##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccII AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbeI BsiEI BsmBI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I CfoI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI FspI HaeII HhaI
HinP1I KasI KpnI KspI MfeI
MluI MunI MvnI NaeI NarI
NgoMIV NotI NruI NspV PacI
PflFI PinAI PmeI PshBI PvuI
RcaI RsrII SacII SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3400 GACGTC GACGT^C
AccIII 3626 TCCGGA T^CCGGA
AclI 5153 AACGTT AA^CGTT
AcyI 3400 GACGTC G[GA]^CG[CT]C
AflII 5361 CTTAAG C^TTAAG
AhdI 922 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 922 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4208 CCTAGG C^CTAGG
BbrPI 2380 CACGTG CAC^GTG
BcgI 3553 CGAGAGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5361 CTTAAG C^TTAAG
BglI 970 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3423 AGATCT A^GATCT
BlnI 4208 CCTAGG C^CTAGG
BsaAI 2380 CACGTG [CT]AC^GT[GA]
BsaBI 876 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3400 GACGTC G[GA]^CG[CT]C
BsaWI 3626 TCCGGA [AT]^CCGG[AT]
BseAI 3626 TCCGGA T^CCGGA
BsiWI 1013 CGTACG C^GTACG
BspEI 3626 TCCGGA T^CCGGA
BspLU11I 1989 ACATGT A^CATGT
BsrFI 2474 ACCGGC [GA]^CCGG[CT]
BssSI 4127 CACGAG CACGAG(-5/-1)
BstEII 4014 GGTAACC G^GT[ACGT]ACC
BstPI 4014 GGTAACC G^GT[ACGT]ACC
BstXI 5029 CCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2474 ACCGGC [GA]^CCGG[CT]
Eam1105I 922 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4014 GGTAACC G^GT[ACGT]ACC
EcoRI 4997 GAATTC G^AATTC
FauI 4086 GCGGG CCCGC(4/6)
Hin1I 3400 GACGTC G[GA]^CG[CT]C
HpaI 5156 GTTAAC GTT^AAC
MamI 876 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3626 TCCGGA T^CCGGA
NheI 2877 GCTAGC G^CTAGC
PaeR7I 3947 CTCGAG C^TCGAG
PmaCI 2380 CACGTG CAC^GTG
PmlI 2380 CACGTG CAC^GTG
PpuMI 443 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 5153 AACGTT AA^CGTT
SalI 1818 GTCGAC G^TCGAC
SapI 3756 GCTCTTC GCTCTTC(1/4)
SbfI 654 CCTGCAGG CCTGCA^GG
ScaI 2973 AGTACT AGT^ACT
SexAI 1581 ACCAGGT A^CC[AT]GGT
Sse8387I 654 CCTGCAGG CCTGCA^GG
SspI 4247 AATATT AAT^ATT
XhoI 3947 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 4676 GTGCAC G^TGCAC
5042 GTGCAC
AocI 1109 CCTCAGG CC^T[ACGT]AGG
3024 CCTCAGG
ApaI 1378 GGGCCC GGGCC^C
5025 GGGCCC
ApaLI 4676 GTGCAC G^TGCAC
5042 GTGCAC
Asp700I 1720 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3816 GAACTCCTTC
AvaI 3716 CTCGGG C^[CT]CG[GA]G
3947 CTCGAG
BanI 1032 GGTGCC G^G[CT][GA]CC
2914 GGCACC
BbvCI 1081 GCTGAGG CCTCAGC(-5/-2)
3952 GCTGAGG
BclI 827 TGATCA T^GATCA
5303 TGATCA
BcnI 2942 CCGGG CC^[GC]GG
3564 CCGGG
BsoBI 3716 CTCGGG C^[CT]CG[GA]G
3947 CTCGAG
BsrBI 2845 GAGCGG CCGCTC(-3/-3)
4084 GAGCGG
Bsu36I 1109 CCTCAGG CC^T[ACGT]AGG
3024 CCTCAGG
DraI 1245 TTTAAA TTT^AAA
4501 TTTAAA
Eco81I 1109 CCTCAGG CC^T[ACGT]AGG
3024 CCTCAGG
FbaI 827 TGATCA T^GATCA
5303 TGATCA
HindIII 2744 AAGCTT A^AGCTT
2926 AAGCTT
NciI 2942 CCGGG CC^[GC]GG
3564 CCGGG
NdeI 2386 CATATG CA^TATG
5267 CATATG
PflMI 522 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2454 CCAGTTCTTGG
SphI 780 GCATGC GCATG^C
4606 GCATGC
Van91I 522 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2454 CCAGTTCTTGG
XcmI 1541 CCAGAGCTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2900 CCATGATGCAGATGG
XmnI 1720 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3816 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BciVI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshAI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII