################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AccIII AflII AgeI Aor51HI AscI AseI AsnI Asp700I BbrPI BclI BfrI BsaAI BseAI BsiWI BsmBI BspDI BspEI Bst1107I BstBI BstZ17I ClaI CpoI Eco47III EcoRI FbaI HpaI KspI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 3706 GACGTC GACGT^C AclI 946 AACGTT AA^CGTT AcsI 4889 GAATTT [GA]^AATT[CT] Alw44I 1110 GTGCAC G^TGCAC ApaLI 1110 GTGCAC G^TGCAC ApoI 4889 GAATTT [GA]^AATT[CT] AspI 5691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 2885 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 5962 AGATCT A^GATCT BlpI 1841 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1841 GCTGAGC GC^T[ACGT]AGC BsaBI 3887 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 2634 TCATGA T^CATGA BsrBI 2452 GAGCGG CCGCTC(-3/-3) BssSI 2883 CACGAG CACGAG(-5/-1) CelII 1841 GCTGAGC GC^T[ACGT]AGC EagI 3205 CGGCCG C^GGCCG EclXI 3205 CGGCCG C^GGCCG Eco52I 3205 CGGCCG C^GGCCG EcoRV 1877 GATATC GAT^ATC EcoT22I 2053 ATGCAT ATGCA^T HincII 666 GTTGAC GT[CT]^[GA]AC HindII 666 GTTGAC GT[CT]^[GA]AC HindIII 2123 AAGCTT A^AGCTT MamI 3887 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NsiI 2053 ATGCAT ATGCA^T PflFI 5691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 946 AACGTT AA^CGTT RcaI 2634 TCATGA T^CATGA SapI 3268 GAAGAGC GCTCTTC(1/4) Tth111I 5691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 4181 GGTACC G^GTACC 4992 GGTACC AccI 1547 GTAGAC GT^[AC][GT]AC 3815 GTCTAC AflIII 1362 ACATGT A^C[GA][CT]GT 4802 ACATGT Asp718I 4181 GGTACC G^GTACC 4992 GGTACC AviII 532 TGCGCA TGC^GCA 4581 TGCGCA BamHI 470 GGATCC G^GATCC 2931 GGATCC BbeI 2148 GGCGCC GGCGC^C 3188 GGCGCC BsaWI 414 ACCGGA [AT]^CCGG[AT] 2243 ACCGGA BsiEI 3205 CGGCCG CG[GA][CT]^CG 3222 CGACCG BsmI 2227 GAATGC GAATGC(1/-1) 3413 GCATTC Bsp1407I 1359 TGTACA T^GTACA 3453 TGTACA BspLU11I 1362 ACATGT A^CATGT 4802 ACATGT BsrGI 1359 TGTACA T^GTACA 3453 TGTACA EcoNI 3848 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3949 CCTGGGTAAGG FseI 1297 GGCCGGCC GGCCGG^CC 2322 GGCCGGCC FspI 532 TGCGCA TGC^GCA 4581 TGCGCA KasI 2148 GGCGCC G^GCGCC 3188 GGCGCC KpnI 4181 GGTACC GGTAC^C 4992 GGTACC NarI 2148 GGCGCC GG^CGCC 3188 GGCGCC PaeR7I 3859 CTCGAG C^TCGAG 5546 CTCGAG SfoI 2148 GGCGCC GGC^GCC 3188 GGCGCC SmaI 4494 CCCGGG CCC^GGG 5193 CCCGGG SphI 681 GCATGC GCATG^C 3243 GCATGC SspBI 1359 TGTACA T^GTACA 3453 TGTACA TaqI 3860 TCGA T^CGA 5547 TCGA TthHB8I 3860 TCGA T^CGA 5547 TCGA XhoI 3859 CTCGAG C^TCGAG 5546 CTCGAG XmaCI 4494 CCCGGG C^CCGGG 5193 CCCGGG XmaI 4494 CCCGGG C^CCGGG 5193 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI AspEI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssHII BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NheI NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII