##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AscI AseI AsnI
Asp718I AviII BanI BbeI BsaBI
BseAI BsiWI BspEI BssHII BstBI
CpoI DraIII EagI EclXI Eco52I
EcoRV FauI FseI FspI HpaI
KasI KpnI MamI MluI MroI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PflMI PmeI
PshBI PvuI RsrII SalI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI StuI SwaI Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2706 GACGTC GACGT^C
AccII 5461 CGCG CG^CG
AclI 5960 AACGTT AA^CGTT
AcyI 2706 GACGTC G[GA]^CG[CT]C
AflII 2137 CTTAAG C^TTAAG
Alw44I 5004 GTGCAC G^TGCAC
AocI 2095 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2141 AGCGCT AGC^GCT
ApaI 1123 GGGCCC GGGCC^C
ApaLI 5004 GTGCAC G^TGCAC
Asp700I 1020 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3370 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 5307 GCAGCTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2137 CTTAAG C^TTAAG
BglI 1144 GCCTACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 2706 GACGTC G[GA]^CG[CT]C
BsiEI 1727 CGACCG CG[GA][CT]^CG
BsmBI 5215 CGTCTC CGTCTC(1/5)
BspDI 2528 ATCGAT AT^CGAT
BspMI 3434 ACCTGC ACCTGC(4/8)
BsrBI 2578 CCGCTC CCGCTC(-3/-3)
BsrDI 4234 GCAATG GCAATG(2/0)
BssSI 3247 CACGAG CACGAG(-5/-1)
BstEII 4710 GGTGACC G^GT[ACGT]ACC
BstPI 4710 GGTGACC G^GT[ACGT]ACC
BstUI 5461 CGCG CG^CG
Bsu36I 2095 CCTGAGG CC^T[ACGT]AGG
ClaI 2528 ATCGAT AT^CGAT
DraI 2757 TTTAAA TTT^AAA
Eco47III 2141 AGCGCT AGC^GCT
Eco81I 2095 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4710 GGTGACC G^GT[ACGT]ACC
HaeII 2141 AGCGCT [GA]GCGC^[CT]
Hin1I 2706 GACGTC G[GA]^CG[CT]C
KspI 5460 CCGCGG CCGC^GG
MvnI 5461 CGCG CG^CG
NcoI 1221 CCATGG C^CATGG
NheI 5633 GCTAGC G^CTAGC
PaeR7I 5096 CTCGAG C^TCGAG
PflFI 3370 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 5960 AACGTT AA^CGTT
SacII 5460 CCGCGG CCGC^GG
SbfI 2451 CCTGCAGG CCTGCA^GG
SnaBI 4897 TACGTA TAC^GTA
Sse8387I 2451 CCTGCAGG CCTGCA^GG
Tth111I 3370 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 5009 CCACAGAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5096 CTCGAG C^TCGAG
XmnI 1020 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 967 GTATAC GT^[AC][GT]AC
988 GTATAC
AgeI 364 ACCGGT A^CCGGT
4430 ACCGGT
AhdI 3204 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3433 GACCTGCTGTC
AlwNI 5334 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
5652 CAGCCTCTG
AspEI 3204 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3433 GACCTGCTGTC
AvrII 2542 CCTAGG C^CTAGG
3102 CCTAGG
BamHI 3184 GGATCC G^GATCC
4910 GGATCC
BbvCI 2676 CCTCAGC CCTCAGC(-5/-2)
3554 CCTCAGC
BcnI 2163 CCGGG CC^[GC]GG
4044 CCCGG
BlnI 2542 CCTAGG C^CTAGG
3102 CCTAGG
BlpI 2509 GCTAAGC GC^T[ACGT]AGC
4846 GCTGAGC
Bpu1102I 2509 GCTAAGC GC^T[ACGT]AGC
4846 GCTGAGC
Bsp1407I 2570 TGTACA T^GTACA
5809 TGTACA
BsrGI 2570 TGTACA T^GTACA
5809 TGTACA
Bst1107I 967 GTATAC GTA^TAC
988 GTATAC
BstAPI 4141 GCACTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5520 GCATCCGATGC
BstZ17I 967 GTATAC GTA^TAC
988 GTATAC
CelII 2509 GCTAAGC GC^T[ACGT]AGC
4846 GCTGAGC
DrdI 1783 GACCATTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2700 GACTCAGACGTC
DsaI 1221 CCATGG C^C[GA][CT]GG
5460 CCGCGG
Eam1105I 3204 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3433 GACCTGCTGTC
HgaI 2821 GCGTC GACGC(5/10)
5214 GCGTC
HindIII 67 AAGCTT A^AGCTT
3704 AAGCTT
MfeI 3764 CAATTG C^AATTG
4961 CAATTG
MunI 3764 CAATTG C^AATTG
4961 CAATTG
NciI 2163 CCGGG CC^[GC]GG
4044 CCCGG
PinAI 364 ACCGGT A^CCGGT
4430 ACCGGT
PshAI 3076 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4713 GACCTATGTC
SacI 155 GAGCTC GAGCT^C
2003 GAGCTC
ScaI 2378 AGTACT AGT^ACT
5368 AGTACT
SspBI 2570 TGTACA T^GTACA
5809 TGTACA
XbaI 1917 TCTAGA T^CTAGA
2665 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanII BbrPI BbsI BbvI
BciVI BclI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspLU11I BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII