##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AflIII AgeI ApaI
AscI AseI AsnI AspI BalI
BamHI BbeI BbrPI BcgI BsaAI
BsaBI BsiEI BsiWI BspLU11I BsrFI
Bst1107I BstAPI BstBI BstZ17I Cfr10I
CpoI EaeI EagI EclXI Eco52I
FseI HincII HindII HpaI KasI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 5053 GTAGAC GT^[AC][GT]AC
AccIII 1316 TCCGGA T^CCGGA
AflII 2443 CTTAAG C^TTAAG
Alw44I 4016 GTGCAC G^TGCAC
ApaLI 4016 GTGCAC G^TGCAC
Asp700I 5023 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3848 TGCGCA TGC^GCA
BclI 1043 TGATCA T^GATCA
BfrI 2443 CTTAAG C^TTAAG
BseAI 1316 TCCGGA T^CCGGA
BsmBI 4890 CGTCTC CGTCTC(1/5)
BspDI 2375 ATCGAT AT^CGAT
BspEI 1316 TCCGGA T^CCGGA
BspHI 1919 TCATGA T^CATGA
BsrBI 4355 CCGCTC CCGCTC(-3/-3)
BssHII 1584 GCGCGC G^CGCGC
BssSI 4011 CACGAG CACGAG(-5/-1)
BstEII 5116 GGTGACC G^GT[ACGT]ACC
BstPI 5116 GGTGACC G^GT[ACGT]ACC
ClaI 2375 ATCGAT AT^CGAT
EcoO65I 5116 GGTGACC G^GT[ACGT]ACC
EcoRV 4976 GATATC GAT^ATC
FbaI 1043 TGATCA T^GATCA
FspI 3848 TGCGCA TGC^GCA
HindIII 5408 AAGCTT A^AGCTT
MroI 1316 TCCGGA T^CCGGA
PshAI 5119 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3475 CAGCTG CAG^CTG
RcaI 1919 TCATGA T^CATGA
SapI 4460 GAAGAGC GCTCTTC(1/4)
SexAI 3415 ACCTGGT A^CC[AT]GGT
XcmI 3399 CCACACCTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 5023 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3412 GGTACC G^GTACC
4948 GGTACC
AccII 1585 CGCG CG^CG
3993 CGCG
AcyI 58 GACGCC G[GA]^CG[CT]C
258 GACGCC
Aor51HI 3796 AGCGCT AGC^GCT
4627 AGCGCT
Asp718I 3412 GGTACC G^GTACC
4948 GGTACC
AvaI 1458 CCCGAG C^[CT]CG[GA]G
5388 CTCGGG
BbvCI 351 CCTCAGC CCTCAGC(-5/-2)
3980 GCTGAGG
BglI 3443 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5386 GCCTCGGGGGC
BglII 1904 AGATCT A^GATCT
3492 AGATCT
BsaHI 58 GACGCC G[GA]^CG[CT]C
258 GACGCC
BsaWI 1316 TCCGGA [AT]^CCGG[AT]
5120 ACCGGA
BsmI 209 GAATGC GAATGC(1/-1)
3035 GCATTC
BsoBI 1458 CCCGAG C^[CT]CG[GA]G
5388 CTCGGG
Bsp1407I 662 TGTACA T^GTACA
1359 TGTACA
BsrDI 474 CATTGC GCAATG(2/0)
913 GCAATG
BsrGI 662 TGTACA T^GTACA
1359 TGTACA
BstUI 1585 CGCG CG^CG
3993 CGCG
DraIII 2007 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4344 CACAGGGTG
DrdI 157 GACTCAACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
258 GACGCCCCTGTC
Eco47III 3796 AGCGCT AGC^GCT
4627 AGCGCT
EcoNI 5028 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5233 CCTAGGGAAGG
EcoT22I 1911 ATGCAT ATGCA^T
3782 ATGCAT
FauI 3837 GCGGG CCCGC(4/6)
4256 GCGGG
Hin1I 58 GACGCC G[GA]^CG[CT]C
258 GACGCC
KpnI 3412 GGTACC GGTAC^C
4948 GGTACC
MaeII 4889 ACGT A^CGT
5057 ACGT
MvnI 1585 CGCG CG^CG
3993 CGCG
NcoI 2663 CCATGG C^CATGG
4116 CCATGG
NsiI 1911 ATGCAT ATGCA^T
3782 ATGCAT
PflMI 73 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5034 CCAGGCCCTGG
PpuMI 68 AGGTCCC [GA]G^G[AT]CC[CT]
4203 AGGACCT
SspBI 662 TGTACA T^GTACA
1359 TGTACA
Van91I 73 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5034 CCAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI AocI ApoI AspEI
AspHI AvaII AvrII BanI BanII
BbsI BbvI BciVI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI Eam1105I EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII