##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AccI        AccIII      AciI        AclI        
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BglI        
BlnI        BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HinfI       HpaI        KasI        KspI        MaeII       
MamI        MfeI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SwaI        TfiI        
Tth111I     XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       1668  GGTACC               G^GTACC
AccII         973  CGCG                 CG^CG
AcyI         1096  GGCGTC               G[GA]^CG[CT]C
AflII         914  CTTAAG               C^TTAAG
AflIII        972  ACGCGT               A^C[GA][CT]GT
Asp718I      1668  GGTACC               G^GTACC
AspHI        1178  GTGCTC               G[AT]GC[AT]^C
AvaI          740  CTCGGG               C^[CT]CG[GA]G
BanI         1668  GGTACC               G^G[CT][GA]CC
BcnI          190  CCGGG                CC^[GC]GG
BfrI          914  CTTAAG               C^TTAAG
BglII        1102  AGATCT               A^GATCT
BlpI         1190  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1190  GCTGAGC              GC^T[ACGT]AGC
BsaHI        1096  GGCGTC               G[GA]^CG[CT]C
BsaI          297  GAGACC               GGTCTC(1/5)
BsiHKAI      1178  GTGCTC               G[AT]GC[AT]^C
BsoBI         740  CTCGGG               C^[CT]CG[GA]G
BstUI         973  CGCG                 CG^CG
CelII        1190  GCTGAGC              GC^T[ACGT]AGC
DraIII       1128  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          410  GACACCCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          693  CCGTGG               C^C[GA][CT]GG
EcoNI         367  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1354  ATGCAT               ATGCA^T
Hin1I        1096  GGCGTC               G[GA]^CG[CT]C
HindIII      1529  AAGCTT               A^AGCTT
KpnI         1668  GGTACC               GGTAC^C
MluI          972  ACGCGT               A^CGCGT
MslI         1338  CATGAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          973  CGCG                 CG^CG
MwoI           51  GCTCAGGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          190  CCGGG                CC^[GC]GG
NsiI         1354  ATGCAT               ATGCA^T
SexAI         979  ACCAGGT              A^CC[AT]GGT
SphI          681  GCATGC               GCATG^C
SspI          880  AATATT               AAT^ATT
XcmI         1130  CCATGTGGTTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AvaII         736  GGTCC                G^G[AT]CC
             1504  GGTCC               
BanII          41  GGGCTC               G[GA]GC[CT]^C
              326  GAGCCC              
BcgI          571  GCAGAGGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              681  GCATGCCCTTCG        
BspHI         483  TCATGA               T^CATGA
             1337  TCATGA              
BsrDI         385  CATTGC               GCAATG(2/0)
              523  CATTGC              
BstF5I        403  CATCC                GGATG(2/0)
             1389  CATCC               
CfoI           31  GCGC                 GCG^C
              782  GCGC                
DraI         1036  TTTAAA               TTT^AAA
             1692  TTTAAA              
EcoRI         563  GAATTC               G^AATTC
              608  GAATTC              
EcoT14I       528  CCAAGG               C^C[AT][AT]GG
             1161  CCTTGG              
FokI          403  CATCC                GGATG(9/13)
             1389  CATCC               
HapII         190  CCGG                 C^CGG
              670  CCGG                
HgaI         1097  GCGTC                GACGC(5/10)
             1268  GCGTC               
HhaI           31  GCGC                 GCG^C
              782  GCGC                
HinP1I         31  GCGC                 G^CGC
              782  GCGC                
HincII        130  GTCAAC               GT[CT]^[GA]AC
              140  GTCAAC              
HindII        130  GTCAAC               GT[CT]^[GA]AC
              140  GTCAAC              
HpaII         190  CCGG                 C^CGG
              670  CCGG                
MspA1I        181  CAGCTG               C[AC]G^C[GT]G
              899  CAGCTG              
MspI          190  CCGG                 C^CGG
              670  CCGG                
NspI          235  GCATGT               [GA]CATG^[CT]
              681  GCATGC              
PflMI         287  CCAGGCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1127  CCACCATGTGG         
PpuMI         735  GGGTCCT              [GA]G^G[AT]CC[CT]
             1503  AGGTCCT             
PstI           46  CTGCAG               CTGCA^G
              105  CTGCAG              
PvuII         181  CAGCTG               CAG^CTG
              899  CAGCTG              
RcaI          483  TCATGA               T^CATGA
             1337  TCATGA              
SmlI          476  CTTGAG               C^T[CT][GA]AG
              914  CTTAAG              
StuI          174  AGGCCT               AGG^CCT
             1564  AGGCCT              
StyI          528  CCAAGG               C^C[AT][AT]GG
             1161  CCTTGG              
TaqI          279  TCGA                 T^CGA
              768  TCGA                
TthHB8I       279  TCGA                 T^CGA
              768  TCGA                
Van91I        287  CCAGGCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1127  CCACCATGTGG         
XbaI          875  TCTAGA               T^CTAGA
             1660  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       BstXI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
HaeIII      HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MvaI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII