##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AhdI ApaI
AscI Asp718I AspEI AvrII BalI
BbeI BbrPI BclI BglII BlnI
BsiEI BsiWI Bsp1407I BspDI BspHI
BsrGI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I FbaI FseI HpaI KasI
KpnI MluI MluNI MscI NaeI
NarI NgoMIV NotI NruI NspV
PacI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 3020 GACGCC G[GA]^CG[CT]C
AgeI 1209 ACCGGT A^CCGGT
Alw44I 3512 GTGCAC G^TGCAC
AocI 925 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4885 AGCGCT AGC^GCT
ApaLI 3512 GTGCAC G^TGCAC
AseI 2646 ATTAAT AT^TAAT
AsnI 2646 ATTAAT AT^TAAT
BbvCI 1235 CCTCAGC CCTCAGC(-5/-2)
BcnI 1046 CCCGG CC^[GC]GG
BsaBI 2954 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3020 GACGCC G[GA]^CG[CT]C
BsmBI 446 GAGACG CGTCTC(1/5)
BspLU11I 3050 ACATGT A^CATGT
BssHII 1924 GCGCGC G^CGCGC
BstEII 4866 GGTCACC G^GT[ACGT]ACC
BstPI 4866 GGTCACC G^GT[ACGT]ACC
Bsu36I 925 CCTGAGG CC^T[ACGT]AGG
DraIII 3936 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 545 GACCTAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 5429 TGGCCG [CT]^GGCC[GA]
Eco47III 4885 AGCGCT AGC^GCT
Eco81I 925 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4866 GGTCACC G^GT[ACGT]ACC
EcoRI 5583 GAATTC G^AATTC
EcoRV 1940 GATATC GAT^ATC
Hin1I 3020 GACGCC G[GA]^CG[CT]C
KspI 1809 CCGCGG CCGC^GG
MamI 2954 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3680 CAATTG C^AATTG
MunI 3680 CAATTG C^AATTG
NciI 1046 CCCGG CC^[GC]GG
NcoI 506 CCATGG C^CATGG
NdeI 4587 CATATG CA^TATG
PflMI 4138 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1209 ACCGGT A^CCGGT
PshAI 5762 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2646 ATTAAT AT^TAAT
SacII 1809 CCGCGG CCGC^GG
SspI 2042 AATATT AAT^ATT
StuI 929 AGGCCT AGG^CCT
Van91I 4138 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 1101 TCCGGA T^CCGGA
5635 TCCGGA
AcsI 2368 AAATTT [GA]^AATT[CT]
5583 GAATTC
AflII 2212 CTTAAG C^TTAAG
2736 CTTAAG
AflIII 3050 ACATGT A^C[GA][CT]GT
4647 ACGTGT
AlwNI 1060 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
4669 CAGGCGCTG
ApoI 2368 AAATTT [GA]^AATT[CT]
5583 GAATTC
AspI 4299 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4504 GACCATGTC
AviII 2796 TGCGCA TGC^GCA
3499 TGCGCA
BamHI 2605 GGATCC G^GATCC
5177 GGATCC
BanI 806 GGCACC G^G[CT][GA]CC
2553 GGTGCC
BbsI 2348 GAAGAC GAAGAC(2/6)
5769 GTCTTC
BcgI 1179 CGACACGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3484 GCATGTGAGTCG
BfrI 2212 CTTAAG C^TTAAG
2736 CTTAAG
BglI 3851 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4332 GCCTCTAGGGC
BpuAI 2348 GAAGAC GAAGAC(2/6)
5769 GTCTTC
BseAI 1101 TCCGGA T^CCGGA
5635 TCCGGA
BspEI 1101 TCCGGA T^CCGGA
5635 TCCGGA
BspMI 3265 ACCTGC ACCTGC(4/8)
1979 GCAGGT
BsrBI 891 CCGCTC CCGCTC(-3/-3)
5173 GAGCGG
BsrDI 1988 CATTGC GCAATG(2/0)
3014 GCAATG
BsrFI 128 GCCGGT [GA]^CCGG[CT]
1209 ACCGGT
BssSI 443 CACGAG CACGAG(-5/-1)
1508 CACGAG
Cfr10I 128 GCCGGT [GA]^CCGG[CT]
1209 ACCGGT
DraII 951 AGGCCCT [GA]G^G[ACGT]CC[CT]
5205 AGGGCCT
EcoO109I 951 AGGCCCT [GA]G^G[ACGT]CC[CT]
5205 AGGGCCT
EcoT22I 4584 ATGCAT ATGCA^T
5317 ATGCAT
FspI 2796 TGCGCA TGC^GCA
3499 TGCGCA
HaeII 4671 GGCGCT [GA]GCGC^[CT]
4885 AGCGCT
MroI 1101 TCCGGA T^CCGGA
5635 TCCGGA
NheI 2206 GCTAGC G^CTAGC
5594 GCTAGC
NsiI 4584 ATGCAT ATGCA^T
5317 ATGCAT
PaeR7I 563 CTCGAG C^TCGAG
5303 CTCGAG
PflFI 4299 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4504 GACCATGTC
SpeI 3743 ACTAGT A^CTAGT
5612 ACTAGT
Tth111I 4299 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4504 GACCATGTC
XcmI 1230 CCAGTCCTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5375 CCAAGAGCCGCCTGG
XhoI 563 CTCGAG C^TCGAG
5303 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI Asp700I AspHI AvaI AvaII
BanII BbvI BciVI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI