##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AclI AgeI ApaI AscI
AviII BciVI BclI BglI BsiWI
Bsp1407I BspDI BsrGI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI EcoRV
FbaI FseI FspI HpaI KspI
MfeI MluI MunI NdeI NotI
NruI NspV PacI PinAI PmeI
Psp1406I RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 620 CTTAAG C^TTAAG
AhdI 1275 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2985 GTGCAC G^TGCAC
ApaLI 2985 GTGCAC G^TGCAC
AseI 4197 ATTAAT AT^TAAT
AsnI 4197 ATTAAT AT^TAAT
AspEI 1275 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4584 GGATCC G^GATCC
BbvCI 4336 GCTGAGG CCTCAGC(-5/-2)
BfrI 620 CTTAAG C^TTAAG
BsaBI 3458 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5928 GCATTC GAATGC(1/-1)
BspHI 2433 TCATGA T^CATGA
BspLU11I 4728 ACATGT A^CATGT
BsrFI 5138 GCCGGC [GA]^CCGG[CT]
BstEII 2597 GGTAACC G^GT[ACGT]ACC
BstPI 2597 GGTAACC G^GT[ACGT]ACC
Cfr10I 5138 GCCGGC [GA]^CCGG[CT]
DraIII 3897 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 28 CGGCCG C^GGCCG
Eam1105I 1275 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 28 CGGCCG C^GGCCG
Eco52I 28 CGGCCG C^GGCCG
EcoO65I 2597 GGTAACC G^GT[ACGT]ACC
EcoRI 407 GAATTC G^AATTC
HincII 1266 GTTGAC GT[CT]^[GA]AC
HindII 1266 GTTGAC GT[CT]^[GA]AC
HindIII 4680 AAGCTT A^AGCTT
MamI 3458 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5138 GCCGGC GCC^GGC
NgoMIV 5138 GCCGGC G^CCGGC
NheI 3074 GCTAGC G^CTAGC
PaeR7I 2604 CTCGAG C^TCGAG
PshBI 4197 ATTAAT AT^TAAT
PvuI 32 CGATCG CGAT^CG
RcaI 2433 TCATGA T^CATGA
SacI 1881 GAGCTC GAGCT^C
SnaBI 4962 TACGTA TAC^GTA
SpeI 5980 ACTAGT A^CTAGT
XbaI 2069 TCTAGA T^CTAGA
XhoI 2604 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3370 GGTACC G^GTACC
3873 GGTACC
AccI 828 GTAGAC GT^[AC][GT]AC
5215 GTCTAC
AccIII 928 TCCGGA T^CCGGA
1408 TCCGGA
AcyI 1655 GGCGCC G[GA]^CG[CT]C
5636 GGCGCC
AocI 4621 CCTTAGG CC^T[ACGT]AGG
5542 CCTTAGG
Aor51HI 20 AGCGCT AGC^GCT
2649 AGCGCT
Asp718I 3370 GGTACC G^GTACC
3873 GGTACC
AspI 990 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5146 GACTGTGTC
AvrII 297 CCTAGG C^CTAGG
3492 CCTAGG
BbeI 1655 GGCGCC GGCGC^C
5636 GGCGCC
BbrPI 2500 CACGTG CAC^GTG
4720 CACGTG
BglII 1594 AGATCT A^GATCT
2824 AGATCT
BlnI 297 CCTAGG C^CTAGG
3492 CCTAGG
BsaHI 1655 GGCGCC G[GA]^CG[CT]C
5636 GGCGCC
BseAI 928 TCCGGA T^CCGGA
1408 TCCGGA
BsiEI 28 CGGCCG CG[GA][CT]^CG
32 CGATCG
BspEI 928 TCCGGA T^CCGGA
1408 TCCGGA
BsrBI 1304 CCGCTC CCGCTC(-3/-3)
3579 GAGCGG
BssHII 211 GCGCGC G^CGCGC
231 GCGCGC
BssSI 3000 CTCGTG CACGAG(-5/-1)
5307 CTCGTG
Bsu36I 4621 CCTTAGG CC^T[ACGT]AGG
5542 CCTTAGG
DrdI 987 GACGACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1455 GACTCGCGGGTC
Eco47III 20 AGCGCT AGC^GCT
2649 AGCGCT
Eco81I 4621 CCTTAGG CC^T[ACGT]AGG
5542 CCTTAGG
EcoNI 3682 CCTATTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5542 CCTTAGGCAGG
HgaI 6 GCGTC GACGC(5/10)
51 GCGTC
Hin1I 1655 GGCGCC G[GA]^CG[CT]C
5636 GGCGCC
KasI 1655 GGCGCC G^GCGCC
5636 GGCGCC
KpnI 3370 GGTACC GGTAC^C
3873 GGTACC
MroI 928 TCCGGA T^CCGGA
1408 TCCGGA
NarI 1655 GGCGCC GG^CGCC
5636 GGCGCC
PflFI 990 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5146 GACTGTGTC
PflMI 1556 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2758 CCATCTCCTGG
PmaCI 2500 CACGTG CAC^GTG
4720 CACGTG
PmlI 2500 CACGTG CAC^GTG
4720 CACGTG
PshAI 2498 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4086 GACTGGAGTC
SexAI 991 ACCAGGT A^CC[AT]GGT
3186 ACCAGGT
SfoI 1655 GGCGCC GGC^GCC
5636 GGCGCC
SspI 2487 AATATT AAT^ATT
2895 AATATT
StuI 1995 AGGCCT AGG^CCT
3208 AGGCCT
Tth111I 990 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5146 GACTGTGTC
Van91I 1556 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2758 CCATCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI