################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AflII AgeI Aor51HI AscI AviII AvrII BbeI BfrI BlnI BsaBI BsiWI BspDI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI Eco47III EcoRV FseI FspI KasI KspI MamI MluI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PinAI PmeI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 475 GGTACC G^GTACC AclI 5748 AACGTT AA^CGTT AhdI 3465 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 475 GGTACC G^GTACC AspEI 3465 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 3612 CACGTG CAC^GTG BcgI 4846 CGACTGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2790 GGATAC GTATCC(6/5) BsaAI 3612 CACGTG [CT]AC^GT[GA] BsiEI 3602 CGGCCG CG[GA][CT]^CG Bsp1407I 6546 TGTACA T^GTACA BspHI 3361 TCATGA T^CATGA BsrBI 3711 GAGCGG CCGCTC(-3/-3) BsrGI 6546 TGTACA T^GTACA DrdI 5318 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3602 CGGCCG C^GGCCG Eam1105I 3465 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3602 CGGCCG C^GGCCG Eco52I 3602 CGGCCG C^GGCCG EcoRI 5774 GAATTC G^AATTC EcoT22I 2084 ATGCAT ATGCA^T HpaI 1716 GTTAAC GTT^AAC KpnI 475 GGTACC GGTAC^C MfeI 267 CAATTG C^AATTG MunI 267 CAATTG C^AATTG NcoI 344 CCATGG C^CATGG NheI 1298 GCTAGC G^CTAGC NsiI 2084 ATGCAT ATGCA^T PacI 5398 TTAATTAA TTAAT^TAA PmaCI 3612 CACGTG CAC^GTG PmlI 3612 CACGTG CAC^GTG Psp1406I 5748 AACGTT AA^CGTT RcaI 3361 TCATGA T^CATGA ScaI 5277 AGTACT AGT^ACT SpeI 1193 ACTAGT A^CTAGT SspBI 6546 TGTACA T^GTACA SspI 6584 AATATT AAT^ATT XbaI 6146 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccIII 3326 TCCGGA T^CCGGA 4327 TCCGGA AflIII 5556 ACATGT A^C[GA][CT]GT 6285 ACATGT Alw44I 1607 GTGCAC G^TGCAC 5663 GTGCAC AocI 2495 CCTCAGG CC^T[ACGT]AGG 5850 CCTTAGG ApaI 877 GGGCCC GGGCC^C 4031 GGGCCC ApaLI 1607 GTGCAC G^TGCAC 5663 GTGCAC BamHI 2911 GGATCC G^GATCC 4330 GGATCC BanI 475 GGTACC G^G[CT][GA]CC 1152 GGCACC BbsI 2777 GAAGAC GAAGAC(2/6) 4494 GAAGAC BbvCI 3791 CCTCAGC CCTCAGC(-5/-2) 2867 GCTGAGG BclI 905 TGATCA T^GATCA 1942 TGATCA BpuAI 2777 GAAGAC GAAGAC(2/6) 4494 GAAGAC BseAI 3326 TCCGGA T^CCGGA 4327 TCCGGA BsmBI 1055 GAGACG CGTCTC(1/5) 2670 GAGACG BsmI 581 GAATGC GAATGC(1/-1) 4922 GAATGC BspEI 3326 TCCGGA T^CCGGA 4327 TCCGGA BspLU11I 5556 ACATGT A^CATGT 6285 ACATGT BsrDI 3025 CATTGC GCAATG(2/0) 5966 CATTGC Bsu36I 2495 CCTCAGG CC^T[ACGT]AGG 5850 CCTTAGG DraIII 312 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1533 CACCCCGTG DsaI 344 CCATGG C^C[GA][CT]GG 2958 CCGTGG Eco81I 2495 CCTCAGG CC^T[ACGT]AGG 5850 CCTTAGG EcoNI 533 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1061 CCTGACGGAGG FbaI 905 TGATCA T^GATCA 1942 TGATCA HaeII 2050 AGCGCC [GA]GCGC^[CT] 5867 GGCGCT MroI 3326 TCCGGA T^CCGGA 4327 TCCGGA NdeI 389 CATATG CA^TATG 5889 CATATG PshAI 603 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 6055 GACTTCTGTC SmlI 99 CTTGAG C^T[CT][GA]AG 1407 CTTGAG SphI 1928 GCATGC GCATG^C 4619 GCATGC StuI 1542 AGGCCT AGG^CCT 3208 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AspI AvaI AvaII BalI BanII BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PshBI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI