##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AgeI AscI AviII BamHI
BbeI BbrPI BbvCI BcgI BsaBI
BsiEI BsiWI BspLU11I BssHII Bst1107I
BstBI BstZ17I EagI EclXI Eco52I
EcoRI FseI FspI HpaI KasI
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PvuI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3870 GGTACC G^GTACC
AccIII 5644 TCCGGA T^CCGGA
AcyI 796 GACGCC G[GA]^CG[CT]C
AflII 3291 CTTAAG C^TTAAG
AflIII 554 ACACGT A^C[GA][CT]GT
AlwNI 523 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 829 CCTGAGG CC^T[ACGT]AGG
AseI 726 ATTAAT AT^TAAT
AsnI 726 ATTAAT AT^TAAT
Asp718I 3870 GGTACC G^GTACC
AspI 1492 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2166 GGATAC GTATCC(6/5)
BfrI 3291 CTTAAG C^TTAAG
BsaAI 4079 CACGTA [CT]AC^GT[GA]
BsaHI 796 GACGCC G[GA]^CG[CT]C
BsaWI 5644 TCCGGA [AT]^CCGG[AT]
BseAI 5644 TCCGGA T^CCGGA
BspEI 5644 TCCGGA T^CCGGA
BspHI 264 TCATGA T^CATGA
BsrBI 622 CCGCTC CCGCTC(-3/-3)
BsrDI 6048 CATTGC GCAATG(2/0)
BsrFI 1136 GCCGGT [GA]^CCGG[CT]
BssSI 3268 CTCGTG CACGAG(-5/-1)
BstEII 5316 GGTGACC G^GT[ACGT]ACC
BstPI 5316 GGTGACC G^GT[ACGT]ACC
Bsu36I 829 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1136 GCCGGT [GA]^CCGG[CT]
CpoI 3232 CGGTCCG CG^G[AT]CCG
DrdI 3850 GACCCCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 829 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5316 GGTGACC G^GT[ACGT]ACC
EcoRV 940 GATATC GAT^ATC
Hin1I 796 GACGCC G[GA]^CG[CT]C
KpnI 3870 GGTACC GGTAC^C
MroI 5644 TCCGGA T^CCGGA
NcoI 1069 CCATGG C^CATGG
PaeR7I 897 CTCGAG C^TCGAG
PflFI 1492 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 726 ATTAAT AT^TAAT
RcaI 264 TCATGA T^CATGA
RsrII 3232 CGGTCCG CG^G[AT]CCG
SapI 2229 GAAGAGC GCTCTTC(1/4)
ScaI 1391 AGTACT AGT^ACT
SpeI 4691 ACTAGT A^CTAGT
SwaI 4504 ATTTAAAT ATTT^AAAT
Tth111I 1492 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 897 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 1045 CGCG CG^CG
3171 CGCG
AclI 4946 AACGTT AA^CGTT
6302 AACGTT
AhdI 1865 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2005 GACAAATTGTC
Alw44I 5252 GTGCAC G^TGCAC
5627 GTGCAC
ApaI 2599 GGGCCC GGGCC^C
3583 GGGCCC
ApaLI 5252 GTGCAC G^TGCAC
5627 GTGCAC
Asp700I 1 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3884 GAATACCTTC
AspEI 1865 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2005 GACAAATTGTC
BclI 4110 TGATCA T^GATCA
6149 TGATCA
BglI 1010 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3189 GCCGTCAGGGC
BglII 12 AGATCT A^GATCT
1289 AGATCT
BlpI 624 GCTCAGC GC^T[ACGT]AGC
1540 GCTAAGC
Bpu1102I 624 GCTCAGC GC^T[ACGT]AGC
1540 GCTAAGC
Bsp1407I 6090 TGTACA T^GTACA
6288 TGTACA
BspDI 1773 ATCGAT AT^CGAT
5732 ATCGAT
BsrGI 6090 TGTACA T^GTACA
6288 TGTACA
BstAPI 1343 GCAACGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3180 GCAGGATTTGC
BstUI 1045 CGCG CG^CG
3171 CGCG
BstXI 2745 CCAGGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4590 CCACAGTATTGG
CelII 624 GCTCAGC GC^T[ACGT]AGC
1540 GCTAAGC
ClaI 1773 ATCGAT AT^CGAT
5732 ATCGAT
DraIII 848 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
907 CACAAAGTG
DsaI 293 CCACGG C^C[GA][CT]GG
1069 CCATGG
Eam1105I 1865 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2005 GACAAATTGTC
EcoNI 282 CCTTGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1423 CCTCGACCAGG
EcoT22I 4041 ATGCAT ATGCA^T
4500 ATGCAT
FbaI 4110 TGATCA T^GATCA
6149 TGATCA
HindIII 658 AAGCTT A^AGCTT
5760 AAGCTT
MvnI 1045 CGCG CG^CG
3171 CGCG
NheI 2248 GCTAGC G^CTAGC
3198 GCTAGC
NsiI 4041 ATGCAT ATGCA^T
4500 ATGCAT
PshAI 694 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
878 GACGGAAGTC
Psp1406I 4946 AACGTT AA^CGTT
6302 AACGTT
SacI 5070 GAGCTC GAGCT^C
5828 GAGCTC
SexAI 1219 ACCAGGT A^CC[AT]GGT
2564 ACCTGGT
SmaI 1399 CCCGGG CCC^GGG
2410 CCCGGG
SspBI 6090 TGTACA T^GTACA
6288 TGTACA
SspI 1940 AATATT AAT^ATT
2627 AATATT
XmaCI 1399 CCCGGG C^CCGGG
2410 CCCGGG
XmaI 1399 CCCGGG C^CCGGG
2410 CCCGGG
XmnI 1 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3884 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI Aor51HI ApoI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII