################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AclI AgeI ApaI AscI AviII BciVI BclI BglI BsiWI Bsp1407I BspDI BsrGI Bst1107I BstBI BstXI BstZ17I ClaI CpoI EcoRV FbaI FseI FspI HpaI KspI MfeI MluI MunI NdeI NotI NruI NspV PacI PinAI PmeI Psp1406I RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 620 CTTAAG C^TTAAG AhdI 1275 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2985 GTGCAC G^TGCAC ApaLI 2985 GTGCAC G^TGCAC AseI 4197 ATTAAT AT^TAAT AsnI 4197 ATTAAT AT^TAAT AspEI 1275 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 4584 GGATCC G^GATCC BbvCI 4336 GCTGAGG CCTCAGC(-5/-2) BfrI 620 CTTAAG C^TTAAG BsaBI 3458 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 5928 GCATTC GAATGC(1/-1) BspHI 2433 TCATGA T^CATGA BspLU11I 4728 ACATGT A^CATGT BsrFI 5138 GCCGGC [GA]^CCGG[CT] BstEII 2597 GGTAACC G^GT[ACGT]ACC BstPI 2597 GGTAACC G^GT[ACGT]ACC Cfr10I 5138 GCCGGC [GA]^CCGG[CT] DraIII 3897 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 28 CGGCCG C^GGCCG Eam1105I 1275 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 28 CGGCCG C^GGCCG Eco52I 28 CGGCCG C^GGCCG EcoO65I 2597 GGTAACC G^GT[ACGT]ACC EcoRI 407 GAATTC G^AATTC HincII 1266 GTTGAC GT[CT]^[GA]AC HindII 1266 GTTGAC GT[CT]^[GA]AC HindIII 4680 AAGCTT A^AGCTT MamI 3458 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 5138 GCCGGC GCC^GGC NgoMIV 5138 GCCGGC G^CCGGC NheI 3074 GCTAGC G^CTAGC PaeR7I 2604 CTCGAG C^TCGAG PshBI 4197 ATTAAT AT^TAAT PvuI 32 CGATCG CGAT^CG RcaI 2433 TCATGA T^CATGA SacI 1881 GAGCTC GAGCT^C SnaBI 4962 TACGTA TAC^GTA SpeI 5980 ACTAGT A^CTAGT XbaI 2069 TCTAGA T^CTAGA XhoI 2604 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 3370 GGTACC G^GTACC 3873 GGTACC AccI 828 GTAGAC GT^[AC][GT]AC 5215 GTCTAC AccIII 928 TCCGGA T^CCGGA 1408 TCCGGA AcyI 1655 GGCGCC G[GA]^CG[CT]C 5636 GGCGCC AocI 4621 CCTTAGG CC^T[ACGT]AGG 5542 CCTTAGG Aor51HI 20 AGCGCT AGC^GCT 2649 AGCGCT Asp718I 3370 GGTACC G^GTACC 3873 GGTACC AspI 990 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5146 GACTGTGTC AvrII 297 CCTAGG C^CTAGG 3492 CCTAGG BbeI 1655 GGCGCC GGCGC^C 5636 GGCGCC BbrPI 2500 CACGTG CAC^GTG 4720 CACGTG BglII 1594 AGATCT A^GATCT 2824 AGATCT BlnI 297 CCTAGG C^CTAGG 3492 CCTAGG BsaHI 1655 GGCGCC G[GA]^CG[CT]C 5636 GGCGCC BseAI 928 TCCGGA T^CCGGA 1408 TCCGGA BsiEI 28 CGGCCG CG[GA][CT]^CG 32 CGATCG BspEI 928 TCCGGA T^CCGGA 1408 TCCGGA BsrBI 1304 CCGCTC CCGCTC(-3/-3) 3579 GAGCGG BssHII 211 GCGCGC G^CGCGC 231 GCGCGC BssSI 3000 CTCGTG CACGAG(-5/-1) 5307 CTCGTG Bsu36I 4621 CCTTAGG CC^T[ACGT]AGG 5542 CCTTAGG DrdI 987 GACGACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1455 GACTCGCGGGTC Eco47III 20 AGCGCT AGC^GCT 2649 AGCGCT Eco81I 4621 CCTTAGG CC^T[ACGT]AGG 5542 CCTTAGG EcoNI 3682 CCTATTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5542 CCTTAGGCAGG HgaI 6 GCGTC GACGC(5/10) 51 GCGTC Hin1I 1655 GGCGCC G[GA]^CG[CT]C 5636 GGCGCC KasI 1655 GGCGCC G^GCGCC 5636 GGCGCC KpnI 3370 GGTACC GGTAC^C 3873 GGTACC MroI 928 TCCGGA T^CCGGA 1408 TCCGGA NarI 1655 GGCGCC GG^CGCC 5636 GGCGCC PflFI 990 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5146 GACTGTGTC PflMI 1556 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2758 CCATCTCCTGG PmaCI 2500 CACGTG CAC^GTG 4720 CACGTG PmlI 2500 CACGTG CAC^GTG 4720 CACGTG PshAI 2498 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4086 GACTGGAGTC SexAI 991 ACCAGGT A^CC[AT]GGT 3186 ACCAGGT SfoI 1655 GGCGCC GGC^GCC 5636 GGCGCC SspI 2487 AATATT AAT^ATT 2895 AATATT StuI 1995 AGGCCT AGG^CCT 3208 AGGCCT Tth111I 990 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5146 GACTGTGTC Van91I 1556 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2758 CCATCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI XmnI