##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AgeI AhdI ApaI
AscI AspEI AviII AvrII BbrPI
BclI BlnI BsaAI BsaBI BsaWI
BseAI BsmBI BspDI BspEI BssHII
BssSI BstAPI BstBI BstEII BstPI
ClaI CpoI DrdI Eam1105I EcoO65I
EcoRV FauI FbaI FseI FspI
HincII HindII HpaI KspI MamI
MluI MroI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 3048 GACGTC GACGT^C
Acc65I 2546 GGTACC G^GTACC
AccII 33 CGCG CG^CG
AlwNI 2201 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 100 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4101 AGCGCT AGC^GCT
AseI 3181 ATTAAT AT^TAAT
AsnI 3181 ATTAAT AT^TAAT
Asp718I 2546 GGTACC G^GTACC
BbeI 477 GGCGCC GGCGC^C
BcgI 68 GCAGAGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 638 GTATCC GTATCC(6/5)
BcnI 2732 CCGGG CC^[GC]GG
BglI 4973 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5061 AGATCT A^GATCT
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsiWI 3944 CGTACG C^GTACG
Bsp1407I 4253 TGTACA T^GTACA
BspLU11I 414 ACATGT A^CATGT
BsrBI 1039 CCGCTC CCGCTC(-3/-3)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BsrGI 4253 TGTACA T^GTACA
Bst1107I 4628 GTATAC GTA^TAC
BstUI 33 CGCG CG^CG
BstXI 1674 CCAGGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4628 GTATAC GTA^TAC
Bsu36I 100 CCTGAGG CC^T[ACGT]AGG
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco47III 4101 AGCGCT AGC^GCT
Eco52I 11 CGGCCG C^GGCCG
Eco81I 100 CCTGAGG CC^T[ACGT]AGG
KasI 477 GGCGCC G^GCGCC
KpnI 2546 GGTACC GGTAC^C
MfeI 4434 CAATTG C^AATTG
MunI 4434 CAATTG C^AATTG
MvnI 33 CGCG CG^CG
NaeI 13 GCCGGC GCC^GGC
NarI 477 GGCGCC GG^CGCC
NciI 2732 CCGGG CC^[GC]GG
NgoMIV 13 GCCGGC G^CCGGC
PflMI 3259 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3181 ATTAAT AT^TAAT
SacI 2520 GAGCTC GAGCT^C
ScaI 2140 AGTACT AGT^ACT
SfoI 477 GGCGCC GGC^GCC
SphI 1713 GCATGC GCATG^C
SspBI 4253 TGTACA T^GTACA
SspI 4245 AATATT AAT^ATT
Van91I 3259 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1818 CCAAGGTCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 890 CTTAAG C^TTAAG
5024 CTTAAG
AflIII 414 ACATGT A^C[GA][CT]GT
4343 ACGTGT
Alw44I 1765 GTGCAC G^TGCAC
4025 GTGCAC
ApaLI 1765 GTGCAC G^TGCAC
4025 GTGCAC
AspI 80 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1845 GACCCTGTC
BalI 1336 TGGCCA TGG^CCA
4860 TGGCCA
BamHI 19 GGATCC G^GATCC
169 GGATCC
BfrI 890 CTTAAG C^TTAAG
5024 CTTAAG
BlpI 2476 GCTGAGC GC^T[ACGT]AGC
2517 GCTGAGC
Bpu1102I 2476 GCTGAGC GC^T[ACGT]AGC
2517 GCTGAGC
BsaI 1843 GAGACC GGTCTC(1/5)
2381 GGTCTC
BspHI 781 TCATGA T^CATGA
2360 TCATGA
BsrDI 1447 CATTGC GCAATG(2/0)
1956 CATTGC
CelII 2476 GCTGAGC GC^T[ACGT]AGC
2517 GCTGAGC
DraII 1804 GGGACCT [GA]G^G[ACGT]CC[CT]
3793 GGGTCCC
EcoNI 2551 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4872 CCTGTATTAGG
EcoO109I 1804 GGGACCT [GA]G^G[ACGT]CC[CT]
3793 GGGTCCC
MluNI 1336 TGGCCA TGG^CCA
4860 TGGCCA
MscI 1336 TGGCCA TGG^CCA
4860 TGGCCA
NcoI 955 CCATGG C^CATGG
2857 CCATGG
NheI 1652 GCTAGC G^CTAGC
2129 GCTAGC
PflFI 80 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1845 GACCCTGTC
PpuMI 1804 GGGACCT [GA]G^G[AT]CC[CT]
3793 GGGTCCC
PstI 3704 CTGCAG CTGCA^G
4989 CTGCAG
RcaI 781 TCATGA T^CATGA
2360 TCATGA
SexAI 501 ACCTGGT A^CC[AT]GGT
648 ACCAGGT
Tth111I 80 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1845 GACCCTGTC
XbaI 1895 TCTAGA T^CTAGA
4657 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII DsaI EaeI EarI EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI