################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AgeI Aor51HI AscI AseI AsnI Asp718I AviII BamHI BglI BglII BsaBI BsiEI BsiWI BspDI BsrFI BssHII BstEII BstPI Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HincII HindII HpaI KpnI KspI MamI NaeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 1426 AACGTT AA^CGTT AcyI 3465 GGCGCC G[GA]^CG[CT]C AflII 214 CTTAAG C^TTAAG AhdI 2665 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2665 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 5050 CCTAGG C^CTAGG BbeI 3465 GGCGCC GGCGC^C BclI 2732 TGATCA T^GATCA BfrI 214 CTTAAG C^TTAAG BlnI 5050 CCTAGG C^CTAGG BsaHI 3465 GGCGCC G[GA]^CG[CT]C BsmBI 4322 GAGACG CGTCTC(1/5) BsmI 2100 GAATGC GAATGC(1/-1) Bsp1407I 3202 TGTACA T^GTACA BsrBI 3498 GAGCGG CCGCTC(-3/-3) BsrGI 3202 TGTACA T^GTACA BssSI 4926 CACGAG CACGAG(-5/-1) BstBI 3625 TTCGAA TT^CGAA DrdI 1624 GACCCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2665 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 3244 GATATC GAT^ATC FbaI 2732 TGATCA T^GATCA Hin1I 3465 GGCGCC G[GA]^CG[CT]C HindIII 4466 AAGCTT A^AGCTT KasI 3465 GGCGCC G^GCGCC MfeI 3481 CAATTG C^AATTG MluI 803 ACGCGT A^CGCGT MunI 3481 CAATTG C^AATTG NarI 3465 GGCGCC GG^CGCC NdeI 5197 CATATG CA^TATG NspV 3625 TTCGAA TT^CGAA PflMI 5423 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4671 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1426 AACGTT AA^CGTT PvuII 2558 CAGCTG CAG^CTG SfoI 3465 GGCGCC GGC^GCC SfuI 3625 TTCGAA TT^CGAA SphI 2162 GCATGC GCATG^C SspBI 3202 TGTACA T^GTACA SspI 2473 AATATT AAT^ATT StuI 4245 AGGCCT AGG^CCT Van91I 5423 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4107 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccIII 540 TCCGGA T^CCGGA 4403 TCCGGA Alw44I 2362 GTGCAC G^TGCAC 3015 GTGCAC ApaI 5404 GGGCCC GGGCC^C 5506 GGGCCC ApaLI 2362 GTGCAC G^TGCAC 3015 GTGCAC AspI 1522 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4341 GACTCTGTC BbrPI 1915 CACGTG CAC^GTG 2820 CACGTG BbvCI 3117 GCTGAGG CCTCAGC(-5/-2) 3965 GCTGAGG BcgI 776 GCAGCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2104 GCAGTGGTGTCG BciVI 822 GTATCC GTATCC(6/5) 4767 GTATCC BlpI 3077 GCTGAGC GC^T[ACGT]AGC 5516 GCTTAGC Bpu1102I 3077 GCTGAGC GC^T[ACGT]AGC 5516 GCTTAGC BsaAI 1915 CACGTG [CT]AC^GT[GA] 2820 CACGTG BsaWI 540 TCCGGA [AT]^CCGG[AT] 4403 TCCGGA BseAI 540 TCCGGA T^CCGGA 4403 TCCGGA BseRI 2136 CTCCTC GAGGAG(10/8) 2177 CTCCTC BspEI 540 TCCGGA T^CCGGA 4403 TCCGGA BsrDI 3860 CATTGC GCAATG(2/0) 3124 GCAATG Bst1107I 915 GTATAC GTA^TAC 2884 GTATAC BstZ17I 915 GTATAC GTA^TAC 2884 GTATAC CelII 3077 GCTGAGC GC^T[ACGT]AGC 5516 GCTTAGC DraIII 3025 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 5112 CACATTGTG EcoNI 3900 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4629 CCTGAATCAGG HaeII 3048 GGCGCT [GA]GCGC^[CT] 3465 GGCGCC HgaI 2341 GACGC GACGC(5/10) 779 GCGTC MroI 540 TCCGGA T^CCGGA 4403 TCCGGA PflFI 1522 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4341 GACTCTGTC PmaCI 1915 CACGTG CAC^GTG 2820 CACGTG PmlI 1915 CACGTG CAC^GTG 2820 CACGTG SapI 2912 GAAGAGC GCTCTTC(1/4) 4643 GCTCTTC ScaI 1208 AGTACT AGT^ACT 1236 AGTACT SmaI 1267 CCCGGG CCC^GGG 1991 CCCGGG Tth111I 1522 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4341 GACTCTGTC XcmI 1607 CCAAAGGTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4464 CCAAGCTTCTTTTGG XmaCI 1267 CCCGGG C^CCGGG 1991 CCCGGG XmaI 1267 CCCGGG C^CCGGG 1991 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI