##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AccIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BcgI BciVI BsaBI BseAI BsiWI
BspDI BspEI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
Eam1105I FseI FspI KpnI MamI
MluI MroI NaeI NdeI NgoMIV
NheI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI PvuI
RsrII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 3112 GACGTC GACGT^C
AclI 1844 AACGTT AA^CGTT
AflII 4873 CTTAAG C^TTAAG
Aor51HI 3230 AGCGCT AGC^GCT
AvaI 2759 CCCGAG C^[CT]CG[GA]G
AvrII 3388 CCTAGG C^CTAGG
BalI 3854 TGGCCA TGG^CCA
BamHI 1159 GGATCC G^GATCC
BbrPI 3345 CACGTG CAC^GTG
BbsI 4501 GTCTTC GAAGAC(2/6)
BbvCI 689 CCTCAGC CCTCAGC(-5/-2)
BcnI 24 CCGGG CC^[GC]GG
BfrI 4873 CTTAAG C^TTAAG
BglII 512 AGATCT A^GATCT
BlnI 3388 CCTAGG C^CTAGG
BpmI 2820 CTGGAG CTGGAG(16/14)
BpuAI 4501 GTCTTC GAAGAC(2/6)
BsaAI 3345 CACGTG [CT]AC^GT[GA]
BsaI 4380 GAGACC GGTCTC(1/5)
BsaWI 2298 ACCGGA [AT]^CCGG[AT]
BsiEI 60 CGGCCG CG[GA][CT]^CG
BsmBI 3368 CGTCTC CGTCTC(1/5)
BsoBI 2759 CCCGAG C^[CT]CG[GA]G
BsrBI 2554 GAGCGG CCGCTC(-3/-3)
BsrDI 429 CATTGC GCAATG(2/0)
BssSI 2743 CTCGTG CACGAG(-5/-1)
DraIII 4915 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 60 CGGCCG C^GGCCG
EclXI 60 CGGCCG C^GGCCG
Eco47III 3230 AGCGCT AGC^GCT
Eco52I 60 CGGCCG C^GGCCG
EcoRI 2271 GAATTC G^AATTC
EcoRV 4977 GATATC GAT^ATC
EcoT22I 3859 ATGCAT ATGCA^T
HincII 4203 GTTAAC GT[CT]^[GA]AC
HindII 4203 GTTAAC GT[CT]^[GA]AC
HpaI 4203 GTTAAC GTT^AAC
MfeI 5046 CAATTG C^AATTG
MluNI 3854 TGGCCA TGG^CCA
MscI 3854 TGGCCA TGG^CCA
MunI 5046 CAATTG C^AATTG
NciI 24 CCGGG CC^[GC]GG
NotI 59 GCGGCCGC GC^GGCCGC
NsiI 3859 ATGCAT ATGCA^T
PflMI 2216 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3345 CACGTG CAC^GTG
PmlI 3345 CACGTG CAC^GTG
PpuMI 685 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 680 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1844 AACGTT AA^CGTT
PstI 2006 CTGCAG CTGCA^G
SacI 3682 GAGCTC GAGCT^C
Van91I 2216 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 1630 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2009 CAGATTCTG
AocI 355 CCTGAGG CC^T[ACGT]AGG
3463 CCTCAGG
BanI 659 GGCGCC G^G[CT][GA]CC
1600 GGCGCC
BbeI 659 GGCGCC GGCGC^C
1600 GGCGCC
BclI 4168 TGATCA T^GATCA
4392 TGATCA
BseRI 281 GAGGAG GAGGAG(10/8)
2997 GAGGAG
BspMI 193 GCAGGT ACCTGC(4/8)
2700 GCAGGT
BstEII 3648 GGTCACC G^GT[ACGT]ACC
3907 GGTTACC
BstPI 3648 GGTCACC G^GT[ACGT]ACC
3907 GGTTACC
BstXI 1705 CCATGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1816 CCATGACCCTGG
Bsu36I 355 CCTGAGG CC^T[ACGT]AGG
3463 CCTCAGG
DraII 351 AGGGCCT [GA]G^G[ACGT]CC[CT]
685 AGGTCCT
DrdI 338 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1820 GACCCTGGGGTC
Eco81I 355 CCTGAGG CC^T[ACGT]AGG
3463 CCTCAGG
EcoO109I 351 AGGGCCT [GA]G^G[ACGT]CC[CT]
685 AGGTCCT
EcoO65I 3648 GGTCACC G^GT[ACGT]ACC
3907 GGTTACC
FbaI 4168 TGATCA T^GATCA
4392 TGATCA
HgaI 2559 GACGC GACGC(5/10)
1444 GCGTC
KasI 659 GGCGCC G^GCGCC
1600 GGCGCC
KspI 57 CCGCGG CCGC^GG
66 CCGCGG
MslI 881 CACACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4838 CACAAATGTG
NarI 659 GGCGCC GG^CGCC
1600 GGCGCC
PvuII 1164 CAGCTG CAG^CTG
1633 CAGCTG
SacII 57 CCGCGG CCGC^GG
66 CCGCGG
SexAI 2950 ACCTGGT A^CC[AT]GGT
3903 ACCAGGT
SfoI 659 GGCGCC GGC^GCC
1600 GGCGCC
StuI 2763 AGGCCT AGG^CCT
4599 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaII BanII BbvI BfaI
BglI BlpI BmyI Bpu1102I BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI