################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccI AccIII AciI AclI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BglI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HinfI HpaI KasI KspI MaeII MamI MfeI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI TfiI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 1668 GGTACC G^GTACC AccII 973 CGCG CG^CG AcyI 1096 GGCGTC G[GA]^CG[CT]C AflII 914 CTTAAG C^TTAAG AflIII 972 ACGCGT A^C[GA][CT]GT Asp718I 1668 GGTACC G^GTACC AspHI 1178 GTGCTC G[AT]GC[AT]^C AvaI 740 CTCGGG C^[CT]CG[GA]G BanI 1668 GGTACC G^G[CT][GA]CC BcnI 190 CCGGG CC^[GC]GG BfrI 914 CTTAAG C^TTAAG BglII 1102 AGATCT A^GATCT BlpI 1190 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1190 GCTGAGC GC^T[ACGT]AGC BsaHI 1096 GGCGTC G[GA]^CG[CT]C BsaI 297 GAGACC GGTCTC(1/5) BsiHKAI 1178 GTGCTC G[AT]GC[AT]^C BsoBI 740 CTCGGG C^[CT]CG[GA]G BstUI 973 CGCG CG^CG CelII 1190 GCTGAGC GC^T[ACGT]AGC DraIII 1128 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 410 GACACCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 693 CCGTGG C^C[GA][CT]GG EcoNI 367 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1354 ATGCAT ATGCA^T Hin1I 1096 GGCGTC G[GA]^CG[CT]C HindIII 1529 AAGCTT A^AGCTT KpnI 1668 GGTACC GGTAC^C MluI 972 ACGCGT A^CGCGT MslI 1338 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 973 CGCG CG^CG MwoI 51 GCTCAGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 190 CCGGG CC^[GC]GG NsiI 1354 ATGCAT ATGCA^T SexAI 979 ACCAGGT A^CC[AT]GGT SphI 681 GCATGC GCATG^C SspI 880 AATATT AAT^ATT XcmI 1130 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AvaII 736 GGTCC G^G[AT]CC 1504 GGTCC BanII 41 GGGCTC G[GA]GC[CT]^C 326 GAGCCC BcgI 571 GCAGAGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 681 GCATGCCCTTCG BspHI 483 TCATGA T^CATGA 1337 TCATGA BsrDI 385 CATTGC GCAATG(2/0) 523 CATTGC BstF5I 403 CATCC GGATG(2/0) 1389 CATCC CfoI 31 GCGC GCG^C 782 GCGC DraI 1036 TTTAAA TTT^AAA 1692 TTTAAA EcoRI 563 GAATTC G^AATTC 608 GAATTC EcoT14I 528 CCAAGG C^C[AT][AT]GG 1161 CCTTGG FokI 403 CATCC GGATG(9/13) 1389 CATCC HapII 190 CCGG C^CGG 670 CCGG HgaI 1097 GCGTC GACGC(5/10) 1268 GCGTC HhaI 31 GCGC GCG^C 782 GCGC HinP1I 31 GCGC G^CGC 782 GCGC HincII 130 GTCAAC GT[CT]^[GA]AC 140 GTCAAC HindII 130 GTCAAC GT[CT]^[GA]AC 140 GTCAAC HpaII 190 CCGG C^CGG 670 CCGG MspA1I 181 CAGCTG C[AC]G^C[GT]G 899 CAGCTG MspI 190 CCGG C^CGG 670 CCGG NspI 235 GCATGT [GA]CATG^[CT] 681 GCATGC PflMI 287 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1127 CCACCATGTGG PpuMI 735 GGGTCCT [GA]G^G[AT]CC[CT] 1503 AGGTCCT PstI 46 CTGCAG CTGCA^G 105 CTGCAG PvuII 181 CAGCTG CAG^CTG 899 CAGCTG RcaI 483 TCATGA T^CATGA 1337 TCATGA SmlI 476 CTTGAG C^T[CT][GA]AG 914 CTTAAG StuI 174 AGGCCT AGG^CCT 1564 AGGCCT StyI 528 CCAAGG C^C[AT][AT]GG 1161 CCTTGG TaqI 279 TCGA T^CGA 768 TCGA TthHB8I 279 TCGA T^CGA 768 TCGA Van91I 287 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1127 CCACCATGTGG XbaI 875 TCTAGA T^CTAGA 1660 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XhoII