################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AviII AvrII BamHI BcgI BciVI BlnI BlpI Bpu1102I BsaWI BseAI BsiWI BsmBI BspEI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HpaI KpnI KspI MluI MroI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 715 GACGTC GACGT^C AocI 4164 CCTCAGG CC^T[ACGT]AGG AspI 1981 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 208 CACGTG CAC^GTG BbvCI 5332 CCTCAGC CCTCAGC(-5/-2) BsaBI 5076 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1735 GAGACC GGTCTC(1/5) BsiEI 49 CGACCG CG[GA][CT]^CG BsmI 804 GAATGC GAATGC(1/-1) BspDI 1276 ATCGAT AT^CGAT BspHI 3844 TCATGA T^CATGA BsrFI 51 ACCGGC [GA]^CCGG[CT] Bsu36I 4164 CCTCAGG CC^T[ACGT]AGG Cfr10I 51 ACCGGC [GA]^CCGG[CT] ClaI 1276 ATCGAT AT^CGAT Eco81I 4164 CCTCAGG CC^T[ACGT]AGG EcoNI 2137 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 5319 GAATTC G^AATTC EcoRV 3319 GATATC GAT^ATC EcoT22I 1364 ATGCAT ATGCA^T HgaI 307 GACGC GACGC(5/10) HincII 4262 GTTGAC GT[CT]^[GA]AC HindII 4262 GTTGAC GT[CT]^[GA]AC MamI 5076 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 583 CAATTG C^AATTG MunI 583 CAATTG C^AATTG NsiI 1364 ATGCAT ATGCA^T PflFI 1981 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 208 CACGTG CAC^GTG PmlI 208 CACGTG CAC^GTG PshAI 72 GACAGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 5306 CTGCAG CTGCA^G RcaI 3844 TCATGA T^CATGA Tth111I 1981 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5125 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflII 910 CTTAAG C^TTAAG 3996 CTTAAG ApaI 171 GGGCCC GGGCC^C 227 GGGCCC AspHI 414 GAGCTC G[AT]GC[AT]^C 4486 GAGCTC BbeI 41 GGCGCC GGCGC^C 281 GGCGCC BfrI 910 CTTAAG C^TTAAG 3996 CTTAAG BglII 3377 AGATCT A^GATCT 4462 AGATCT BsaAI 208 CACGTG [CT]AC^GT[GA] 2651 CACGTA BseRI 3271 CTCCTC GAGGAG(10/8) 4162 CTCCTC BsiHKAI 414 GAGCTC G[AT]GC[AT]^C 4486 GAGCTC BstEII 32 GGTGACC G^GT[ACGT]ACC 2871 GGTGACC BstPI 32 GGTGACC G^GT[ACGT]ACC 2871 GGTGACC DraIII 1212 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 1847 CACTTTGTG EcoO65I 32 GGTGACC G^GT[ACGT]ACC 2871 GGTGACC EcoT14I 1739 CCATGG C^C[AT][AT]GG 3976 CCATGG KasI 41 GGCGCC G^GCGCC 281 GGCGCC NarI 41 GGCGCC GG^CGCC 281 GGCGCC NcoI 1739 CCATGG C^CATGG 3976 CCATGG NdeI 1730 CATATG CA^TATG 3911 CATATG PpuMI 190 GGGACCC [GA]G^G[AT]CC[CT] 5629 GGGTCCC SacI 414 GAGCTC GAGCT^C 4486 GAGCTC SfiI 219 GGCCATCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 357 GGCCAGTCCGGCC SfoI 41 GGCGCC GGC^GCC 281 GGCGCC StuI 4761 AGGCCT AGG^CCT 4779 AGGCCT StyI 1739 CCATGG C^C[AT][AT]GG 3976 CCATGG TaqI 1185 TCGA T^CGA 1277 TCGA TthHB8I 1185 TCGA T^CGA 1277 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmnI