##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AclI ApaI AscI AspI
BcgI BglII BsaBI BseAI BsiWI
BspDI BspEI BspHI BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraIII EcoO65I EcoRI EcoRV FseI
HindIII KspI MamI MluI MroI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI Psp1406I
PvuI RcaI RsrII SacII SapI
SfiI SfuI SnaBI SspI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1273 GACGTC GACGT^C
AflII 1563 CTTAAG C^TTAAG
AgeI 808 ACCGGT A^CCGGT
Alw44I 103 GTGCAC G^TGCAC
Aor51HI 213 AGCGCT AGC^GCT
ApaLI 103 GTGCAC G^TGCAC
Asp700I 1955 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 991 TGCGCA TGC^GCA
AvrII 231 CCTAGG C^CTAGG
BamHI 5367 GGATCC G^GATCC
BciVI 1326 GTATCC GTATCC(6/5)
BfrI 1563 CTTAAG C^TTAAG
BglI 580 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 231 CCTAGG C^CTAGG
BsaWI 808 ACCGGT [AT]^CCGG[AT]
BsmI 866 GAATGC GAATGC(1/-1)
BssHII 432 GCGCGC G^CGCGC
Bst1107I 1590 GTATAC GTA^TAC
BstZ17I 1590 GTATAC GTA^TAC
DraI 4795 TTTAAA TTT^AAA
Eco47III 213 AGCGCT AGC^GCT
EcoT22I 4457 ATGCAT ATGCA^T
FspI 991 TGCGCA TGC^GCA
MfeI 74 CAATTG C^AATTG
MunI 74 CAATTG C^AATTG
NcoI 2627 CCATGG C^CATGG
NsiI 4457 ATGCAT ATGCA^T
PflMI 4411 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 808 ACCGGT A^CCGGT
PshAI 4049 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2323 GAGCTC GAGCT^C
SalI 2758 GTCGAC G^TCGAC
SbfI 3263 CCTGCAGG CCTGCA^GG
ScaI 3020 AGTACT AGT^ACT
SexAI 4787 ACCAGGT A^CC[AT]GGT
SmaI 4020 CCCGGG CCC^GGG
SpeI 3008 ACTAGT A^CTAGT
Sse8387I 3263 CCTGCAGG CCTGCA^GG
SwaI 4794 ATTTAAAT ATTT^AAAT
Van91I 4411 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4020 CCCGGG C^CCGGG
XmaI 4020 CCCGGG C^CCGGG
XmnI 1955 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 4557 GGTACC G^GTACC
4784 GGTACC
AhdI 348 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5088 GACCAACTGTC
AocI 2031 CCTCAGG CC^T[ACGT]AGG
3597 CCTGAGG
AseI 4812 ATTAAT AT^TAAT
5022 ATTAAT
AsnI 4812 ATTAAT AT^TAAT
5022 ATTAAT
Asp718I 4557 GGTACC G^GTACC
4784 GGTACC
AspEI 348 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5088 GACCAACTGTC
BsiEI 195 CGGCCG CG[GA][CT]^CG
418 CGGCCG
BspLU11I 1345 ACATGT A^CATGT
3056 ACATGT
BsrBI 395 GAGCGG CCGCTC(-3/-3)
3348 GAGCGG
Bsu36I 2031 CCTCAGG CC^T[ACGT]AGG
3597 CCTGAGG
DrdI 3116 GACGTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5321 GACCCTGGGGTC
EagI 195 CGGCCG C^GGCCG
418 CGGCCG
Eam1105I 348 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5088 GACCAACTGTC
EclXI 195 CGGCCG C^GGCCG
418 CGGCCG
Eco52I 195 CGGCCG C^GGCCG
418 CGGCCG
Eco81I 2031 CCTCAGG CC^T[ACGT]AGG
3597 CCTGAGG
HpaI 3444 GTTAAC GTT^AAC
4478 GTTAAC
KpnI 4557 GGTACC GGTAC^C
4784 GGTACC
NaeI 516 GCCGGC GCC^GGC
585 GCCGGC
NgoMIV 516 GCCGGC G^CCGGC
585 GCCGGC
PshBI 4812 ATTAAT AT^TAAT
5022 ATTAAT
SgrAI 515 CGCCGGCG C[GA]^CCGG[CT]G
584 CGCCGGCG
SphI 1402 GCATGC GCATG^C
3107 GCATGC
StuI 1559 AGGCCT AGG^CCT
2637 AGGCCT
XcmI 1465 CCATCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4424 CCAAGAGTCCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII