##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       AclI        AflIII      AgeI        ApaI        
AscI        AseI        AsnI        AspI        BalI        
BamHI       BbeI        BbrPI       BcgI        BsaAI       
BsaBI       BsiEI       BsiWI       BspLU11I    BsrFI       
Bst1107I    BstAPI      BstBI       BstZ17I     Cfr10I      
CpoI        EaeI        EagI        EclXI       Eco52I      
FseI        HincII      HindII      HpaI        KasI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI         5053  GTAGAC               GT^[AC][GT]AC
AccIII       1316  TCCGGA               T^CCGGA
AflII        2443  CTTAAG               C^TTAAG
Alw44I       4016  GTGCAC               G^TGCAC
ApaLI        4016  GTGCAC               G^TGCAC
Asp700I      5023  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        3848  TGCGCA               TGC^GCA
BclI         1043  TGATCA               T^GATCA
BfrI         2443  CTTAAG               C^TTAAG
BseAI        1316  TCCGGA               T^CCGGA
BsmBI        4890  CGTCTC               CGTCTC(1/5)
BspDI        2375  ATCGAT               AT^CGAT
BspEI        1316  TCCGGA               T^CCGGA
BspHI        1919  TCATGA               T^CATGA
BsrBI        4355  CCGCTC               CCGCTC(-3/-3)
BssHII       1584  GCGCGC               G^CGCGC
BssSI        4011  CACGAG               CACGAG(-5/-1)
BstEII       5116  GGTGACC              G^GT[ACGT]ACC
BstPI        5116  GGTGACC              G^GT[ACGT]ACC
ClaI         2375  ATCGAT               AT^CGAT
EcoO65I      5116  GGTGACC              G^GT[ACGT]ACC
EcoRV        4976  GATATC               GAT^ATC
FbaI         1043  TGATCA               T^GATCA
FspI         3848  TGCGCA               TGC^GCA
HindIII      5408  AAGCTT               A^AGCTT
MroI         1316  TCCGGA               T^CCGGA
PshAI        5119  GACCGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        3475  CAGCTG               CAG^CTG
RcaI         1919  TCATGA               T^CATGA
SapI         4460  GAAGAGC              GCTCTTC(1/4)
SexAI        3415  ACCTGGT              A^CC[AT]GGT
XcmI         3399  CCACACCTTGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         5023  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       3412  GGTACC               G^GTACC
             4948  GGTACC              
AccII        1585  CGCG                 CG^CG
             3993  CGCG                
AcyI           58  GACGCC               G[GA]^CG[CT]C
              258  GACGCC              
Aor51HI      3796  AGCGCT               AGC^GCT
             4627  AGCGCT              
Asp718I      3412  GGTACC               G^GTACC
             4948  GGTACC              
AvaI         1458  CCCGAG               C^[CT]CG[GA]G
             5388  CTCGGG              
BbvCI         351  CCTCAGC              CCTCAGC(-5/-2)
             3980  GCTGAGG             
BglI         3443  GCCTGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5386  GCCTCGGGGGC         
BglII        1904  AGATCT               A^GATCT
             3492  AGATCT              
BsaHI          58  GACGCC               G[GA]^CG[CT]C
              258  GACGCC              
BsaWI        1316  TCCGGA               [AT]^CCGG[AT]
             5120  ACCGGA              
BsmI          209  GAATGC               GAATGC(1/-1)
             3035  GCATTC              
BsoBI        1458  CCCGAG               C^[CT]CG[GA]G
             5388  CTCGGG              
Bsp1407I      662  TGTACA               T^GTACA
             1359  TGTACA              
BsrDI         474  CATTGC               GCAATG(2/0)
              913  GCAATG              
BsrGI         662  TGTACA               T^GTACA
             1359  TGTACA              
BstUI        1585  CGCG                 CG^CG
             3993  CGCG                
DraIII       2007  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4344  CACAGGGTG           
DrdI          157  GACTCAACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              258  GACGCCCCTGTC        
Eco47III     3796  AGCGCT               AGC^GCT
             4627  AGCGCT              
EcoNI        5028  CCTTCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5233  CCTAGGGAAGG         
EcoT22I      1911  ATGCAT               ATGCA^T
             3782  ATGCAT              
FauI         3837  GCGGG                CCCGC(4/6)
             4256  GCGGG               
Hin1I          58  GACGCC               G[GA]^CG[CT]C
              258  GACGCC              
KpnI         3412  GGTACC               GGTAC^C
             4948  GGTACC              
MaeII        4889  ACGT                 A^CGT
             5057  ACGT                
MvnI         1585  CGCG                 CG^CG
             3993  CGCG                
NcoI         2663  CCATGG               C^CATGG
             4116  CCATGG              
NsiI         1911  ATGCAT               ATGCA^T
             3782  ATGCAT              
PflMI          73  CCACTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5034  CCAGGCCCTGG         
PpuMI          68  AGGTCCC              [GA]G^G[AT]CC[CT]
             4203  AGGACCT             
SspBI         662  TGTACA               T^GTACA
             1359  TGTACA              
Van91I         73  CCACTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5034  CCAGGCCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AciI        AcsI        AfaI        AhdI        AluI        
AlwI        AlwNI       AocI        ApoI        AspEI       
AspHI       AvaII       AvrII       BanI        BanII       
BbsI        BbvI        BciVI       BcnI        BfaI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        Eam1105I    EarI        
Eco81I      EcoO109I    EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        SacI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII