##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AatII       AflII       AgeI        Aor51HI     AscI        
AviII       AvrII       BbeI        BfrI        BlnI        
BsaBI       BsiWI       BspDI       BsrFI       BssHII      
Bst1107I    BstBI       BstZ17I     Cfr10I      ClaI        
CpoI        Eco47III    EcoRV       FseI        FspI        
KasI        KspI        MamI        MluI        NaeI        
NarI        NgoMIV      NotI        NruI        NspV        
PaeR7I      PinAI       PmeI        PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        475  GGTACC               G^GTACC
AclI         5748  AACGTT               AA^CGTT
AhdI         3465  GACCTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I       475  GGTACC               G^GTACC
AspEI        3465  GACCTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        3612  CACGTG               CAC^GTG
BcgI         4846  CGACTGAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2790  GGATAC               GTATCC(6/5)
BsaAI        3612  CACGTG               [CT]AC^GT[GA]
BsiEI        3602  CGGCCG               CG[GA][CT]^CG
Bsp1407I     6546  TGTACA               T^GTACA
BspHI        3361  TCATGA               T^CATGA
BsrBI        3711  GAGCGG               CCGCTC(-3/-3)
BsrGI        6546  TGTACA               T^GTACA
DrdI         5318  GACAGCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3602  CGGCCG               C^GGCCG
Eam1105I     3465  GACCTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3602  CGGCCG               C^GGCCG
Eco52I       3602  CGGCCG               C^GGCCG
EcoRI        5774  GAATTC               G^AATTC
EcoT22I      2084  ATGCAT               ATGCA^T
HpaI         1716  GTTAAC               GTT^AAC
KpnI          475  GGTACC               GGTAC^C
MfeI          267  CAATTG               C^AATTG
MunI          267  CAATTG               C^AATTG
NcoI          344  CCATGG               C^CATGG
NheI         1298  GCTAGC               G^CTAGC
NsiI         2084  ATGCAT               ATGCA^T
PacI         5398  TTAATTAA             TTAAT^TAA
PmaCI        3612  CACGTG               CAC^GTG
PmlI         3612  CACGTG               CAC^GTG
Psp1406I     5748  AACGTT               AA^CGTT
RcaI         3361  TCATGA               T^CATGA
ScaI         5277  AGTACT               AGT^ACT
SpeI         1193  ACTAGT               A^CTAGT
SspBI        6546  TGTACA               T^GTACA
SspI         6584  AATATT               AAT^ATT
XbaI         6146  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       3326  TCCGGA               T^CCGGA
             4327  TCCGGA              
AflIII       5556  ACATGT               A^C[GA][CT]GT
             6285  ACATGT              
Alw44I       1607  GTGCAC               G^TGCAC
             5663  GTGCAC              
AocI         2495  CCTCAGG              CC^T[ACGT]AGG
             5850  CCTTAGG             
ApaI          877  GGGCCC               GGGCC^C
             4031  GGGCCC              
ApaLI        1607  GTGCAC               G^TGCAC
             5663  GTGCAC              
BamHI        2911  GGATCC               G^GATCC
             4330  GGATCC              
BanI          475  GGTACC               G^G[CT][GA]CC
             1152  GGCACC              
BbsI         2777  GAAGAC               GAAGAC(2/6)
             4494  GAAGAC              
BbvCI        3791  CCTCAGC              CCTCAGC(-5/-2)
             2867  GCTGAGG             
BclI          905  TGATCA               T^GATCA
             1942  TGATCA              
BpuAI        2777  GAAGAC               GAAGAC(2/6)
             4494  GAAGAC              
BseAI        3326  TCCGGA               T^CCGGA
             4327  TCCGGA              
BsmBI        1055  GAGACG               CGTCTC(1/5)
             2670  GAGACG              
BsmI          581  GAATGC               GAATGC(1/-1)
             4922  GAATGC              
BspEI        3326  TCCGGA               T^CCGGA
             4327  TCCGGA              
BspLU11I     5556  ACATGT               A^CATGT
             6285  ACATGT              
BsrDI        3025  CATTGC               GCAATG(2/0)
             5966  CATTGC              
Bsu36I       2495  CCTCAGG              CC^T[ACGT]AGG
             5850  CCTTAGG             
DraIII        312  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1533  CACCCCGTG           
DsaI          344  CCATGG               C^C[GA][CT]GG
             2958  CCGTGG              
Eco81I       2495  CCTCAGG              CC^T[ACGT]AGG
             5850  CCTTAGG             
EcoNI         533  CCTGATGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1061  CCTGACGGAGG         
FbaI          905  TGATCA               T^GATCA
             1942  TGATCA              
HaeII        2050  AGCGCC               [GA]GCGC^[CT]
             5867  GGCGCT              
MroI         3326  TCCGGA               T^CCGGA
             4327  TCCGGA              
NdeI          389  CATATG               CA^TATG
             5889  CATATG              
PshAI         603  GACCGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             6055  GACTTCTGTC          
SmlI           99  CTTGAG               C^T[CT][GA]AG
             1407  CTTGAG              
SphI         1928  GCATGC               GCATG^C
             4619  GCATGC              
StuI         1542  AGGCCT               AGG^CCT
             3208  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApoI        
AseI        AsnI        Asp700I     AspHI       AspI        
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BfaI        BglI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BssSI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PpuMI       PshBI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XcmI        XhoII       XmnI