##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AclI AscI AseI AsnI
Asp718I AspI AvrII BbrPI BcgI
BciVI BlnI BsaAI BsiWI BsmI
Bsp1407I BspDI BspHI BsrGI BssHII
Bst1107I BstZ17I ClaI DraI EagI
EclXI Eco52I EcoT22I FseI HpaI
KpnI KspI MfeI MluI MunI
NcoI NheI NotI NruI NsiI
PacI PflFI PmaCI PmeI PmlI
PshBI Psp1406I RcaI SacII SalI
SbfI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 2945 GACGTC GACGT^C
AccI 5048 GTAGAC GT^[AC][GT]AC
AgeI 3579 ACCGGT A^CCGGT
Alw44I 4845 GTGCAC G^TGCAC
ApaLI 4845 GTGCAC G^TGCAC
AviII 5974 TGCGCA TGC^GCA
BbeI 5797 GGCGCC GGCGC^C
BclI 2625 TGATCA T^GATCA
BsiEI 5061 CGATCG CG[GA][CT]^CG
BsmBI 1605 CGTCTC CGTCTC(1/5)
BssSI 4512 CACGAG CACGAG(-5/-1)
BstEII 2473 GGTAACC G^GT[ACGT]ACC
BstPI 2473 GGTAACC G^GT[ACGT]ACC
CpoI 1103 CGGACCG CG^G[AT]CCG
DrdI 3589 GACCCGTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2473 GGTAACC G^GT[ACGT]ACC
FbaI 2625 TGATCA T^GATCA
FspI 5974 TGCGCA TGC^GCA
HindIII 2126 AAGCTT A^AGCTT
KasI 5797 GGCGCC G^GCGCC
NarI 5797 GGCGCC GG^CGCC
PinAI 3579 ACCGGT A^CCGGT
PshAI 2344 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 5061 CGATCG CGAT^CG
RsrII 1103 CGGACCG CG^G[AT]CCG
SexAI 3075 ACCAGGT A^CC[AT]GGT
SfoI 5797 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1101 TCCGGA T^CCGGA
2164 TCCGGA
Aor51HI 1893 AGCGCT AGC^GCT
2405 AGCGCT
ApaI 5 GGGCCC GGGCC^C
6245 GGGCCC
Asp700I 2795 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6166 GAATTTCTTC
BalI 2640 TGGCCA TGG^CCA
3468 TGGCCA
BamHI 1317 GGATCC G^GATCC
3991 GGATCC
BbvCI 4742 GCTGAGG CCTCAGC(-5/-2)
5567 GCTGAGG
BlpI 96 GCTCAGC GC^T[ACGT]AGC
4925 GCTGAGC
Bpu1102I 96 GCTCAGC GC^T[ACGT]AGC
4925 GCTGAGC
BseAI 1101 TCCGGA T^CCGGA
2164 TCCGGA
BspEI 1101 TCCGGA T^CCGGA
2164 TCCGGA
BsrBI 5246 GAGCGG CCGCTC(-3/-3)
5765 GAGCGG
BstBI 3044 TTCGAA TT^CGAA
4569 TTCGAA
CelII 96 GCTCAGC GC^T[ACGT]AGC
4925 GCTGAGC
Eco47III 1893 AGCGCT AGC^GCT
2405 AGCGCT
EcoRI 695 GAATTC G^AATTC
1412 GAATTC
EcoRV 3713 GATATC GAT^ATC
5852 GATATC
HincII 4091 GTCAAC GT[CT]^[GA]AC
4530 GTCAAC
HindII 4091 GTCAAC GT[CT]^[GA]AC
4530 GTCAAC
MluNI 2640 TGGCCA TGG^CCA
3468 TGGCCA
MroI 1101 TCCGGA T^CCGGA
2164 TCCGGA
MscI 2640 TGGCCA TGG^CCA
3468 TGGCCA
NaeI 300 GCCGGC GCC^GGC
5632 GCCGGC
NdeI 1241 CATATG CA^TATG
2921 CATATG
NgoMIV 300 GCCGGC G^CCGGC
5632 GCCGGC
NspV 3044 TTCGAA TT^CGAA
4569 TTCGAA
PstI 1745 CTGCAG CTGCA^G
3227 CTGCAG
SacI 1442 GAGCTC GAGCT^C
3895 GAGCTC
ScaI 3015 AGTACT AGT^ACT
4300 AGTACT
SfcI 1745 CTGCAG C^T[GA][CT]AG
3227 CTGCAG
SfuI 3044 TTCGAA TT^CGAA
4569 TTCGAA
SphI 4359 GCATGC GCATG^C
4614 GCATGC
SspI 3888 AATATT AAT^ATT
4292 AATATT
XbaI 2788 TCTAGA T^CTAGA
5422 TCTAGA
XmnI 2795 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6166 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AhdI AluI AlwI
AlwNI AocI ApoI AspEI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BfrI BglI
BglII BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII