##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AflII AgeI Aor51HI AscI
AseI AsnI Asp700I BbrPI BclI
BfrI BsaAI BseAI BsiWI BsmBI
BspDI BspEI Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRI FbaI
HpaI KspI MfeI MluI MroI
MunI NdeI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 3706 GACGTC GACGT^C
AclI 946 AACGTT AA^CGTT
AcsI 4889 GAATTT [GA]^AATT[CT]
Alw44I 1110 GTGCAC G^TGCAC
ApaLI 1110 GTGCAC G^TGCAC
ApoI 4889 GAATTT [GA]^AATT[CT]
AspI 5691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2885 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 5962 AGATCT A^GATCT
BlpI 1841 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1841 GCTGAGC GC^T[ACGT]AGC
BsaBI 3887 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2634 TCATGA T^CATGA
BsrBI 2452 GAGCGG CCGCTC(-3/-3)
BssSI 2883 CACGAG CACGAG(-5/-1)
CelII 1841 GCTGAGC GC^T[ACGT]AGC
EagI 3205 CGGCCG C^GGCCG
EclXI 3205 CGGCCG C^GGCCG
Eco52I 3205 CGGCCG C^GGCCG
EcoRV 1877 GATATC GAT^ATC
EcoT22I 2053 ATGCAT ATGCA^T
HincII 666 GTTGAC GT[CT]^[GA]AC
HindII 666 GTTGAC GT[CT]^[GA]AC
HindIII 2123 AAGCTT A^AGCTT
MamI 3887 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2053 ATGCAT ATGCA^T
PflFI 5691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 946 AACGTT AA^CGTT
RcaI 2634 TCATGA T^CATGA
SapI 3268 GAAGAGC GCTCTTC(1/4)
Tth111I 5691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 4181 GGTACC G^GTACC
4992 GGTACC
AccI 1547 GTAGAC GT^[AC][GT]AC
3815 GTCTAC
AflIII 1362 ACATGT A^C[GA][CT]GT
4802 ACATGT
Asp718I 4181 GGTACC G^GTACC
4992 GGTACC
AviII 532 TGCGCA TGC^GCA
4581 TGCGCA
BamHI 470 GGATCC G^GATCC
2931 GGATCC
BbeI 2148 GGCGCC GGCGC^C
3188 GGCGCC
BsaWI 414 ACCGGA [AT]^CCGG[AT]
2243 ACCGGA
BsiEI 3205 CGGCCG CG[GA][CT]^CG
3222 CGACCG
BsmI 2227 GAATGC GAATGC(1/-1)
3413 GCATTC
Bsp1407I 1359 TGTACA T^GTACA
3453 TGTACA
BspLU11I 1362 ACATGT A^CATGT
4802 ACATGT
BsrGI 1359 TGTACA T^GTACA
3453 TGTACA
EcoNI 3848 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3949 CCTGGGTAAGG
FseI 1297 GGCCGGCC GGCCGG^CC
2322 GGCCGGCC
FspI 532 TGCGCA TGC^GCA
4581 TGCGCA
KasI 2148 GGCGCC G^GCGCC
3188 GGCGCC
KpnI 4181 GGTACC GGTAC^C
4992 GGTACC
NarI 2148 GGCGCC GG^CGCC
3188 GGCGCC
PaeR7I 3859 CTCGAG C^TCGAG
5546 CTCGAG
SfoI 2148 GGCGCC GGC^GCC
3188 GGCGCC
SmaI 4494 CCCGGG CCC^GGG
5193 CCCGGG
SphI 681 GCATGC GCATG^C
3243 GCATGC
SspBI 1359 TGTACA T^GTACA
3453 TGTACA
TaqI 3860 TCGA T^CGA
5547 TCGA
TthHB8I 3860 TCGA T^CGA
5547 TCGA
XhoI 3859 CTCGAG C^TCGAG
5546 CTCGAG
XmaCI 4494 CCCGGG C^CCGGG
5193 CCCGGG
XmaI 4494 CCCGGG C^CCGGG
5193 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApaI
AspEI AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BlnI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssHII BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NheI NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII