################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AclI AflII AgeI ApaI AscI AseI AsnI AvrII BfrI BlnI BsiEI BsiWI BsmBI BspDI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco52I FseI HpaI KspI MfeI MluI MunI NaeI NgoMIV NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SfuI SgrAI SmaI SnaBI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 45 GGTACC G^GTACC AccIII 3968 TCCGGA T^CCGGA AhdI 968 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4445 AGCGCT AGC^GCT Asp718I 45 GGTACC G^GTACC AspEI 968 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2693 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2041 TGCGCA TGC^GCA BamHI 3376 GGATCC G^GATCC BbeI 1699 GGCGCC GGCGC^C BcgI 5208 GCACAGTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BseAI 3968 TCCGGA T^CCGGA Bsp1407I 1014 TGTACA T^GTACA BspEI 3968 TCCGGA T^CCGGA BsrDI 1156 GCAATG GCAATG(2/0) BsrGI 1014 TGTACA T^GTACA DraI 4851 TTTAAA TTT^AAA Eam1105I 968 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4445 AGCGCT AGC^GCT EcoT22I 2110 ATGCAT ATGCA^T FspI 2041 TGCGCA TGC^GCA HindIII 1918 AAGCTT A^AGCTT KasI 1699 GGCGCC G^GCGCC KpnI 45 GGTACC GGTAC^C MroI 3968 TCCGGA T^CCGGA NarI 1699 GGCGCC GG^CGCC NheI 2226 GCTAGC G^CTAGC NsiI 2110 ATGCAT ATGCA^T PaeR7I 590 CTCGAG C^TCGAG PflFI 2693 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4534 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 4560 CCTGCAGG CCTGCA^GG ScaI 66 AGTACT AGT^ACT SexAI 3974 ACCTGGT A^CC[AT]GGT SfiI 3319 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1699 GGCGCC GGC^GCC Sse8387I 4560 CCTGCAGG CCTGCA^GG SspBI 1014 TGTACA T^GTACA Tth111I 2693 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 590 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 1498 GACGTC GACGT^C 4161 GACGTC AccII 293 CGCG CG^CG 4059 CGCG AflIII 4973 ACGTGT A^C[GA][CT]GT 5286 ACACGT Asp700I 3915 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4509 GAATTCTTTC AvaI 590 CTCGAG C^[CT]CG[GA]G 4549 CCCGAG BalI 576 TGGCCA TGG^CCA 3318 TGGCCA BbrPI 719 CACGTG CAC^GTG 4972 CACGTG BbsI 731 GAAGAC GAAGAC(2/6) 266 GTCTTC BciVI 2623 GGATAC GTATCC(6/5) 4403 GTATCC BclI 1532 TGATCA T^GATCA 4589 TGATCA BpuAI 731 GAAGAC GAAGAC(2/6) 266 GTCTTC BsaAI 719 CACGTG [CT]AC^GT[GA] 4972 CACGTG BsaBI 2165 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5025 GATCCACATC BsaWI 3968 TCCGGA [AT]^CCGG[AT] 4439 ACCGGA BsgI 1270 GTGCAG GTGCAG(16/14) 1401 GTGCAG BsoBI 590 CTCGAG C^[CT]CG[GA]G 4549 CCCGAG BssSI 4786 CACGAG CACGAG(-5/-1) 56 CTCGTG BstUI 293 CGCG CG^CG 4059 CGCG DrdI 257 GACATGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1826 GACAACTGGGTC EcoRI 4473 GAATTC G^AATTC 4509 GAATTC FauI 5180 GCGGG CCCGC(4/6) 5201 GCGGG FbaI 1532 TGATCA T^GATCA 4589 TGATCA MamI 2165 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5025 GATCCACATC MluNI 576 TGGCCA TGG^CCA 3318 TGGCCA MscI 576 TGGCCA TGG^CCA 3318 TGGCCA MvnI 293 CGCG CG^CG 4059 CGCG NdeI 2338 CATATG CA^TATG 3435 CATATG PmaCI 719 CACGTG CAC^GTG 4972 CACGTG PmlI 719 CACGTG CAC^GTG 4972 CACGTG SacI 587 GAGCTC GAGCT^C 4101 GAGCTC SpeI 2938 ACTAGT A^CTAGT 3142 ACTAGT XmnI 3915 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4509 GAATTCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII