################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AclI AhdI ApaI AscI Asp718I AspEI AvrII BalI BbeI BbrPI BclI BglII BlnI BsiEI BsiWI Bsp1407I BspDI BspHI BsrGI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I FbaI FseI HpaI KasI KpnI MluI MluNI MscI NaeI NarI NgoMIV NotI NruI NspV PacI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 3020 GACGCC G[GA]^CG[CT]C AgeI 1209 ACCGGT A^CCGGT Alw44I 3512 GTGCAC G^TGCAC AocI 925 CCTGAGG CC^T[ACGT]AGG Aor51HI 4885 AGCGCT AGC^GCT ApaLI 3512 GTGCAC G^TGCAC AseI 2646 ATTAAT AT^TAAT AsnI 2646 ATTAAT AT^TAAT BbvCI 1235 CCTCAGC CCTCAGC(-5/-2) BcnI 1046 CCCGG CC^[GC]GG BsaBI 2954 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3020 GACGCC G[GA]^CG[CT]C BsmBI 446 GAGACG CGTCTC(1/5) BspLU11I 3050 ACATGT A^CATGT BssHII 1924 GCGCGC G^CGCGC BstEII 4866 GGTCACC G^GT[ACGT]ACC BstPI 4866 GGTCACC G^GT[ACGT]ACC Bsu36I 925 CCTGAGG CC^T[ACGT]AGG DraIII 3936 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 545 GACCTAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 5429 TGGCCG [CT]^GGCC[GA] Eco47III 4885 AGCGCT AGC^GCT Eco81I 925 CCTGAGG CC^T[ACGT]AGG EcoO65I 4866 GGTCACC G^GT[ACGT]ACC EcoRI 5583 GAATTC G^AATTC EcoRV 1940 GATATC GAT^ATC Hin1I 3020 GACGCC G[GA]^CG[CT]C KspI 1809 CCGCGG CCGC^GG MamI 2954 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3680 CAATTG C^AATTG MunI 3680 CAATTG C^AATTG NciI 1046 CCCGG CC^[GC]GG NcoI 506 CCATGG C^CATGG NdeI 4587 CATATG CA^TATG PflMI 4138 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1209 ACCGGT A^CCGGT PshAI 5762 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2646 ATTAAT AT^TAAT SacII 1809 CCGCGG CCGC^GG SspI 2042 AATATT AAT^ATT StuI 929 AGGCCT AGG^CCT Van91I 4138 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccIII 1101 TCCGGA T^CCGGA 5635 TCCGGA AcsI 2368 AAATTT [GA]^AATT[CT] 5583 GAATTC AflII 2212 CTTAAG C^TTAAG 2736 CTTAAG AflIII 3050 ACATGT A^C[GA][CT]GT 4647 ACGTGT AlwNI 1060 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 4669 CAGGCGCTG ApoI 2368 AAATTT [GA]^AATT[CT] 5583 GAATTC AspI 4299 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4504 GACCATGTC AviII 2796 TGCGCA TGC^GCA 3499 TGCGCA BamHI 2605 GGATCC G^GATCC 5177 GGATCC BanI 806 GGCACC G^G[CT][GA]CC 2553 GGTGCC BbsI 2348 GAAGAC GAAGAC(2/6) 5769 GTCTTC BcgI 1179 CGACACGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3484 GCATGTGAGTCG BfrI 2212 CTTAAG C^TTAAG 2736 CTTAAG BglI 3851 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4332 GCCTCTAGGGC BpuAI 2348 GAAGAC GAAGAC(2/6) 5769 GTCTTC BseAI 1101 TCCGGA T^CCGGA 5635 TCCGGA BspEI 1101 TCCGGA T^CCGGA 5635 TCCGGA BspMI 3265 ACCTGC ACCTGC(4/8) 1979 GCAGGT BsrBI 891 CCGCTC CCGCTC(-3/-3) 5173 GAGCGG BsrDI 1988 CATTGC GCAATG(2/0) 3014 GCAATG BsrFI 128 GCCGGT [GA]^CCGG[CT] 1209 ACCGGT BssSI 443 CACGAG CACGAG(-5/-1) 1508 CACGAG Cfr10I 128 GCCGGT [GA]^CCGG[CT] 1209 ACCGGT DraII 951 AGGCCCT [GA]G^G[ACGT]CC[CT] 5205 AGGGCCT EcoO109I 951 AGGCCCT [GA]G^G[ACGT]CC[CT] 5205 AGGGCCT EcoT22I 4584 ATGCAT ATGCA^T 5317 ATGCAT FspI 2796 TGCGCA TGC^GCA 3499 TGCGCA HaeII 4671 GGCGCT [GA]GCGC^[CT] 4885 AGCGCT MroI 1101 TCCGGA T^CCGGA 5635 TCCGGA NheI 2206 GCTAGC G^CTAGC 5594 GCTAGC NsiI 4584 ATGCAT ATGCA^T 5317 ATGCAT PaeR7I 563 CTCGAG C^TCGAG 5303 CTCGAG PflFI 4299 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4504 GACCATGTC SpeI 3743 ACTAGT A^CTAGT 5612 ACTAGT Tth111I 4299 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4504 GACCATGTC XcmI 1230 CCAGTCCTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5375 CCAAGAGCCGCCTGG XhoI 563 CTCGAG C^TCGAG 5303 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI Asp700I AspHI AvaI AvaII BanII BbvI BciVI BfaI BlpI BmyI BpmI Bpu1102I BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DsaI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI