##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AflII AgeI Aor51HI AscI
AviII AvrII BbeI BfrI BlnI
BsaBI BsiWI BspDI BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI Eco47III EcoRV FseI FspI
KasI KspI MamI MluI NaeI
NarI NgoMIV NotI NruI NspV
PaeR7I PinAI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 475 GGTACC G^GTACC
AclI 5748 AACGTT AA^CGTT
AhdI 3465 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 475 GGTACC G^GTACC
AspEI 3465 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3612 CACGTG CAC^GTG
BcgI 4846 CGACTGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2790 GGATAC GTATCC(6/5)
BsaAI 3612 CACGTG [CT]AC^GT[GA]
BsiEI 3602 CGGCCG CG[GA][CT]^CG
Bsp1407I 6546 TGTACA T^GTACA
BspHI 3361 TCATGA T^CATGA
BsrBI 3711 GAGCGG CCGCTC(-3/-3)
BsrGI 6546 TGTACA T^GTACA
DrdI 5318 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3602 CGGCCG C^GGCCG
Eam1105I 3465 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3602 CGGCCG C^GGCCG
Eco52I 3602 CGGCCG C^GGCCG
EcoRI 5774 GAATTC G^AATTC
EcoT22I 2084 ATGCAT ATGCA^T
HpaI 1716 GTTAAC GTT^AAC
KpnI 475 GGTACC GGTAC^C
MfeI 267 CAATTG C^AATTG
MunI 267 CAATTG C^AATTG
NcoI 344 CCATGG C^CATGG
NheI 1298 GCTAGC G^CTAGC
NsiI 2084 ATGCAT ATGCA^T
PacI 5398 TTAATTAA TTAAT^TAA
PmaCI 3612 CACGTG CAC^GTG
PmlI 3612 CACGTG CAC^GTG
Psp1406I 5748 AACGTT AA^CGTT
RcaI 3361 TCATGA T^CATGA
ScaI 5277 AGTACT AGT^ACT
SpeI 1193 ACTAGT A^CTAGT
SspBI 6546 TGTACA T^GTACA
SspI 6584 AATATT AAT^ATT
XbaI 6146 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 3326 TCCGGA T^CCGGA
4327 TCCGGA
AflIII 5556 ACATGT A^C[GA][CT]GT
6285 ACATGT
Alw44I 1607 GTGCAC G^TGCAC
5663 GTGCAC
AocI 2495 CCTCAGG CC^T[ACGT]AGG
5850 CCTTAGG
ApaI 877 GGGCCC GGGCC^C
4031 GGGCCC
ApaLI 1607 GTGCAC G^TGCAC
5663 GTGCAC
BamHI 2911 GGATCC G^GATCC
4330 GGATCC
BanI 475 GGTACC G^G[CT][GA]CC
1152 GGCACC
BbsI 2777 GAAGAC GAAGAC(2/6)
4494 GAAGAC
BbvCI 3791 CCTCAGC CCTCAGC(-5/-2)
2867 GCTGAGG
BclI 905 TGATCA T^GATCA
1942 TGATCA
BpuAI 2777 GAAGAC GAAGAC(2/6)
4494 GAAGAC
BseAI 3326 TCCGGA T^CCGGA
4327 TCCGGA
BsmBI 1055 GAGACG CGTCTC(1/5)
2670 GAGACG
BsmI 581 GAATGC GAATGC(1/-1)
4922 GAATGC
BspEI 3326 TCCGGA T^CCGGA
4327 TCCGGA
BspLU11I 5556 ACATGT A^CATGT
6285 ACATGT
BsrDI 3025 CATTGC GCAATG(2/0)
5966 CATTGC
Bsu36I 2495 CCTCAGG CC^T[ACGT]AGG
5850 CCTTAGG
DraIII 312 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1533 CACCCCGTG
DsaI 344 CCATGG C^C[GA][CT]GG
2958 CCGTGG
Eco81I 2495 CCTCAGG CC^T[ACGT]AGG
5850 CCTTAGG
EcoNI 533 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1061 CCTGACGGAGG
FbaI 905 TGATCA T^GATCA
1942 TGATCA
HaeII 2050 AGCGCC [GA]GCGC^[CT]
5867 GGCGCT
MroI 3326 TCCGGA T^CCGGA
4327 TCCGGA
NdeI 389 CATATG CA^TATG
5889 CATATG
PshAI 603 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6055 GACTTCTGTC
SmlI 99 CTTGAG C^T[CT][GA]AG
1407 CTTGAG
SphI 1928 GCATGC GCATG^C
4619 GCATGC
StuI 1542 AGGCCT AGG^CCT
3208 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AspI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PshBI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI