##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AccII AccIII AflII AgeI
AhdI AscI AseI AsnI AspEI
AvrII BamHI BbrPI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I FseI KspI MluI MroI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacI SacII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 3845 AACGTT AA^CGTT
AflIII 2085 ACATGT A^C[GA][CT]GT
Alw44I 790 GTGCAC G^TGCAC
AocI 897 CCTCAGG CC^T[ACGT]AGG
Aor51HI 274 AGCGCT AGC^GCT
ApaLI 790 GTGCAC G^TGCAC
AspI 2934 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1828 CCCGGG C^[CT]CG[GA]G
BalI 3607 TGGCCA TGG^CCA
BbeI 1660 GGCGCC GGCGC^C
BbsI 1561 GAAGAC GAAGAC(2/6)
BbvCI 153 GCTGAGG CCTCAGC(-5/-2)
BclI 1289 TGATCA T^GATCA
BglI 1818 GCCAAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1561 GAAGAC GAAGAC(2/6)
BsaBI 808 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 55 GAGACC GGTCTC(1/5)
BsaWI 317 ACCGGA [AT]^CCGG[AT]
BsmI 189 GCATTC GAATGC(1/-1)
BsoBI 1828 CCCGGG C^[CT]CG[GA]G
BspLU11I 2085 ACATGT A^CATGT
BsrBI 1749 GAGCGG CCGCTC(-3/-3)
BstEII 75 GGTTACC G^GT[ACGT]ACC
BstPI 75 GGTTACC G^GT[ACGT]ACC
BstXI 1007 CCAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 897 CCTCAGG CC^T[ACGT]AGG
DraIII 4104 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 392 GACGTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 274 AGCGCT AGC^GCT
Eco81I 897 CCTCAGG CC^T[ACGT]AGG
EcoO65I 75 GGTTACC G^GT[ACGT]ACC
EcoRI 2417 GAATTC G^AATTC
EcoRV 1020 GATATC GAT^ATC
EcoT22I 861 ATGCAT ATGCA^T
FbaI 1289 TGATCA T^GATCA
HpaI 3692 GTTAAC GTT^AAC
KasI 1660 GGCGCC G^GCGCC
MamI 808 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1239 CAATTG C^AATTG
MluNI 3607 TGGCCA TGG^CCA
MscI 3607 TGGCCA TGG^CCA
MunI 1239 CAATTG C^AATTG
NarI 1660 GGCGCC GG^CGCC
NsiI 861 ATGCAT ATGCA^T
PflFI 2934 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3845 AACGTT AA^CGTT
SbfI 2262 CCTGCAGG CCTGCA^GG
SfoI 1660 GGCGCC GGC^GCC
SmaI 1828 CCCGGG CCC^GGG
Sse8387I 2262 CCTGCAGG CCTGCA^GG
SspI 3935 AATATT AAT^ATT
Tth111I 2934 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1828 CCCGGG C^CCGGG
XmaI 1828 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 392 GACGTC GACGT^C
2170 GACGTC
Acc65I 417 GGTACC G^GTACC
2268 GGTACC
ApaI 1825 GGGCCC GGGCC^C
3972 GGGCCC
Asp718I 417 GGTACC G^GTACC
2268 GGTACC
AviII 1795 TGCGCA TGC^GCA
1873 TGCGCA
BsgI 210 GTGCAG GTGCAG(16/14)
3571 GTGCAG
Bsp1407I 4325 TGTACA T^GTACA
4582 TGTACA
BspHI 1024 TCATGA T^CATGA
3577 TCATGA
BsrDI 870 GCAATG GCAATG(2/0)
4251 GCAATG
BsrGI 4325 TGTACA T^GTACA
4582 TGTACA
EcoNI 3877 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4459 CCTGGACAAGG
FspI 1795 TGCGCA TGC^GCA
1873 TGCGCA
HindIII 1001 AAGCTT A^AGCTT
2040 AAGCTT
KpnI 417 GGTACC GGTAC^C
2268 GGTACC
NcoI 3191 CCATGG C^CATGG
4122 CCATGG
NdeI 1077 CATATG CA^TATG
4016 CATATG
PflMI 1332 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2123 CCAGAAGCTGG
PvuII 4399 CAGCTG CAG^CTG
4410 CAGCTG
RcaI 1024 TCATGA T^CATGA
3577 TCATGA
SexAI 413 ACCAGGT A^CC[AT]GGT
1430 ACCAGGT
SmlI 439 CTCAAG C^T[CT][GA]AG
4316 CTCAAG
SspBI 4325 TGTACA T^GTACA
4582 TGTACA
Tsp45I 3187 GTGAC ^GT[GC]AC
3238 GTCAC
Van91I 1332 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2123 CCAGAAGCTGG
XcmI 2101 CCACGCTGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3687 CCATTGTTAACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI