##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AclI AflII AgeI ApaI AscI
AseI AsnI AvrII BfrI BlnI
BsiEI BsiWI BsmBI BspDI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI EagI
EclXI Eco52I FseI HpaI KspI
MfeI MluI MunI NaeI NgoMIV
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfuI SgrAI SmaI
SnaBI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 45 GGTACC G^GTACC
AccIII 3968 TCCGGA T^CCGGA
AhdI 968 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4445 AGCGCT AGC^GCT
Asp718I 45 GGTACC G^GTACC
AspEI 968 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2693 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2041 TGCGCA TGC^GCA
BamHI 3376 GGATCC G^GATCC
BbeI 1699 GGCGCC GGCGC^C
BcgI 5208 GCACAGTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 3968 TCCGGA T^CCGGA
Bsp1407I 1014 TGTACA T^GTACA
BspEI 3968 TCCGGA T^CCGGA
BsrDI 1156 GCAATG GCAATG(2/0)
BsrGI 1014 TGTACA T^GTACA
DraI 4851 TTTAAA TTT^AAA
Eam1105I 968 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4445 AGCGCT AGC^GCT
EcoT22I 2110 ATGCAT ATGCA^T
FspI 2041 TGCGCA TGC^GCA
HindIII 1918 AAGCTT A^AGCTT
KasI 1699 GGCGCC G^GCGCC
KpnI 45 GGTACC GGTAC^C
MroI 3968 TCCGGA T^CCGGA
NarI 1699 GGCGCC GG^CGCC
NheI 2226 GCTAGC G^CTAGC
NsiI 2110 ATGCAT ATGCA^T
PaeR7I 590 CTCGAG C^TCGAG
PflFI 2693 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4534 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4560 CCTGCAGG CCTGCA^GG
ScaI 66 AGTACT AGT^ACT
SexAI 3974 ACCTGGT A^CC[AT]GGT
SfiI 3319 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1699 GGCGCC GGC^GCC
Sse8387I 4560 CCTGCAGG CCTGCA^GG
SspBI 1014 TGTACA T^GTACA
Tth111I 2693 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 590 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1498 GACGTC GACGT^C
4161 GACGTC
AccII 293 CGCG CG^CG
4059 CGCG
AflIII 4973 ACGTGT A^C[GA][CT]GT
5286 ACACGT
Asp700I 3915 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4509 GAATTCTTTC
AvaI 590 CTCGAG C^[CT]CG[GA]G
4549 CCCGAG
BalI 576 TGGCCA TGG^CCA
3318 TGGCCA
BbrPI 719 CACGTG CAC^GTG
4972 CACGTG
BbsI 731 GAAGAC GAAGAC(2/6)
266 GTCTTC
BciVI 2623 GGATAC GTATCC(6/5)
4403 GTATCC
BclI 1532 TGATCA T^GATCA
4589 TGATCA
BpuAI 731 GAAGAC GAAGAC(2/6)
266 GTCTTC
BsaAI 719 CACGTG [CT]AC^GT[GA]
4972 CACGTG
BsaBI 2165 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5025 GATCCACATC
BsaWI 3968 TCCGGA [AT]^CCGG[AT]
4439 ACCGGA
BsgI 1270 GTGCAG GTGCAG(16/14)
1401 GTGCAG
BsoBI 590 CTCGAG C^[CT]CG[GA]G
4549 CCCGAG
BssSI 4786 CACGAG CACGAG(-5/-1)
56 CTCGTG
BstUI 293 CGCG CG^CG
4059 CGCG
DrdI 257 GACATGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1826 GACAACTGGGTC
EcoRI 4473 GAATTC G^AATTC
4509 GAATTC
FauI 5180 GCGGG CCCGC(4/6)
5201 GCGGG
FbaI 1532 TGATCA T^GATCA
4589 TGATCA
MamI 2165 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5025 GATCCACATC
MluNI 576 TGGCCA TGG^CCA
3318 TGGCCA
MscI 576 TGGCCA TGG^CCA
3318 TGGCCA
MvnI 293 CGCG CG^CG
4059 CGCG
NdeI 2338 CATATG CA^TATG
3435 CATATG
PmaCI 719 CACGTG CAC^GTG
4972 CACGTG
PmlI 719 CACGTG CAC^GTG
4972 CACGTG
SacI 587 GAGCTC GAGCT^C
4101 GAGCTC
SpeI 2938 ACTAGT A^CTAGT
3142 ACTAGT
XmnI 3915 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4509 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII