##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I ApaLI AscI Asp718I AviII
AvrII BamHI BbrPI BcgI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BsrFI BssHII
Bst1107I BstAPI BstBI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco52I EcoNI EcoT22I FseI
FspI HpaI KpnI KspI MamI
MluI MroI NaeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
ScaI SfuI SgrAI SnaBI SphI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1457 GTAGAC GT^[AC][GT]AC
AclI 4546 AACGTT AA^CGTT
AhdI 3299 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1703 CCTGAGG CC^T[ACGT]AGG
AseI 5532 ATTAAT AT^TAAT
AsnI 5532 ATTAAT AT^TAAT
AspEI 3299 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 5233 TGATCA T^GATCA
BlpI 3482 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3482 GCTGAGC GC^T[ACGT]AGC
BspHI 460 TCATGA T^CATGA
BssSI 5422 CTCGTG CACGAG(-5/-1)
BstEII 1148 GGTGACC G^GT[ACGT]ACC
BstPI 1148 GGTGACC G^GT[ACGT]ACC
Bsu36I 1703 CCTGAGG CC^T[ACGT]AGG
CelII 3482 GCTGAGC GC^T[ACGT]AGC
DraIII 3564 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3299 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1703 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1148 GGTGACC G^GT[ACGT]ACC
EcoRV 551 GATATC GAT^ATC
FbaI 5233 TGATCA T^GATCA
HincII 1525 GTCAAC GT[CT]^[GA]AC
HindII 1525 GTCAAC GT[CT]^[GA]AC
HindIII 2025 AAGCTT A^AGCTT
MfeI 4847 CAATTG C^AATTG
MunI 4847 CAATTG C^AATTG
NcoI 568 CCATGG C^CATGG
NdeI 5354 CATATG CA^TATG
NheI 3366 GCTAGC G^CTAGC
PshBI 5532 ATTAAT AT^TAAT
Psp1406I 4546 AACGTT AA^CGTT
RcaI 460 TCATGA T^CATGA
SfiI 2795 GGCCAAAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1821 CCCGGG CCC^GGG
SmlI 5378 CTTGAG C^T[CT][GA]AG
SpeI 3619 ACTAGT A^CTAGT
XbaI 4435 TCTAGA T^CTAGA
XmaCI 1821 CCCGGG C^CCGGG
XmaI 1821 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 4 CGCG CG^CG
1499 CGCG
Aor51HI 1395 AGCGCT AGC^GCT
1946 AGCGCT
ApaI 1490 GGGCCC GGGCC^C
5010 GGGCCC
Asp700I 3973 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5114 GAACTACTTC
AspI 2494 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2528 GACACTGTC
AvaI 1821 CCCGGG C^[CT]CG[GA]G
4055 CCCGAG
BbeI 795 GGCGCC GGCGC^C
4469 GGCGCC
BciVI 4491 GGATAC GTATCC(6/5)
3958 GTATCC
BglII 1113 AGATCT A^GATCT
1672 AGATCT
BsiEI 262 CGACCG CG[GA][CT]^CG
1691 CGGTCG
BsmBI 145 GAGACG CGTCTC(1/5)
258 GAGACG
BsmI 3763 GAATGC GAATGC(1/-1)
3120 GCATTC
BsoBI 1821 CCCGGG C^[CT]CG[GA]G
4055 CCCGAG
BstUI 4 CGCG CG^CG
1499 CGCG
BstXI 2545 CCACAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2584 CCACAACAATGG
Eco47III 1395 AGCGCT AGC^GCT
1946 AGCGCT
EcoRI 218 GAATTC G^AATTC
2848 GAATTC
KasI 795 GGCGCC G^GCGCC
4469 GGCGCC
MvnI 4 CGCG CG^CG
1499 CGCG
NarI 795 GGCGCC GG^CGCC
4469 GGCGCC
PflFI 2494 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2528 GACACTGTC
SexAI 1050 ACCAGGT A^CC[AT]GGT
4750 ACCTGGT
SfoI 795 GGCGCC GGC^GCC
4469 GGCGCC
TaqI 2063 TCGA T^CGA
5350 TCGA
Tth111I 2494 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2528 GACACTGTC
TthHB8I 2063 TCGA T^CGA
5350 TCGA
XcmI 1509 CCACAAATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3639 CCATCTAGGTGCTGG
XmnI 3973 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5114 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII