##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AviII AvrII BamHI BcgI
BciVI BlnI BlpI Bpu1102I BsaWI
BseAI BsiWI BsmBI BspEI BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I CelII CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HpaI KpnI KspI
MluI MroI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 715 GACGTC GACGT^C
AocI 4164 CCTCAGG CC^T[ACGT]AGG
AspI 1981 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 208 CACGTG CAC^GTG
BbvCI 5332 CCTCAGC CCTCAGC(-5/-2)
BsaBI 5076 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1735 GAGACC GGTCTC(1/5)
BsiEI 49 CGACCG CG[GA][CT]^CG
BsmI 804 GAATGC GAATGC(1/-1)
BspDI 1276 ATCGAT AT^CGAT
BspHI 3844 TCATGA T^CATGA
BsrFI 51 ACCGGC [GA]^CCGG[CT]
Bsu36I 4164 CCTCAGG CC^T[ACGT]AGG
Cfr10I 51 ACCGGC [GA]^CCGG[CT]
ClaI 1276 ATCGAT AT^CGAT
Eco81I 4164 CCTCAGG CC^T[ACGT]AGG
EcoNI 2137 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5319 GAATTC G^AATTC
EcoRV 3319 GATATC GAT^ATC
EcoT22I 1364 ATGCAT ATGCA^T
HgaI 307 GACGC GACGC(5/10)
HincII 4262 GTTGAC GT[CT]^[GA]AC
HindII 4262 GTTGAC GT[CT]^[GA]AC
MamI 5076 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 583 CAATTG C^AATTG
MunI 583 CAATTG C^AATTG
NsiI 1364 ATGCAT ATGCA^T
PflFI 1981 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 208 CACGTG CAC^GTG
PmlI 208 CACGTG CAC^GTG
PshAI 72 GACAGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 5306 CTGCAG CTGCA^G
RcaI 3844 TCATGA T^CATGA
Tth111I 1981 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5125 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 910 CTTAAG C^TTAAG
3996 CTTAAG
ApaI 171 GGGCCC GGGCC^C
227 GGGCCC
AspHI 414 GAGCTC G[AT]GC[AT]^C
4486 GAGCTC
BbeI 41 GGCGCC GGCGC^C
281 GGCGCC
BfrI 910 CTTAAG C^TTAAG
3996 CTTAAG
BglII 3377 AGATCT A^GATCT
4462 AGATCT
BsaAI 208 CACGTG [CT]AC^GT[GA]
2651 CACGTA
BseRI 3271 CTCCTC GAGGAG(10/8)
4162 CTCCTC
BsiHKAI 414 GAGCTC G[AT]GC[AT]^C
4486 GAGCTC
BstEII 32 GGTGACC G^GT[ACGT]ACC
2871 GGTGACC
BstPI 32 GGTGACC G^GT[ACGT]ACC
2871 GGTGACC
DraIII 1212 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1847 CACTTTGTG
EcoO65I 32 GGTGACC G^GT[ACGT]ACC
2871 GGTGACC
EcoT14I 1739 CCATGG C^C[AT][AT]GG
3976 CCATGG
KasI 41 GGCGCC G^GCGCC
281 GGCGCC
NarI 41 GGCGCC GG^CGCC
281 GGCGCC
NcoI 1739 CCATGG C^CATGG
3976 CCATGG
NdeI 1730 CATATG CA^TATG
3911 CATATG
PpuMI 190 GGGACCC [GA]G^G[AT]CC[CT]
5629 GGGTCCC
SacI 414 GAGCTC GAGCT^C
4486 GAGCTC
SfiI 219 GGCCATCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
357 GGCCAGTCCGGCC
SfoI 41 GGCGCC GGC^GCC
281 GGCGCC
StuI 4761 AGGCCT AGG^CCT
4779 AGGCCT
StyI 1739 CCATGG C^C[AT][AT]GG
3976 CCATGG
TaqI 1185 TCGA T^CGA
1277 TCGA
TthHB8I 1185 TCGA T^CGA
1277 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspBI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI