##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccIII AclI AcsI Alw44I
ApaLI ApoI AscI AseI AsnI
BbeI BseAI BsiWI BspDI BspEI
BssHII BssSI BstBI ClaI CpoI
DraIII EcoRI EcoRV EcoT22I FseI
HpaI KasI MfeI MluI MroI
MunI NarI NdeI NotI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RsrII SalI SfoI SfuI
SgrAI SmaI SpeI SphI SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3398 GGTACC G^GTACC
AccI 217 GTATAC GT^[AC][GT]AC
AflIII 1809 ACATGT A^C[GA][CT]GT
AgeI 2736 ACCGGT A^CCGGT
AhdI 1033 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 596 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3398 GGTACC G^GTACC
AspEI 1033 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 40 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1517 CACGTG CAC^GTG
BcgI 2194 GCATTCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5205 GTATCC GTATCC(6/5)
BsaBI 5130 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 185 CGGCCG CG[GA][CT]^CG
BspHI 826 TCATGA T^CATGA
BspLU11I 1809 ACATGT A^CATGT
Bst1107I 217 GTATAC GTA^TAC
BstZ17I 217 GTATAC GTA^TAC
DrdI 422 GACCACCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 185 CGGCCG C^GGCCG
Eam1105I 1033 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 185 CGGCCG C^GGCCG
Eco52I 185 CGGCCG C^GGCCG
HindIII 3262 AAGCTT A^AGCTT
KpnI 3398 GGTACC GGTAC^C
MamI 5130 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4523 GCCGGC GCC^GGC
NgoMIV 4523 GCCGGC G^CCGGC
NheI 3161 GCTAGC G^CTAGC
NruI 116 TCGCGA TCG^CGA
PaeR7I 1725 CTCGAG C^TCGAG
PflFI 40 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2736 ACCGGT A^CCGGT
PmaCI 1517 CACGTG CAC^GTG
PmlI 1517 CACGTG CAC^GTG
PshAI 3923 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 826 TCATGA T^CATGA
SfiI 186 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 588 TACGTA TAC^GTA
StuI 2440 AGGCCT AGG^CCT
Tth111I 40 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1725 CTCGAG C^TCGAG
XmnI 596 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 43 GGCGTC G[GA]^CG[CT]C
152 GACGCC
AflII 3026 CTTAAG C^TTAAG
3108 CTTAAG
Aor51HI 712 AGCGCT AGC^GCT
5448 AGCGCT
AviII 580 TGCGCA TGC^GCA
2969 TGCGCA
AvrII 1530 CCTAGG C^CTAGG
5026 CCTAGG
BclI 1102 TGATCA T^GATCA
4438 TGATCA
BfrI 3026 CTTAAG C^TTAAG
3108 CTTAAG
BlnI 1530 CCTAGG C^CTAGG
5026 CCTAGG
BsaAI 588 TACGTA [CT]AC^GT[GA]
1517 CACGTG
BsaHI 43 GGCGTC G[GA]^CG[CT]C
152 GACGCC
BsaWI 2736 ACCGGT [AT]^CCGG[AT]
4501 TCCGGT
BsrBI 1584 CCGCTC CCGCTC(-3/-3)
966 GAGCGG
BsrDI 959 CATTGC GCAATG(2/0)
3292 CATTGC
BsrFI 2736 ACCGGT [GA]^CCGG[CT]
4523 GCCGGC
BstEII 2667 GGTGACC G^GT[ACGT]ACC
3224 GGTAACC
BstPI 2667 GGTGACC G^GT[ACGT]ACC
3224 GGTAACC
Cfr10I 2736 ACCGGT [GA]^CCGG[CT]
4523 GCCGGC
DraI 5264 TTTAAA TTT^AAA
5540 TTTAAA
Eco47III 712 AGCGCT AGC^GCT
5448 AGCGCT
EcoO65I 2667 GGTGACC G^GT[ACGT]ACC
3224 GGTAACC
FauI 52 CCCGC CCCGC(4/6)
2463 CCCGC
FbaI 1102 TGATCA T^GATCA
4438 TGATCA
FspI 580 TGCGCA TGC^GCA
2969 TGCGCA
Hin1I 43 GGCGTC G[GA]^CG[CT]C
152 GACGCC
HincII 368 GTCAAC GT[CT]^[GA]AC
2078 GTCAAC
HindII 368 GTCAAC GT[CT]^[GA]AC
2078 GTCAAC
KspI 21 CCGCGG CCGC^GG
81 CCGCGG
SacII 21 CCGCGG CCGC^GG
81 CCGCGG
SbfI 2443 CCTGCAGG CCTGCA^GG
2584 CCTGCAGG
ScaI 652 AGTACT AGT^ACT
5495 AGTACT
SexAI 4095 ACCTGGT A^CC[AT]GGT
5257 ACCTGGT
Sse8387I 2443 CCTGCAGG CCTGCA^GG
2584 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII