##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AccIII      AclI        ApaI        AscI        AspI        
BcgI        BglII       BsaBI       BseAI       BsiWI       
BspDI       BspEI       BspHI       BssSI       BstAPI      
BstBI       BstEII      BstPI       ClaI        CpoI        
DraIII      EcoO65I     EcoRI       EcoRV       FseI        
HindIII     KspI        MamI        MluI        MroI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PmeI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SapI        
SfiI        SfuI        SnaBI       SspI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1273  GACGTC               GACGT^C
AflII        1563  CTTAAG               C^TTAAG
AgeI          808  ACCGGT               A^CCGGT
Alw44I        103  GTGCAC               G^TGCAC
Aor51HI       213  AGCGCT               AGC^GCT
ApaLI         103  GTGCAC               G^TGCAC
Asp700I      1955  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         991  TGCGCA               TGC^GCA
AvrII         231  CCTAGG               C^CTAGG
BamHI        5367  GGATCC               G^GATCC
BciVI        1326  GTATCC               GTATCC(6/5)
BfrI         1563  CTTAAG               C^TTAAG
BglI          580  GCCGCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          231  CCTAGG               C^CTAGG
BsaWI         808  ACCGGT               [AT]^CCGG[AT]
BsmI          866  GAATGC               GAATGC(1/-1)
BssHII        432  GCGCGC               G^CGCGC
Bst1107I     1590  GTATAC               GTA^TAC
BstZ17I      1590  GTATAC               GTA^TAC
DraI         4795  TTTAAA               TTT^AAA
Eco47III      213  AGCGCT               AGC^GCT
EcoT22I      4457  ATGCAT               ATGCA^T
FspI          991  TGCGCA               TGC^GCA
MfeI           74  CAATTG               C^AATTG
MunI           74  CAATTG               C^AATTG
NcoI         2627  CCATGG               C^CATGG
NsiI         4457  ATGCAT               ATGCA^T
PflMI        4411  CCATTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         808  ACCGGT               A^CCGGT
PshAI        4049  GACTTTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2323  GAGCTC               GAGCT^C
SalI         2758  GTCGAC               G^TCGAC
SbfI         3263  CCTGCAGG             CCTGCA^GG
ScaI         3020  AGTACT               AGT^ACT
SexAI        4787  ACCAGGT              A^CC[AT]GGT
SmaI         4020  CCCGGG               CCC^GGG
SpeI         3008  ACTAGT               A^CTAGT
Sse8387I     3263  CCTGCAGG             CCTGCA^GG
SwaI         4794  ATTTAAAT             ATTT^AAAT
Van91I       4411  CCATTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        4020  CCCGGG               C^CCGGG
XmaI         4020  CCCGGG               C^CCGGG
XmnI         1955  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       4557  GGTACC               G^GTACC
             4784  GGTACC              
AhdI          348  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5088  GACCAACTGTC         
AocI         2031  CCTCAGG              CC^T[ACGT]AGG
             3597  CCTGAGG             
AseI         4812  ATTAAT               AT^TAAT
             5022  ATTAAT              
AsnI         4812  ATTAAT               AT^TAAT
             5022  ATTAAT              
Asp718I      4557  GGTACC               G^GTACC
             4784  GGTACC              
AspEI         348  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5088  GACCAACTGTC         
BsiEI         195  CGGCCG               CG[GA][CT]^CG
              418  CGGCCG              
BspLU11I     1345  ACATGT               A^CATGT
             3056  ACATGT              
BsrBI         395  GAGCGG               CCGCTC(-3/-3)
             3348  GAGCGG              
Bsu36I       2031  CCTCAGG              CC^T[ACGT]AGG
             3597  CCTGAGG             
DrdI         3116  GACGTTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5321  GACCCTGGGGTC        
EagI          195  CGGCCG               C^GGCCG
              418  CGGCCG              
Eam1105I      348  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5088  GACCAACTGTC         
EclXI         195  CGGCCG               C^GGCCG
              418  CGGCCG              
Eco52I        195  CGGCCG               C^GGCCG
              418  CGGCCG              
Eco81I       2031  CCTCAGG              CC^T[ACGT]AGG
             3597  CCTGAGG             
HpaI         3444  GTTAAC               GTT^AAC
             4478  GTTAAC              
KpnI         4557  GGTACC               GGTAC^C
             4784  GGTACC              
NaeI          516  GCCGGC               GCC^GGC
              585  GCCGGC              
NgoMIV        516  GCCGGC               G^CCGGC
              585  GCCGGC              
PshBI        4812  ATTAAT               AT^TAAT
             5022  ATTAAT              
SgrAI         515  CGCCGGCG             C[GA]^CCGG[CT]G
              584  CGCCGGCG            
SphI         1402  GCATGC               GCATG^C
             3107  GCATGC              
StuI         1559  AGGCCT               AGG^CCT
             2637  AGGCCT              
XcmI         1465  CCATCATTGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4424  CCAAGAGTCCATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BclI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII