################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AclI AflIII AgeI ApaI AscI AseI AsnI AspI BalI BamHI BbeI BbrPI BcgI BsaAI BsaBI BsiEI BsiWI BspLU11I BsrFI Bst1107I BstAPI BstBI BstZ17I Cfr10I CpoI EaeI EagI EclXI Eco52I FseI HincII HindII HpaI KasI KspI MamI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 5053 GTAGAC GT^[AC][GT]AC AccIII 1316 TCCGGA T^CCGGA AflII 2443 CTTAAG C^TTAAG Alw44I 4016 GTGCAC G^TGCAC ApaLI 4016 GTGCAC G^TGCAC Asp700I 5023 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 3848 TGCGCA TGC^GCA BclI 1043 TGATCA T^GATCA BfrI 2443 CTTAAG C^TTAAG BseAI 1316 TCCGGA T^CCGGA BsmBI 4890 CGTCTC CGTCTC(1/5) BspDI 2375 ATCGAT AT^CGAT BspEI 1316 TCCGGA T^CCGGA BspHI 1919 TCATGA T^CATGA BsrBI 4355 CCGCTC CCGCTC(-3/-3) BssHII 1584 GCGCGC G^CGCGC BssSI 4011 CACGAG CACGAG(-5/-1) BstEII 5116 GGTGACC G^GT[ACGT]ACC BstPI 5116 GGTGACC G^GT[ACGT]ACC ClaI 2375 ATCGAT AT^CGAT EcoO65I 5116 GGTGACC G^GT[ACGT]ACC EcoRV 4976 GATATC GAT^ATC FbaI 1043 TGATCA T^GATCA FspI 3848 TGCGCA TGC^GCA HindIII 5408 AAGCTT A^AGCTT MroI 1316 TCCGGA T^CCGGA PshAI 5119 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 3475 CAGCTG CAG^CTG RcaI 1919 TCATGA T^CATGA SapI 4460 GAAGAGC GCTCTTC(1/4) SexAI 3415 ACCTGGT A^CC[AT]GGT XcmI 3399 CCACACCTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 5023 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 3412 GGTACC G^GTACC 4948 GGTACC AccII 1585 CGCG CG^CG 3993 CGCG AcyI 58 GACGCC G[GA]^CG[CT]C 258 GACGCC Aor51HI 3796 AGCGCT AGC^GCT 4627 AGCGCT Asp718I 3412 GGTACC G^GTACC 4948 GGTACC AvaI 1458 CCCGAG C^[CT]CG[GA]G 5388 CTCGGG BbvCI 351 CCTCAGC CCTCAGC(-5/-2) 3980 GCTGAGG BglI 3443 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5386 GCCTCGGGGGC BglII 1904 AGATCT A^GATCT 3492 AGATCT BsaHI 58 GACGCC G[GA]^CG[CT]C 258 GACGCC BsaWI 1316 TCCGGA [AT]^CCGG[AT] 5120 ACCGGA BsmI 209 GAATGC GAATGC(1/-1) 3035 GCATTC BsoBI 1458 CCCGAG C^[CT]CG[GA]G 5388 CTCGGG Bsp1407I 662 TGTACA T^GTACA 1359 TGTACA BsrDI 474 CATTGC GCAATG(2/0) 913 GCAATG BsrGI 662 TGTACA T^GTACA 1359 TGTACA BstUI 1585 CGCG CG^CG 3993 CGCG DraIII 2007 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 4344 CACAGGGTG DrdI 157 GACTCAACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 258 GACGCCCCTGTC Eco47III 3796 AGCGCT AGC^GCT 4627 AGCGCT EcoNI 5028 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5233 CCTAGGGAAGG EcoT22I 1911 ATGCAT ATGCA^T 3782 ATGCAT FauI 3837 GCGGG CCCGC(4/6) 4256 GCGGG Hin1I 58 GACGCC G[GA]^CG[CT]C 258 GACGCC KpnI 3412 GGTACC GGTAC^C 4948 GGTACC MaeII 4889 ACGT A^CGT 5057 ACGT MvnI 1585 CGCG CG^CG 3993 CGCG NcoI 2663 CCATGG C^CATGG 4116 CCATGG NsiI 1911 ATGCAT ATGCA^T 3782 ATGCAT PflMI 73 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5034 CCAGGCCCTGG PpuMI 68 AGGTCCC [GA]G^G[AT]CC[CT] 4203 AGGACCT SspBI 662 TGTACA T^GTACA 1359 TGTACA Van91I 73 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5034 CCAGGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI AocI ApoI AspEI AspHI AvaII AvrII BanI BanII BbsI BbvI BciVI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI Eam1105I EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII