##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AscI AseI AsnI BbrPI BsaAI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BssHII Bst1107I BstBI BstUI
BstZ17I ClaI CpoI EcoRI HincII
HindII HpaI KspI MamI MfeI
MluI MroI MunI MvnI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfuI SnaBI SphI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 368 GGTACC G^GTACC
AcyI 229 GGCGCC G[GA]^CG[CT]C
AflII 1963 CTTAAG C^TTAAG
AhdI 3169 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1171 GTGCAC G^TGCAC
Aor51HI 2735 AGCGCT AGC^GCT
ApaI 1738 GGGCCC GGGCC^C
ApaLI 1171 GTGCAC G^TGCAC
Asp700I 3241 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 368 GGTACC G^GTACC
AspEI 3169 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 179 TGCGCA TGC^GCA
AvrII 1716 CCTAGG C^CTAGG
BamHI 2771 GGATCC G^GATCC
BbeI 229 GGCGCC GGCGC^C
BcgI 3962 CGAGTGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2704 GTATCC GTATCC(6/5)
BclI 3073 TGATCA T^GATCA
BfrI 1963 CTTAAG C^TTAAG
BglII 4376 AGATCT A^GATCT
BlnI 1716 CCTAGG C^CTAGG
BlpI 2581 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2581 GCTGAGC GC^T[ACGT]AGC
BsaHI 229 GGCGCC G[GA]^CG[CT]C
BsaWI 3495 ACCGGA [AT]^CCGG[AT]
BsiEI 1053 CGGCCG CG[GA][CT]^CG
BsmBI 3917 GAGACG CGTCTC(1/5)
BspHI 4583 TCATGA T^CATGA
BsrBI 2791 GAGCGG CCGCTC(-3/-3)
BssSI 2078 CTCGTG CACGAG(-5/-1)
BstEII 1451 GGTCACC G^GT[ACGT]ACC
BstPI 1451 GGTCACC G^GT[ACGT]ACC
CelII 2581 GCTGAGC GC^T[ACGT]AGC
DraI 3710 TTTAAA TTT^AAA
EagI 1053 CGGCCG C^GGCCG
Eam1105I 3169 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1053 CGGCCG C^GGCCG
Eco47III 2735 AGCGCT AGC^GCT
Eco52I 1053 CGGCCG C^GGCCG
EcoO65I 1451 GGTCACC G^GT[ACGT]ACC
EcoRV 2879 GATATC GAT^ATC
FbaI 3073 TGATCA T^GATCA
FseI 1105 GGCCGGCC GGCCGG^CC
FspI 179 TGCGCA TGC^GCA
Hin1I 229 GGCGCC G[GA]^CG[CT]C
HindIII 3373 AAGCTT A^AGCTT
KasI 229 GGCGCC G^GCGCC
KpnI 368 GGTACC GGTAC^C
NarI 229 GGCGCC GG^CGCC
NdeI 4069 CATATG CA^TATG
RcaI 4583 TCATGA T^CATGA
SbfI 3030 CCTGCAGG CCTGCA^GG
SfoI 229 GGCGCC GGC^GCC
SgrAI 2472 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 1963 CTTAAG C^T[CT][GA]AG
SpeI 573 ACTAGT A^CTAGT
Sse8387I 3030 CCTGCAGG CCTGCA^GG
SspI 4331 AATATT AAT^ATT
XmnI 3241 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspI 196 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
925 GACTTTGTC
BspLU11I 4078 ACATGT A^CATGT
4430 ACATGT
BsrDI 2949 CATTGC GCAATG(2/0)
3400 CATTGC
DrdI 1224 GACAGCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4202 GACCTGCCAGTC
EcoT22I 3508 ATGCAT ATGCA^T
4233 ATGCAT
HgaI 3589 GACGC GACGC(5/10)
1554 GCGTC
MaeII 3707 ACGT A^CGT
4308 ACGT
NaeI 1106 GCCGGC GCC^GGC
1652 GCCGGC
NgoMIV 1106 GCCGGC G^CCGGC
1652 GCCGGC
NsiI 3508 ATGCAT ATGCA^T
4233 ATGCAT
PflFI 196 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
925 GACTTTGTC
SacI 1017 GAGCTC GAGCT^C
2075 GAGCTC
SapI 3308 GAAGAGC GCTCTTC(1/4)
2186 GCTCTTC
SexAI 125 ACCTGGT A^CC[AT]GGT
4509 ACCTGGT
SfiI 1105 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1317 GGCCATGCTGGCC
Tth111I 196 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
925 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI