##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AviII BfrI BglII BsaWI
BseAI BsiWI BsmBI BspDI BspEI
Bst1107I BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
EcoRV FseI FspI HincII HindII
HpaI KpnI MluI MroI NdeI
NheI NotI NruI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI ScaI SexAI SfiI
SnaBI SpeI SphI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 636 GTCTAC GT^[AC][GT]AC
AflIII 1052 ACATGT A^C[GA][CT]GT
BamHI 2250 GGATCC G^GATCC
BbeI 3275 GGCGCC GGCGC^C
BbrPI 3776 CACGTG CAC^GTG
BbvCI 4106 CCTCAGC CCTCAGC(-5/-2)
BcgI 3901 GCAGTGTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5184 GTATCC GTATCC(6/5)
BclI 1553 TGATCA T^GATCA
BsiEI 2340 CGACCG CG[GA][CT]^CG
Bsp1407I 881 TGTACA T^GTACA
BspHI 1730 TCATGA T^CATGA
BspLU11I 1052 ACATGT A^CATGT
BsrGI 881 TGTACA T^GTACA
BssHII 4584 GCGCGC G^CGCGC
BstAPI 4404 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3113 TTCGAA TT^CGAA
EcoNI 1380 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 610 GAATTC G^AATTC
FbaI 1553 TGATCA T^GATCA
HindIII 1627 AAGCTT A^AGCTT
KasI 3275 GGCGCC G^GCGCC
KspI 4222 CCGCGG CCGC^GG
MfeI 5283 CAATTG C^AATTG
MunI 5283 CAATTG C^AATTG
NarI 3275 GGCGCC GG^CGCC
NspV 3113 TTCGAA TT^CGAA
PaeR7I 3224 CTCGAG C^TCGAG
PmaCI 3776 CACGTG CAC^GTG
PmlI 3776 CACGTG CAC^GTG
RcaI 1730 TCATGA T^CATGA
SacI 3883 GAGCTC GAGCT^C
SacII 4222 CCGCGG CCGC^GG
SfoI 3275 GGCGCC GGC^GCC
SfuI 3113 TTCGAA TT^CGAA
SgrAI 918 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 881 TGTACA T^GTACA
XhoI 3224 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 610 GAATTC [GA]^AATT[CT]
5292 AAATTT
AhdI 2673 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4486 GACATCACGTC
Aor51HI 248 AGCGCT AGC^GCT
713 AGCGCT
ApoI 610 GAATTC [GA]^AATT[CT]
5292 AAATTT
AspEI 2673 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4486 GACATCACGTC
AvrII 3265 CCTAGG C^CTAGG
4383 CCTAGG
BlnI 3265 CCTAGG C^CTAGG
4383 CCTAGG
BlpI 30 GCTTAGC GC^T[ACGT]AGC
4100 GCTTAGC
Bpu1102I 30 GCTTAGC GC^T[ACGT]AGC
4100 GCTTAGC
BsaBI 192 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
580 GATGACCATC
BsgI 4187 CTGCAC GTGCAG(16/14)
3534 GTGCAG
BsmI 5192 GAATGC GAATGC(1/-1)
4058 GCATTC
BsrDI 1527 CATTGC GCAATG(2/0)
3549 CATTGC
BssSI 1871 CACGAG CACGAG(-5/-1)
1015 CTCGTG
BstEII 360 GGTCACC G^GT[ACGT]ACC
1021 GGTGACC
BstPI 360 GGTCACC G^GT[ACGT]ACC
1021 GGTGACC
CelII 30 GCTTAGC GC^T[ACGT]AGC
4100 GCTTAGC
Eam1105I 2673 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4486 GACATCACGTC
Eco47III 248 AGCGCT AGC^GCT
713 AGCGCT
EcoO65I 360 GGTCACC G^GT[ACGT]ACC
1021 GGTGACC
EcoT22I 54 ATGCAT ATGCA^T
3063 ATGCAT
MamI 192 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
580 GATGACCATC
MseI 5046 TTAA T^TAA
5067 TTAA
NaeI 227 GCCGGC GCC^GGC
1075 GCCGGC
NcoI 2 CCATGG C^CATGG
297 CCATGG
NgoMIV 227 GCCGGC G^CCGGC
1075 GCCGGC
NsiI 54 ATGCAT ATGCA^T
3063 ATGCAT
PflMI 1917 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3748 CCACACAATGG
SapI 7 GAAGAGC GCTCTTC(1/4)
3730 GAAGAGC
Tru9I 5046 TTAA T^TAA
5067 TTAA
Van91I 1917 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3748 CCACACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmaI SmlI Sse8387I StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII
XmaCI XmaI