTAGA
4966 TCTAGA
XhoI 3985 CTCGAG C^TCGAG
5359 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI