##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BbeI
BbrPI BcgI BcnI BglI BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BspDI BspEI BspLU11I BsrFI BssHII
BssSI Bst1107I BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 804 GACGTC GACGT^C
AccI 1456 GTAGAC GT^[AC][GT]AC
AflII 606 CTTAAG C^TTAAG
AflIII 2331 ACGTGT A^C[GA][CT]GT
Asp700I 1917 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 40 CTCGAG C^[CT]CG[GA]G
BamHI 1800 GGATCC G^GATCC
BbvCI 2101 CCTCAGC CCTCAGC(-5/-2)
BciVI 266 GTATCC GTATCC(6/5)
BclI 678 TGATCA T^GATCA
BfrI 606 CTTAAG C^TTAAG
BglII 648 AGATCT A^GATCT
BsaI 363 GGTCTC GGTCTC(1/5)
BsaWI 464 TCCGGT [AT]^CCGG[AT]
BsiEI 466 CGGTCG CG[GA][CT]^CG
BsmI 1231 GCATTC GAATGC(1/-1)
BsoBI 40 CTCGAG C^[CT]CG[GA]G
Bsp1407I 779 TGTACA T^GTACA
BsrBI 1267 GAGCGG CCGCTC(-3/-3)
BsrDI 439 CATTGC GCAATG(2/0)
BsrGI 779 TGTACA T^GTACA
BstAPI 302 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2165 TTCGAA TT^CGAA
BstEII 1909 GGTGACC G^GT[ACGT]ACC
BstPI 1909 GGTGACC G^GT[ACGT]ACC
DraII 1866 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2229 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2258 GACTTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1118 CCATGG C^C[GA][CT]GG
EaeI 1611 TGGCCG [CT]^GGCC[GA]
EcoNI 2061 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1866 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1909 GGTGACC G^GT[ACGT]ACC
EcoRI 601 GAATTC G^AATTC
FauI 1123 GCGGG CCCGC(4/6)
FbaI 678 TGATCA T^GATCA
HaeII 61 AGCGCC [GA]GCGC^[CT]
HphI 1909 GGTGA GGTGA(8/7)
MfeI 1736 CAATTG C^AATTG
MslI 472 CACGCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1736 CAATTG C^AATTG
NcoI 1118 CCATGG C^CATGG
NspV 2165 TTCGAA TT^CGAA
PaeR7I 40 CTCGAG C^TCGAG
PflMI 687 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1866 GGGACCT [GA]G^G[AT]CC[CT]
SacI 379 GAGCTC GAGCT^C
SapI 1920 GCTCTTC GCTCTTC(1/4)
SfuI 2165 TTCGAA TT^CGAA
SphI 929 GCATGC GCATG^C
SspBI 779 TGTACA T^GTACA
SspI 979 AATATT AAT^ATT
Van91I 687 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 651 TCTAGA T^CTAGA
XhoI 40 CTCGAG C^TCGAG
XmnI 1917 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 113 GGCGTC G[GA]^CG[CT]C
804 GACGTC
AlwNI 886 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2077 CAGCCACTG
BanI 1 GGTGCC G^G[CT][GA]CC
1279 GGCACC
BsaAI 1504 TACGTG [CT]AC^GT[GA]
2330 TACGTG
BsaHI 113 GGCGTC G[GA]^CG[CT]C
804 GACGTC
BseRI 493 CTCCTC GAGGAG(10/8)
45 GAGGAG
BsgI 1590 CTGCAC GTGCAG(16/14)
320 GTGCAG
BsmFI 1077 GGGAC GGGAC(10/14)
1866 GGGAC
BspMI 83 ACCTGC ACCTGC(4/8)
815 ACCTGC
DraI 2529 TTTAAA TTT^AAA
2550 TTTAAA
EcoT14I 791 CCAAGG C^C[AT][AT]GG
1118 CCATGG
HaeIII 306 GGCC GG^CC
1612 GGCC
HgaI 611 GACGC GACGC(5/10)
114 GCGTC
Hin1I 113 GGCGTC G[GA]^CG[CT]C
804 GACGTC
NdeI 635 CATATG CA^TATG
709 CATATG
PstI 331 CTGCAG CTGCA^G
2074 CTGCAG
ScaI 1061 AGTACT AGT^ACT
1158 AGTACT
SexAI 826 ACCTGGT A^CC[AT]GGT
1905 ACCTGGT
SmlI 40 CTCGAG C^T[CT][GA]AG
606 CTTAAG
StyI 791 CCAAGG C^C[AT][AT]GG
1118 CCATGG
XcmI 5 CCAGGCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1248 CCATCAGAGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AspI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmBI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII