##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccI AccIII AcsI AflII
AgeI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
BamHI BciVI BfrI BglII BseAI
BsiWI BsmI BspEI BspHI BspLU11I
BssHII Bst1107I BstEII BstPI BstZ17I
CpoI DraI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI KpnI MfeI MluI MroI
MunI NdeI NotI NsiI PacI
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SalI SgrAI SpeI
SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1446 GACGTC GACGT^C
AclI 4268 AACGTT AA^CGTT
AhdI 3922 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4418 GGGCCC GGGCC^C
AspEI 3922 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4531 CCTAGG C^CTAGG
BbeI 3016 GGCGCC GGCGC^C
BclI 1418 TGATCA T^GATCA
BlnI 4531 CCTAGG C^CTAGG
BlpI 1046 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1046 GCTCAGC GC^T[ACGT]AGC
BsiEI 213 CGACCG CG[GA][CT]^CG
Bsp1407I 740 TGTACA T^GTACA
BsrDI 2198 GCAATG GCAATG(2/0)
BsrGI 740 TGTACA T^GTACA
BstBI 1309 TTCGAA TT^CGAA
CelII 1046 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3922 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1418 TGATCA T^GATCA
HindIII 1576 AAGCTT A^AGCTT
HpaI 4277 GTTAAC GTT^AAC
KasI 3016 GGCGCC G^GCGCC
NarI 3016 GGCGCC GG^CGCC
NheI 3678 GCTAGC G^CTAGC
NruI 2912 TCGCGA TCG^CGA
NspV 1309 TTCGAA TT^CGAA
PaeR7I 2762 CTCGAG C^TCGAG
Psp1406I 4268 AACGTT AA^CGTT
SbfI 1929 CCTGCAGG CCTGCA^GG
SexAI 1952 ACCTGGT A^CC[AT]GGT
SfiI 1614 GGCCTACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3016 GGCGCC GGC^GCC
SfuI 1309 TTCGAA TT^CGAA
SmaI 2516 CCCGGG CCC^GGG
SnaBI 669 TACGTA TAC^GTA
Sse8387I 1929 CCTGCAGG CCTGCA^GG
SspBI 740 TGTACA T^GTACA
XbaI 4260 TCTAGA T^CTAGA
XhoI 2762 CTCGAG C^TCGAG
XmaCI 2516 CCCGGG C^CCGGG
XmaI 2516 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 17 CCTCAGG CC^T[ACGT]AGG
3692 CCTCAGG
AviII 2560 TGCGCA TGC^GCA
3137 TGCGCA
BalI 371 TGGCCA TGG^CCA
2693 TGGCCA
BsaBI 1200 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2037 GATTGAAATC
BsaWI 2903 TCCGGT [AT]^CCGG[AT]
4516 ACCGGA
BsmBI 2382 CGTCTC CGTCTC(1/5)
2765 GAGACG
BspDI 2275 ATCGAT AT^CGAT
2479 ATCGAT
BsrBI 4351 CCGCTC CCGCTC(-3/-3)
3784 GAGCGG
BssSI 2935 CACGAG CACGAG(-5/-1)
2385 CTCGTG
BstAPI 1072 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3180 GCAGGTCCTGC
BstXI 2585 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3378 CCAGCGGAGTGG
Bsu36I 17 CCTCAGG CC^T[ACGT]AGG
3692 CCTCAGG
ClaI 2275 ATCGAT AT^CGAT
2479 ATCGAT
Eco81I 17 CCTCAGG CC^T[ACGT]AGG
3692 CCTCAGG
FspI 2560 TGCGCA TGC^GCA
3137 TGCGCA
MamI 1200 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2037 GATTGAAATC
MluNI 371 TGGCCA TGG^CCA
2693 TGGCCA
MscI 371 TGGCCA TGG^CCA
2693 TGGCCA
NaeI 887 GCCGGC GCC^GGC
3701 GCCGGC
NcoI 811 CCATGG C^CATGG
4307 CCATGG
NgoMIV 887 GCCGGC G^CCGGC
3701 GCCGGC
PflMI 3216 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4302 CCACACCATGG
ScaI 383 AGTACT AGT^ACT
2555 AGTACT
SphI 4467 GCATGC GCATG^C
4708 GCATGC
StuI 3644 AGGCCT AGG^CCT
4555 AGGCCT
Van91I 3216 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4302 CCACACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AspI
AvaI AvaII BanI BanII BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII