##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AclI        AhdI        ApaI        
AscI        Asp718I     AspEI       AvrII       BalI        
BbeI        BbrPI       BclI        BglII       BlnI        
BsiEI       BsiWI       Bsp1407I    BspDI       BspHI       
BsrGI       Bst1107I    BstAPI      BstBI       BstZ17I     
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco52I      FbaI        FseI        HpaI        KasI        
KpnI        MluI        MluNI       MscI        NaeI        
NarI        NgoMIV      NotI        NruI        NspV        
PacI        PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI         3020  GACGCC               G[GA]^CG[CT]C
AgeI         1209  ACCGGT               A^CCGGT
Alw44I       3512  GTGCAC               G^TGCAC
AocI          925  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      4885  AGCGCT               AGC^GCT
ApaLI        3512  GTGCAC               G^TGCAC
AseI         2646  ATTAAT               AT^TAAT
AsnI         2646  ATTAAT               AT^TAAT
BbvCI        1235  CCTCAGC              CCTCAGC(-5/-2)
BcnI         1046  CCCGG                CC^[GC]GG
BsaBI        2954  GATACCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        3020  GACGCC               G[GA]^CG[CT]C
BsmBI         446  GAGACG               CGTCTC(1/5)
BspLU11I     3050  ACATGT               A^CATGT
BssHII       1924  GCGCGC               G^CGCGC
BstEII       4866  GGTCACC              G^GT[ACGT]ACC
BstPI        4866  GGTCACC              G^GT[ACGT]ACC
Bsu36I        925  CCTGAGG              CC^T[ACGT]AGG
DraIII       3936  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          545  GACCTAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         5429  TGGCCG               [CT]^GGCC[GA]
Eco47III     4885  AGCGCT               AGC^GCT
Eco81I        925  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      4866  GGTCACC              G^GT[ACGT]ACC
EcoRI        5583  GAATTC               G^AATTC
EcoRV        1940  GATATC               GAT^ATC
Hin1I        3020  GACGCC               G[GA]^CG[CT]C
KspI         1809  CCGCGG               CCGC^GG
MamI         2954  GATACCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3680  CAATTG               C^AATTG
MunI         3680  CAATTG               C^AATTG
NciI         1046  CCCGG                CC^[GC]GG
NcoI          506  CCATGG               C^CATGG
NdeI         4587  CATATG               CA^TATG
PflMI        4138  CCACTGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1209  ACCGGT               A^CCGGT
PshAI        5762  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2646  ATTAAT               AT^TAAT
SacII        1809  CCGCGG               CCGC^GG
SspI         2042  AATATT               AAT^ATT
StuI          929  AGGCCT               AGG^CCT
Van91I       4138  CCACTGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccIII       1101  TCCGGA               T^CCGGA
             5635  TCCGGA              
AcsI         2368  AAATTT               [GA]^AATT[CT]
             5583  GAATTC              
AflII        2212  CTTAAG               C^TTAAG
             2736  CTTAAG              
AflIII       3050  ACATGT               A^C[GA][CT]GT
             4647  ACGTGT              
AlwNI        1060  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4669  CAGGCGCTG           
ApoI         2368  AAATTT               [GA]^AATT[CT]
             5583  GAATTC              
AspI         4299  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4504  GACCATGTC           
AviII        2796  TGCGCA               TGC^GCA
             3499  TGCGCA              
BamHI        2605  GGATCC               G^GATCC
             5177  GGATCC              
BanI          806  GGCACC               G^G[CT][GA]CC
             2553  GGTGCC              
BbsI         2348  GAAGAC               GAAGAC(2/6)
             5769  GTCTTC              
BcgI         1179  CGACACGGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3484  GCATGTGAGTCG        
BfrI         2212  CTTAAG               C^TTAAG
             2736  CTTAAG              
BglI         3851  GCCGAGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4332  GCCTCTAGGGC         
BpuAI        2348  GAAGAC               GAAGAC(2/6)
             5769  GTCTTC              
BseAI        1101  TCCGGA               T^CCGGA
             5635  TCCGGA              
BspEI        1101  TCCGGA               T^CCGGA
             5635  TCCGGA              
BspMI        3265  ACCTGC               ACCTGC(4/8)
             1979  GCAGGT              
BsrBI         891  CCGCTC               CCGCTC(-3/-3)
             5173  GAGCGG              
BsrDI        1988  CATTGC               GCAATG(2/0)
             3014  GCAATG              
BsrFI         128  GCCGGT               [GA]^CCGG[CT]
             1209  ACCGGT              
BssSI         443  CACGAG               CACGAG(-5/-1)
             1508  CACGAG              
Cfr10I        128  GCCGGT               [GA]^CCGG[CT]
             1209  ACCGGT              
DraII         951  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             5205  AGGGCCT             
EcoO109I      951  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             5205  AGGGCCT             
EcoT22I      4584  ATGCAT               ATGCA^T
             5317  ATGCAT              
FspI         2796  TGCGCA               TGC^GCA
             3499  TGCGCA              
HaeII        4671  GGCGCT               [GA]GCGC^[CT]
             4885  AGCGCT              
MroI         1101  TCCGGA               T^CCGGA
             5635  TCCGGA              
NheI         2206  GCTAGC               G^CTAGC
             5594  GCTAGC              
NsiI         4584  ATGCAT               ATGCA^T
             5317  ATGCAT              
PaeR7I        563  CTCGAG               C^TCGAG
             5303  CTCGAG              
PflFI        4299  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4504  GACCATGTC           
SpeI         3743  ACTAGT               A^CTAGT
             5612  ACTAGT              
Tth111I      4299  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4504  GACCATGTC           
XcmI         1230  CCAGTCCTCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5375  CCAAGAGCCGCCTGG     
XhoI          563  CTCGAG               C^TCGAG
             5303  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AluI        
AlwI        Asp700I     AspHI       AvaI        AvaII       
BanII       BbvI        BciVI       BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraI        DsaI        EarI        EcoNI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII       XmnI