################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AccIII AclI ApaI AscI AspI BcgI BglII BsaBI BseAI BsiWI BspDI BspEI BspHI BssSI BstAPI BstBI BstEII BstPI ClaI CpoI DraIII EcoO65I EcoRI EcoRV FseI HindIII KspI MamI MluI MroI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmeI Psp1406I PvuI RcaI RsrII SacII SapI SfiI SfuI SnaBI SspI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1273 GACGTC GACGT^C AflII 1563 CTTAAG C^TTAAG AgeI 808 ACCGGT A^CCGGT Alw44I 103 GTGCAC G^TGCAC Aor51HI 213 AGCGCT AGC^GCT ApaLI 103 GTGCAC G^TGCAC Asp700I 1955 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 991 TGCGCA TGC^GCA AvrII 231 CCTAGG C^CTAGG BamHI 5367 GGATCC G^GATCC BciVI 1326 GTATCC GTATCC(6/5) BfrI 1563 CTTAAG C^TTAAG BglI 580 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 231 CCTAGG C^CTAGG BsaWI 808 ACCGGT [AT]^CCGG[AT] BsmI 866 GAATGC GAATGC(1/-1) BssHII 432 GCGCGC G^CGCGC Bst1107I 1590 GTATAC GTA^TAC BstZ17I 1590 GTATAC GTA^TAC DraI 4795 TTTAAA TTT^AAA Eco47III 213 AGCGCT AGC^GCT EcoT22I 4457 ATGCAT ATGCA^T FspI 991 TGCGCA TGC^GCA MfeI 74 CAATTG C^AATTG MunI 74 CAATTG C^AATTG NcoI 2627 CCATGG C^CATGG NsiI 4457 ATGCAT ATGCA^T PflMI 4411 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 808 ACCGGT A^CCGGT PshAI 4049 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2323 GAGCTC GAGCT^C SalI 2758 GTCGAC G^TCGAC SbfI 3263 CCTGCAGG CCTGCA^GG ScaI 3020 AGTACT AGT^ACT SexAI 4787 ACCAGGT A^CC[AT]GGT SmaI 4020 CCCGGG CCC^GGG SpeI 3008 ACTAGT A^CTAGT Sse8387I 3263 CCTGCAGG CCTGCA^GG SwaI 4794 ATTTAAAT ATTT^AAAT Van91I 4411 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 4020 CCCGGG C^CCGGG XmaI 4020 CCCGGG C^CCGGG XmnI 1955 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 4557 GGTACC G^GTACC 4784 GGTACC AhdI 348 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5088 GACCAACTGTC AocI 2031 CCTCAGG CC^T[ACGT]AGG 3597 CCTGAGG AseI 4812 ATTAAT AT^TAAT 5022 ATTAAT AsnI 4812 ATTAAT AT^TAAT 5022 ATTAAT Asp718I 4557 GGTACC G^GTACC 4784 GGTACC AspEI 348 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5088 GACCAACTGTC BsiEI 195 CGGCCG CG[GA][CT]^CG 418 CGGCCG BspLU11I 1345 ACATGT A^CATGT 3056 ACATGT BsrBI 395 GAGCGG CCGCTC(-3/-3) 3348 GAGCGG Bsu36I 2031 CCTCAGG CC^T[ACGT]AGG 3597 CCTGAGG DrdI 3116 GACGTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5321 GACCCTGGGGTC EagI 195 CGGCCG C^GGCCG 418 CGGCCG Eam1105I 348 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5088 GACCAACTGTC EclXI 195 CGGCCG C^GGCCG 418 CGGCCG Eco52I 195 CGGCCG C^GGCCG 418 CGGCCG Eco81I 2031 CCTCAGG CC^T[ACGT]AGG 3597 CCTGAGG HpaI 3444 GTTAAC GTT^AAC 4478 GTTAAC KpnI 4557 GGTACC GGTAC^C 4784 GGTACC NaeI 516 GCCGGC GCC^GGC 585 GCCGGC NgoMIV 516 GCCGGC G^CCGGC 585 GCCGGC PshBI 4812 ATTAAT AT^TAAT 5022 ATTAAT SgrAI 515 CGCCGGCG C[GA]^CCGG[CT]G 584 CGCCGGCG SphI 1402 GCATGC GCATG^C 3107 GCATGC StuI 1559 AGGCCT AGG^CCT 2637 AGGCCT XcmI 1465 CCATCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4424 CCAAGAGTCCATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII