##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstPI Cfr10I ClaI
CpoI DraII EagI EclXI Eco52I
EcoO109I EcoO65I FauI FseI FspI
KasI MamI MluI MluNI MroI
MscI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 841 CGCG CG^CG
AcyI 385 GGCGTC G[GA]^CG[CT]C
AflII 5877 CTTAAG C^TTAAG
AhdI 118 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 371 GTGCAC G^TGCAC
Aor51HI 1299 AGCGCT AGC^GCT
ApaLI 371 GTGCAC G^TGCAC
AspEI 118 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1520 CCCGAG C^[CT]CG[GA]G
BcgI 4670 GCAGAAGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4368 GGATAC GTATCC(6/5)
BclI 1869 TGATCA T^GATCA
BfrI 5877 CTTAAG C^TTAAG
BsaHI 385 GGCGTC G[GA]^CG[CT]C
BsoBI 1520 CCCGAG C^[CT]CG[GA]G
BspHI 166 TCATGA T^CATGA
BsrDI 4881 CATTGC GCAATG(2/0)
BssSI 1388 CACGAG CACGAG(-5/-1)
BstUI 841 CGCG CG^CG
DraIII 2836 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 843 CGGCCA [CT]^GGCC[GA]
Eam1105I 118 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1299 AGCGCT AGC^GCT
EcoRV 4824 GATATC GAT^ATC
FbaI 1869 TGATCA T^GATCA
HaeII 1299 AGCGCT [GA]GCGC^[CT]
Hin1I 385 GGCGTC G[GA]^CG[CT]C
KspI 840 CCGCGG CCGC^GG
MfeI 5837 CAATTG C^AATTG
MunI 5837 CAATTG C^AATTG
MvnI 841 CGCG CG^CG
NheI 3617 GCTAGC G^CTAGC
PflMI 376 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 424 GTTTAAAC GTTT^AAAC
RcaI 166 TCATGA T^CATGA
SacII 840 CCGCGG CCGC^GG
SapI 5947 GAAGAGC GCTCTTC(1/4)
SpeI 2990 ACTAGT A^CTAGT
StuI 3188 AGGCCT AGG^CCT
Van91I 376 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 1076 GATCC GGATC(4/5)
1517 GATCC
AseI 3469 ATTAAT AT^TAAT
4290 ATTAAT
AsnI 3469 ATTAAT AT^TAAT
4290 ATTAAT
AvaII 3295 GGTCC G^G[AT]CC
4137 GGACC
BglII 885 AGATCT A^GATCT
3215 AGATCT
BlpI 3313 GCTAAGC GC^T[ACGT]AGC
4355 GCTTAGC
Bpu1102I 3313 GCTAAGC GC^T[ACGT]AGC
4355 GCTTAGC
BsaI 1407 GAGACC GGTCTC(1/5)
4232 GAGACC
BsgI 531 CTGCAC GTGCAG(16/14)
4776 CTGCAC
BspLU11I 1232 ACATGT A^CATGT
2815 ACATGT
Bst1107I 185 GTATAC GTA^TAC
1988 GTATAC
BstZ17I 185 GTATAC GTA^TAC
1988 GTATAC
CelII 3313 GCTAAGC GC^T[ACGT]AGC
4355 GCTTAGC
CfoI 766 GCGC GCG^C
1300 GCGC
EcoT22I 738 ATGCAT ATGCA^T
4530 ATGCAT
HgaI 386 GCGTC GACGC(5/10)
1911 GCGTC
HhaI 766 GCGC GCG^C
1300 GCGC
HinP1I 766 GCGC G^CGC
1300 GCGC
HindIII 32 AAGCTT A^AGCTT
5394 AAGCTT
HpaI 2614 GTTAAC GTT^AAC
2811 GTTAAC
NcoI 1620 CCATGG C^CATGG
3365 CCATGG
NdeI 3442 CATATG CA^TATG
5090 CATATG
NsiI 738 ATGCAT ATGCA^T
4530 ATGCAT
PshBI 3469 ATTAAT AT^TAAT
4290 ATTAAT
PstI 2912 CTGCAG CTGCA^G
4511 CTGCAG
SacI 665 GAGCTC GAGCT^C
3344 GAGCTC
TaqI 1310 TCGA T^CGA
2260 TCGA
TthHB8I 1310 TCGA T^CGA
2260 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflIII AluI AlwNI AocI ApoI
Asp700I Asp718I AspHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DrdI DsaI EarI Eco81I EcoNI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI XhoII XmnI