##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccI AclI AgeI AscI
AseI AsnI AspI AvrII BcgI
BciVI BglII BlnI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
FseI HpaI KspI MluI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfuI SgrAI Sse8387I SspBI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 5904 GGTACC G^GTACC
AflIII 5251 ACACGT A^C[GA][CT]GT
Alw44I 6188 GTGCAC G^TGCAC
AocI 5847 CCTAAGG CC^T[ACGT]AGG
ApaLI 6188 GTGCAC G^TGCAC
Asp718I 5904 GGTACC G^GTACC
BbrPI 5252 CACGTG CAC^GTG
BsaBI 1692 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 6757 CGACCG CG[GA][CT]^CG
BstEII 6124 GGTGACC G^GT[ACGT]ACC
BstPI 6124 GGTGACC G^GT[ACGT]ACC
Bsu36I 5847 CCTAAGG CC^T[ACGT]AGG
DraI 1920 TTTAAA TTT^AAA
DraIII 1648 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 5847 CCTAAGG CC^T[ACGT]AGG
EcoO65I 6124 GGTGACC G^GT[ACGT]ACC
EcoRV 1933 GATATC GAT^ATC
EcoT22I 60 ATGCAT ATGCA^T
HindIII 6800 AAGCTT A^AGCTT
KpnI 5904 GGTACC GGTAC^C
MamI 1692 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5679 CAATTG C^AATTG
MunI 5679 CAATTG C^AATTG
NaeI 5396 GCCGGC GCC^GGC
NcoI 557 CCATGG C^CATGG
NgoMIV 5396 GCCGGC G^CCGGC
NsiI 60 ATGCAT ATGCA^T
PmaCI 5252 CACGTG CAC^GTG
PmlI 5252 CACGTG CAC^GTG
PshAI 5149 GACGAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 6612 AGTACT AGT^ACT
SexAI 5952 ACCTGGT A^CC[AT]GGT
SfiI 4281 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 790 TACGTA TAC^GTA
SpeI 5097 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 308 TCCGGA T^CCGGA
1694 TCCGGA
AflII 4354 CTTAAG C^TTAAG
5630 CTTAAG
AhdI 2347 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2947 GACTTTGAGTC
ApaI 2462 GGGCCC GGGCC^C
4040 GGGCCC
AspEI 2347 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2947 GACTTTGAGTC
AviII 5647 TGCGCA TGC^GCA
6712 TGCGCA
BbeI 4766 GGCGCC GGCGC^C
5399 GGCGCC
BclI 4499 TGATCA T^GATCA
6015 TGATCA
BfrI 4354 CTTAAG C^TTAAG
5630 CTTAAG
BsaAI 790 TACGTA [CT]AC^GT[GA]
5252 CACGTG
BseAI 308 TCCGGA T^CCGGA
1694 TCCGGA
BsmI 1183 GAATGC GAATGC(1/-1)
6550 GCATTC
BspEI 308 TCCGGA T^CCGGA
1694 TCCGGA
BstXI 1556 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3176 CCACAGCGCTGG
DsaI 557 CCATGG C^C[GA][CT]GG
6760 CCGTGG
Eam1105I 2347 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2947 GACTTTGAGTC
EcoNI 1050 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4044 CCTTGAGGAGG
FbaI 4499 TGATCA T^GATCA
6015 TGATCA
FspI 5647 TGCGCA TGC^GCA
6712 TGCGCA
KasI 4766 GGCGCC G^GCGCC
5399 GGCGCC
MroI 308 TCCGGA T^CCGGA
1694 TCCGGA
MslI 1464 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2085 CACGAAGGTG
NarI 4766 GGCGCC GG^CGCC
5399 GGCGCC
SfoI 4766 GGCGCC GGC^GCC
5399 GGCGCC
SspI 196 AATATT AAT^ATT
6396 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco47III
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI