################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AatII AgeI AscI AviII BamHI BbeI BbrPI BbvCI BcgI BsaBI BsiEI BsiWI BspLU11I BssHII Bst1107I BstBI BstZ17I EagI EclXI Eco52I EcoRI FseI FspI HpaI KasI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PvuI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 3870 GGTACC G^GTACC AccIII 5644 TCCGGA T^CCGGA AcyI 796 GACGCC G[GA]^CG[CT]C AflII 3291 CTTAAG C^TTAAG AflIII 554 ACACGT A^C[GA][CT]GT AlwNI 523 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 829 CCTGAGG CC^T[ACGT]AGG AseI 726 ATTAAT AT^TAAT AsnI 726 ATTAAT AT^TAAT Asp718I 3870 GGTACC G^GTACC AspI 1492 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 2166 GGATAC GTATCC(6/5) BfrI 3291 CTTAAG C^TTAAG BsaAI 4079 CACGTA [CT]AC^GT[GA] BsaHI 796 GACGCC G[GA]^CG[CT]C BsaWI 5644 TCCGGA [AT]^CCGG[AT] BseAI 5644 TCCGGA T^CCGGA BspEI 5644 TCCGGA T^CCGGA BspHI 264 TCATGA T^CATGA BsrBI 622 CCGCTC CCGCTC(-3/-3) BsrDI 6048 CATTGC GCAATG(2/0) BsrFI 1136 GCCGGT [GA]^CCGG[CT] BssSI 3268 CTCGTG CACGAG(-5/-1) BstEII 5316 GGTGACC G^GT[ACGT]ACC BstPI 5316 GGTGACC G^GT[ACGT]ACC Bsu36I 829 CCTGAGG CC^T[ACGT]AGG Cfr10I 1136 GCCGGT [GA]^CCGG[CT] CpoI 3232 CGGTCCG CG^G[AT]CCG DrdI 3850 GACCCCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 829 CCTGAGG CC^T[ACGT]AGG EcoO65I 5316 GGTGACC G^GT[ACGT]ACC EcoRV 940 GATATC GAT^ATC Hin1I 796 GACGCC G[GA]^CG[CT]C KpnI 3870 GGTACC GGTAC^C MroI 5644 TCCGGA T^CCGGA NcoI 1069 CCATGG C^CATGG PaeR7I 897 CTCGAG C^TCGAG PflFI 1492 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 726 ATTAAT AT^TAAT RcaI 264 TCATGA T^CATGA RsrII 3232 CGGTCCG CG^G[AT]CCG SapI 2229 GAAGAGC GCTCTTC(1/4) ScaI 1391 AGTACT AGT^ACT SpeI 4691 ACTAGT A^CTAGT SwaI 4504 ATTTAAAT ATTT^AAAT Tth111I 1492 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 897 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccII 1045 CGCG CG^CG 3171 CGCG AclI 4946 AACGTT AA^CGTT 6302 AACGTT AhdI 1865 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2005 GACAAATTGTC Alw44I 5252 GTGCAC G^TGCAC 5627 GTGCAC ApaI 2599 GGGCCC GGGCC^C 3583 GGGCCC ApaLI 5252 GTGCAC G^TGCAC 5627 GTGCAC Asp700I 1 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3884 GAATACCTTC AspEI 1865 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2005 GACAAATTGTC BclI 4110 TGATCA T^GATCA 6149 TGATCA BglI 1010 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3189 GCCGTCAGGGC BglII 12 AGATCT A^GATCT 1289 AGATCT BlpI 624 GCTCAGC GC^T[ACGT]AGC 1540 GCTAAGC Bpu1102I 624 GCTCAGC GC^T[ACGT]AGC 1540 GCTAAGC Bsp1407I 6090 TGTACA T^GTACA 6288 TGTACA BspDI 1773 ATCGAT AT^CGAT 5732 ATCGAT BsrGI 6090 TGTACA T^GTACA 6288 TGTACA BstAPI 1343 GCAACGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3180 GCAGGATTTGC BstUI 1045 CGCG CG^CG 3171 CGCG BstXI 2745 CCAGGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4590 CCACAGTATTGG CelII 624 GCTCAGC GC^T[ACGT]AGC 1540 GCTAAGC ClaI 1773 ATCGAT AT^CGAT 5732 ATCGAT DraIII 848 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 907 CACAAAGTG DsaI 293 CCACGG C^C[GA][CT]GG 1069 CCATGG Eam1105I 1865 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2005 GACAAATTGTC EcoNI 282 CCTTGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1423 CCTCGACCAGG EcoT22I 4041 ATGCAT ATGCA^T 4500 ATGCAT FbaI 4110 TGATCA T^GATCA 6149 TGATCA HindIII 658 AAGCTT A^AGCTT 5760 AAGCTT MvnI 1045 CGCG CG^CG 3171 CGCG NheI 2248 GCTAGC G^CTAGC 3198 GCTAGC NsiI 4041 ATGCAT ATGCA^T 4500 ATGCAT PshAI 694 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 878 GACGGAAGTC Psp1406I 4946 AACGTT AA^CGTT 6302 AACGTT SacI 5070 GAGCTC GAGCT^C 5828 GAGCTC SexAI 1219 ACCAGGT A^CC[AT]GGT 2564 ACCTGGT SmaI 1399 CCCGGG CCC^GGG 2410 CCCGGG SspBI 6090 TGTACA T^GTACA 6288 TGTACA SspI 1940 AATATT AAT^ATT 2627 AATATT XmaCI 1399 CCCGGG C^CCGGG 2410 CCCGGG XmaI 1399 CCCGGG C^CCGGG 2410 CCCGGG XmnI 1 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3884 GAATACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI Aor51HI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII