################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AccI AccII AccIII AflII AgeI AhdI AscI AseI AsnI AspEI AvrII BamHI BbrPI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco52I FseI KspI MluI MroI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacI SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AclI 3845 AACGTT AA^CGTT AflIII 2085 ACATGT A^C[GA][CT]GT Alw44I 790 GTGCAC G^TGCAC AocI 897 CCTCAGG CC^T[ACGT]AGG Aor51HI 274 AGCGCT AGC^GCT ApaLI 790 GTGCAC G^TGCAC AspI 2934 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1828 CCCGGG C^[CT]CG[GA]G BalI 3607 TGGCCA TGG^CCA BbeI 1660 GGCGCC GGCGC^C BbsI 1561 GAAGAC GAAGAC(2/6) BbvCI 153 GCTGAGG CCTCAGC(-5/-2) BclI 1289 TGATCA T^GATCA BglI 1818 GCCAAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1561 GAAGAC GAAGAC(2/6) BsaBI 808 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 55 GAGACC GGTCTC(1/5) BsaWI 317 ACCGGA [AT]^CCGG[AT] BsmI 189 GCATTC GAATGC(1/-1) BsoBI 1828 CCCGGG C^[CT]CG[GA]G BspLU11I 2085 ACATGT A^CATGT BsrBI 1749 GAGCGG CCGCTC(-3/-3) BstEII 75 GGTTACC G^GT[ACGT]ACC BstPI 75 GGTTACC G^GT[ACGT]ACC BstXI 1007 CCAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 897 CCTCAGG CC^T[ACGT]AGG DraIII 4104 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 392 GACGTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 274 AGCGCT AGC^GCT Eco81I 897 CCTCAGG CC^T[ACGT]AGG EcoO65I 75 GGTTACC G^GT[ACGT]ACC EcoRI 2417 GAATTC G^AATTC EcoRV 1020 GATATC GAT^ATC EcoT22I 861 ATGCAT ATGCA^T FbaI 1289 TGATCA T^GATCA HpaI 3692 GTTAAC GTT^AAC KasI 1660 GGCGCC G^GCGCC MamI 808 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1239 CAATTG C^AATTG MluNI 3607 TGGCCA TGG^CCA MscI 3607 TGGCCA TGG^CCA MunI 1239 CAATTG C^AATTG NarI 1660 GGCGCC GG^CGCC NsiI 861 ATGCAT ATGCA^T PflFI 2934 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 3845 AACGTT AA^CGTT SbfI 2262 CCTGCAGG CCTGCA^GG SfoI 1660 GGCGCC GGC^GCC SmaI 1828 CCCGGG CCC^GGG Sse8387I 2262 CCTGCAGG CCTGCA^GG SspI 3935 AATATT AAT^ATT Tth111I 2934 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1828 CCCGGG C^CCGGG XmaI 1828 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 392 GACGTC GACGT^C 2170 GACGTC Acc65I 417 GGTACC G^GTACC 2268 GGTACC ApaI 1825 GGGCCC GGGCC^C 3972 GGGCCC Asp718I 417 GGTACC G^GTACC 2268 GGTACC AviII 1795 TGCGCA TGC^GCA 1873 TGCGCA BsgI 210 GTGCAG GTGCAG(16/14) 3571 GTGCAG Bsp1407I 4325 TGTACA T^GTACA 4582 TGTACA BspHI 1024 TCATGA T^CATGA 3577 TCATGA BsrDI 870 GCAATG GCAATG(2/0) 4251 GCAATG BsrGI 4325 TGTACA T^GTACA 4582 TGTACA EcoNI 3877 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4459 CCTGGACAAGG FspI 1795 TGCGCA TGC^GCA 1873 TGCGCA HindIII 1001 AAGCTT A^AGCTT 2040 AAGCTT KpnI 417 GGTACC GGTAC^C 2268 GGTACC NcoI 3191 CCATGG C^CATGG 4122 CCATGG NdeI 1077 CATATG CA^TATG 4016 CATATG PflMI 1332 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2123 CCAGAAGCTGG PvuII 4399 CAGCTG CAG^CTG 4410 CAGCTG RcaI 1024 TCATGA T^CATGA 3577 TCATGA SexAI 413 ACCAGGT A^CC[AT]GGT 1430 ACCAGGT SmlI 439 CTCAAG C^T[CT][GA]AG 4316 CTCAAG SspBI 4325 TGTACA T^GTACA 4582 TGTACA Tsp45I 3187 GTGAC ^GT[GC]AC 3238 GTCAC Van91I 1332 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2123 CCAGAAGCTGG XcmI 2101 CCACGCTGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3687 CCATTGTTAACGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI