##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AgeI Aor51HI AscI
AseI AsnI Asp718I AviII BamHI
BglI BglII BsaBI BsiEI BsiWI
BspDI BsrFI BssHII BstEII BstPI
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoT22I FseI
FspI HincII HindII HpaI KpnI
KspI MamI NaeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 1426 AACGTT AA^CGTT
AcyI 3465 GGCGCC G[GA]^CG[CT]C
AflII 214 CTTAAG C^TTAAG
AhdI 2665 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2665 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5050 CCTAGG C^CTAGG
BbeI 3465 GGCGCC GGCGC^C
BclI 2732 TGATCA T^GATCA
BfrI 214 CTTAAG C^TTAAG
BlnI 5050 CCTAGG C^CTAGG
BsaHI 3465 GGCGCC G[GA]^CG[CT]C
BsmBI 4322 GAGACG CGTCTC(1/5)
BsmI 2100 GAATGC GAATGC(1/-1)
Bsp1407I 3202 TGTACA T^GTACA
BsrBI 3498 GAGCGG CCGCTC(-3/-3)
BsrGI 3202 TGTACA T^GTACA
BssSI 4926 CACGAG CACGAG(-5/-1)
BstBI 3625 TTCGAA TT^CGAA
DrdI 1624 GACCCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2665 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3244 GATATC GAT^ATC
FbaI 2732 TGATCA T^GATCA
Hin1I 3465 GGCGCC G[GA]^CG[CT]C
HindIII 4466 AAGCTT A^AGCTT
KasI 3465 GGCGCC G^GCGCC
MfeI 3481 CAATTG C^AATTG
MluI 803 ACGCGT A^CGCGT
MunI 3481 CAATTG C^AATTG
NarI 3465 GGCGCC GG^CGCC
NdeI 5197 CATATG CA^TATG
NspV 3625 TTCGAA TT^CGAA
PflMI 5423 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4671 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1426 AACGTT AA^CGTT
PvuII 2558 CAGCTG CAG^CTG
SfoI 3465 GGCGCC GGC^GCC
SfuI 3625 TTCGAA TT^CGAA
SphI 2162 GCATGC GCATG^C
SspBI 3202 TGTACA T^GTACA
SspI 2473 AATATT AAT^ATT
StuI 4245 AGGCCT AGG^CCT
Van91I 5423 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4107 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 540 TCCGGA T^CCGGA
4403 TCCGGA
Alw44I 2362 GTGCAC G^TGCAC
3015 GTGCAC
ApaI 5404 GGGCCC GGGCC^C
5506 GGGCCC
ApaLI 2362 GTGCAC G^TGCAC
3015 GTGCAC
AspI 1522 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4341 GACTCTGTC
BbrPI 1915 CACGTG CAC^GTG
2820 CACGTG
BbvCI 3117 GCTGAGG CCTCAGC(-5/-2)
3965 GCTGAGG
BcgI 776 GCAGCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2104 GCAGTGGTGTCG
BciVI 822 GTATCC GTATCC(6/5)
4767 GTATCC
BlpI 3077 GCTGAGC GC^T[ACGT]AGC
5516 GCTTAGC
Bpu1102I 3077 GCTGAGC GC^T[ACGT]AGC
5516 GCTTAGC
BsaAI 1915 CACGTG [CT]AC^GT[GA]
2820 CACGTG
BsaWI 540 TCCGGA [AT]^CCGG[AT]
4403 TCCGGA
BseAI 540 TCCGGA T^CCGGA
4403 TCCGGA
BseRI 2136 CTCCTC GAGGAG(10/8)
2177 CTCCTC
BspEI 540 TCCGGA T^CCGGA
4403 TCCGGA
BsrDI 3860 CATTGC GCAATG(2/0)
3124 GCAATG
Bst1107I 915 GTATAC GTA^TAC
2884 GTATAC
BstZ17I 915 GTATAC GTA^TAC
2884 GTATAC
CelII 3077 GCTGAGC GC^T[ACGT]AGC
5516 GCTTAGC
DraIII 3025 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5112 CACATTGTG
EcoNI 3900 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4629 CCTGAATCAGG
HaeII 3048 GGCGCT [GA]GCGC^[CT]
3465 GGCGCC
HgaI 2341 GACGC GACGC(5/10)
779 GCGTC
MroI 540 TCCGGA T^CCGGA
4403 TCCGGA
PflFI 1522 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4341 GACTCTGTC
PmaCI 1915 CACGTG CAC^GTG
2820 CACGTG
PmlI 1915 CACGTG CAC^GTG
2820 CACGTG
SapI 2912 GAAGAGC GCTCTTC(1/4)
4643 GCTCTTC
ScaI 1208 AGTACT AGT^ACT
1236 AGTACT
SmaI 1267 CCCGGG CCC^GGG
1991 CCCGGG
Tth111I 1522 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4341 GACTCTGTC
XcmI 1607 CCAAAGGTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4464 CCAAGCTTCTTTTGG
XmaCI 1267 CCCGGG C^CCGGG
1991 CCCGGG
XmaI 1267 CCCGGG C^CCGGG
1991 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI