##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccIII AciI AclI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BglI
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HinfI HpaI KasI KspI MaeII
MamI MfeI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI TfiI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1668 GGTACC G^GTACC
AccII 973 CGCG CG^CG
AcyI 1096 GGCGTC G[GA]^CG[CT]C
AflII 914 CTTAAG C^TTAAG
AflIII 972 ACGCGT A^C[GA][CT]GT
Asp718I 1668 GGTACC G^GTACC
AspHI 1178 GTGCTC G[AT]GC[AT]^C
AvaI 740 CTCGGG C^[CT]CG[GA]G
BanI 1668 GGTACC G^G[CT][GA]CC
BcnI 190 CCGGG CC^[GC]GG
BfrI 914 CTTAAG C^TTAAG
BglII 1102 AGATCT A^GATCT
BlpI 1190 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1190 GCTGAGC GC^T[ACGT]AGC
BsaHI 1096 GGCGTC G[GA]^CG[CT]C
BsaI 297 GAGACC GGTCTC(1/5)
BsiHKAI 1178 GTGCTC G[AT]GC[AT]^C
BsoBI 740 CTCGGG C^[CT]CG[GA]G
BstUI 973 CGCG CG^CG
CelII 1190 GCTGAGC GC^T[ACGT]AGC
DraIII 1128 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 410 GACACCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 693 CCGTGG C^C[GA][CT]GG
EcoNI 367 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1354 ATGCAT ATGCA^T
Hin1I 1096 GGCGTC G[GA]^CG[CT]C
HindIII 1529 AAGCTT A^AGCTT
KpnI 1668 GGTACC GGTAC^C
MluI 972 ACGCGT A^CGCGT
MslI 1338 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 973 CGCG CG^CG
MwoI 51 GCTCAGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 190 CCGGG CC^[GC]GG
NsiI 1354 ATGCAT ATGCA^T
SexAI 979 ACCAGGT A^CC[AT]GGT
SphI 681 GCATGC GCATG^C
SspI 880 AATATT AAT^ATT
XcmI 1130 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AvaII 736 GGTCC G^G[AT]CC
1504 GGTCC
BanII 41 GGGCTC G[GA]GC[CT]^C
326 GAGCCC
BcgI 571 GCAGAGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
681 GCATGCCCTTCG
BspHI 483 TCATGA T^CATGA
1337 TCATGA
BsrDI 385 CATTGC GCAATG(2/0)
523 CATTGC
BstF5I 403 CATCC GGATG(2/0)
1389 CATCC
CfoI 31 GCGC GCG^C
782 GCGC
DraI 1036 TTTAAA TTT^AAA
1692 TTTAAA
EcoRI 563 GAATTC G^AATTC
608 GAATTC
EcoT14I 528 CCAAGG C^C[AT][AT]GG
1161 CCTTGG
FokI 403 CATCC GGATG(9/13)
1389 CATCC
HapII 190 CCGG C^CGG
670 CCGG
HgaI 1097 GCGTC GACGC(5/10)
1268 GCGTC
HhaI 31 GCGC GCG^C
782 GCGC
HinP1I 31 GCGC G^CGC
782 GCGC
HincII 130 GTCAAC GT[CT]^[GA]AC
140 GTCAAC
HindII 130 GTCAAC GT[CT]^[GA]AC
140 GTCAAC
HpaII 190 CCGG C^CGG
670 CCGG
MspA1I 181 CAGCTG C[AC]G^C[GT]G
899 CAGCTG
MspI 190 CCGG C^CGG
670 CCGG
NspI 235 GCATGT [GA]CATG^[CT]
681 GCATGC
PflMI 287 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1127 CCACCATGTGG
PpuMI 735 GGGTCCT [GA]G^G[AT]CC[CT]
1503 AGGTCCT
PstI 46 CTGCAG CTGCA^G
105 CTGCAG
PvuII 181 CAGCTG CAG^CTG
899 CAGCTG
RcaI 483 TCATGA T^CATGA
1337 TCATGA
SmlI 476 CTTGAG C^T[CT][GA]AG
914 CTTAAG
StuI 174 AGGCCT AGG^CCT
1564 AGGCCT
StyI 528 CCAAGG C^C[AT][AT]GG
1161 CCTTGG
TaqI 279 TCGA T^CGA
768 TCGA
TthHB8I 279 TCGA T^CGA
768 TCGA
Van91I 287 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1127 CCACCATGTGG
XbaI 875 TCTAGA T^CTAGA
1660 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
HaeIII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII