##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AviII AvrII BalI BamHI
BbrPI BbvCI BclI BlnI BsaAI
BseAI BsiWI BspDI BspEI BspLU11I
BsrBI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I Eco47III EcoRV
FbaI FseI FspI MfeI MluNI
MroI MscI MunI NdeI NheI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 3003 GAATTC [GA]^AATT[CT]
AflII 1530 CTTAAG C^TTAAG
AgeI 48 ACCGGT A^CCGGT
AocI 2691 CCTCAGG CC^T[ACGT]AGG
ApaI 599 GGGCCC GGGCC^C
ApoI 3003 GAATTC [GA]^AATT[CT]
Asp700I 3030 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 804 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 218 GGCGCC GGCGC^C
BcgI 392 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1530 CTTAAG C^TTAAG
BglII 3734 AGATCT A^GATCT
BlpI 992 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 992 GCTGAGC GC^T[ACGT]AGC
BsaBI 783 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2564 GAGACC GGTCTC(1/5)
BsmBI 3548 GAGACG CGTCTC(1/5)
Bsp1407I 959 TGTACA T^GTACA
BspHI 2095 TCATGA T^CATGA
BsrGI 959 TGTACA T^GTACA
BstXI 1947 CCAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2691 CCTCAGG CC^T[ACGT]AGG
CelII 992 GCTGAGC GC^T[ACGT]AGC
DrdI 1773 GACAGAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2691 CCTCAGG CC^T[ACGT]AGG
EcoNI 541 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3003 GAATTC G^AATTC
HpaI 2466 GTTAAC GTT^AAC
KasI 218 GGCGCC G^GCGCC
MaeII 3551 ACGT A^CGT
MamI 783 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 218 GGCGCC GG^CGCC
NcoI 438 CCATGG C^CATGG
NotI 448 GCGGCCGC GC^GGCCGC
PflFI 804 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 48 ACCGGT A^CCGGT
PvuII 2787 CAGCTG CAG^CTG
RcaI 2095 TCATGA T^CATGA
SapI 1410 GCTCTTC GCTCTTC(1/4)
SfoI 218 GGCGCC GGC^GCC
SpeI 3897 ACTAGT A^CTAGT
SspBI 959 TGTACA T^GTACA
SspI 1215 AATATT AAT^ATT
StuI 831 AGGCCT AGG^CCT
TaqI 1959 TCGA T^CGA
Tth111I 804 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1959 TCGA T^CGA
XbaI 1309 TCTAGA T^CTAGA
XcmI 429 CCAGCGTATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3030 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 886 GGTACC G^GTACC
1419 GGTACC
AflIII 170 ACGCGT A^C[GA][CT]GT
290 ACGCGT
Asp718I 886 GGTACC G^GTACC
1419 GGTACC
BbsI 2663 GAAGAC GAAGAC(2/6)
3450 GAAGAC
BciVI 434 GTATCC GTATCC(6/5)
2268 GTATCC
BpuAI 2663 GAAGAC GAAGAC(2/6)
3450 GAAGAC
BsiEI 449 CGGCCG CG[GA][CT]^CG
2376 CGGCCG
BsrDI 581 CATTGC GCAATG(2/0)
2982 CATTGC
BssHII 274 GCGCGC G^CGCGC
362 GCGCGC
BstEII 916 GGTCACC G^GT[ACGT]ACC
1590 GGTGACC
BstPI 916 GGTCACC G^GT[ACGT]ACC
1590 GGTGACC
DraI 3491 TTTAAA TTT^AAA
3643 TTTAAA
EagI 449 CGGCCG C^GGCCG
2376 CGGCCG
EclXI 449 CGGCCG C^GGCCG
2376 CGGCCG
Eco52I 449 CGGCCG C^GGCCG
2376 CGGCCG
EcoO65I 916 GGTCACC G^GT[ACGT]ACC
1590 GGTGACC
EcoT14I 438 CCATGG C^C[AT][AT]GG
778 CCAAGG
FauI 101 GCGGG CCCGC(4/6)
3389 GCGGG
HaeII 218 GGCGCC [GA]GCGC^[CT]
2445 GGCGCT
HgaI 552 GCGTC GACGC(5/10)
3191 GCGTC
HincII 2466 GTTAAC GT[CT]^[GA]AC
3193 GTCAAC
HindII 2466 GTTAAC GT[CT]^[GA]AC
3193 GTCAAC
KpnI 886 GGTACC GGTAC^C
1419 GGTACC
KspI 225 CCGCGG CCGC^GG
452 CCGCGG
MluI 170 ACGCGT A^CGCGT
290 ACGCGT
NaeI 120 GCCGGC GCC^GGC
2378 GCCGGC
NgoMIV 120 GCCGGC G^CCGGC
2378 GCCGGC
PflMI 484 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4110 CCAGGGAGTGG
PpuMI 1925 GGGACCT [GA]G^G[AT]CC[CT]
2950 AGGACCC
PstI 545 CTGCAG CTGCA^G
2790 CTGCAG
SacI 1431 GAGCTC GAGCT^C
2957 GAGCTC
SacII 225 CCGCGG CCGC^GG
452 CCGCGG
SexAI 332 ACCTGGT A^CC[AT]GGT
477 ACCAGGT
SphI 2686 GCATGC GCATG^C
3137 GCATGC
StyI 438 CCATGG C^C[AT][AT]GG
778 CCAAGG
Van91I 484 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4110 CCAGGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII