##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII Aor51HI ApaI AscI AviII
BbeI BcgI BciVI BclI BglI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiWI BspDI BspEI BssHII BstBI
BstEII BstPI CelII ClaI EagI
EclXI Eco47III Eco52I EcoO65I FbaI
FseI FspI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PaeR7I PmeI PpuMI PshAI
PvuI SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I StuI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 1593 GACGTC GACGT^C
AcyI 1593 GACGTC G[GA]^CG[CT]C
AflII 3849 CTTAAG C^TTAAG
AgeI 1549 ACCGGT A^CCGGT
AhdI 2245 GACGGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4638 CCTGAGG CC^T[ACGT]AGG
AseI 5869 ATTAAT AT^TAAT
AsnI 5869 ATTAAT AT^TAAT
Asp700I 1511 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2245 GACGGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4891 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4709 CCCGGG C^[CT]CG[GA]G
AvrII 5085 CCTAGG C^CTAGG
BamHI 759 GGATCC G^GATCC
BbrPI 4804 CACGTG CAC^GTG
BfrI 3849 CTTAAG C^TTAAG
BglII 5291 AGATCT A^GATCT
BlnI 5085 CCTAGG C^CTAGG
BpmI 3699 CTGGAG CTGGAG(16/14)
BsaHI 1593 GACGTC G[GA]^CG[CT]C
BsiEI 1407 CGGTCG CG[GA][CT]^CG
BsoBI 4709 CCCGGG C^[CT]CG[GA]G
BsrBI 14 CCGCTC CCGCTC(-3/-3)
BsrFI 1549 ACCGGT [GA]^CCGG[CT]
Bst1107I 3760 GTATAC GTA^TAC
BstXI 4827 CCAACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3760 GTATAC GTA^TAC
Bsu36I 4638 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1549 ACCGGT [GA]^CCGG[CT]
CpoI 2288 CGGTCCG CG^G[AT]CCG
DrdI 70 GACGCTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2245 GACGGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 932 CTCTTC CTCTTC(1/4)
Eco81I 4638 CCTGAGG CC^T[ACGT]AGG
EcoRV 5519 GATATC GAT^ATC
EcoT22I 4542 ATGCAT ATGCA^T
HaeII 4579 AGCGCC [GA]GCGC^[CT]
Hin1I 1593 GACGTC G[GA]^CG[CT]C
HincII 5044 GTTAAC GT[CT]^[GA]AC
HindII 5044 GTTAAC GT[CT]^[GA]AC
HpaI 5044 GTTAAC GTT^AAC
Ksp632I 932 CTCTTC CTCTTC(1/4)
NheI 2883 GCTAGC G^CTAGC
NsiI 4542 ATGCAT ATGCA^T
PacI 720 TTAATTAA TTAAT^TAA
PflFI 4891 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2517 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1549 ACCGGT A^CCGGT
PmaCI 4804 CACGTG CAC^GTG
PmlI 4804 CACGTG CAC^GTG
PshBI 5869 ATTAAT AT^TAAT
RsrII 2288 CGGTCCG CG^G[AT]CCG
SexAI 5698 ACCAGGT A^CC[AT]GGT
SmaI 4709 CCCGGG CCC^GGG
SphI 4540 GCATGC GCATG^C
SwaI 473 ATTTAAAT ATTT^AAAT
Tth111I 4891 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2517 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4709 CCCGGG C^CCGGG
XmaI 4709 CCCGGG C^CCGGG
XmnI 1511 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2337 GGTACC G^GTACC
5572 GGTACC
AclI 1530 AACGTT AA^CGTT
2275 AACGTT
AflIII 2932 ACATGT A^C[GA][CT]GT
5469 ACATGT
AlwI 760 GATCC GGATC(4/5)
759 GGATC
Asp718I 2337 GGTACC G^GTACC
5572 GGTACC
BsaAI 2306 CACGTA [CT]AC^GT[GA]
4804 CACGTG
BsaWI 1549 ACCGGT [AT]^CCGG[AT]
3686 TCCGGT
BseRI 1077 GAGGAG GAGGAG(10/8)
4529 GAGGAG
BsgI 4801 CTGCAC GTGCAG(16/14)
1629 GTGCAG
BsmBI 2293 CGTCTC CGTCTC(1/5)
1107 GAGACG
BspHI 914 TCATGA T^CATGA
2675 TCATGA
BspLU11I 2932 ACATGT A^CATGT
5469 ACATGT
BspMI 1402 ACCTGC ACCTGC(4/8)
5569 GCAGGT
BsrDI 612 GCAATG GCAATG(2/0)
2838 GCAATG
BssSI 745 CACGAG CACGAG(-5/-1)
4488 CTCGTG
BstYI 759 GGATCC [GA]^GATC[CT]
5291 AGATCT
DraII 1471 GGGGCCT [GA]G^G[ACGT]CC[CT]
4458 AGGGCCT
DraIII 3971 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5545 CACAAAGTG
EcoNI 3455 CCTTTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4226 CCTCAGCAAGG
EcoO109I 1471 GGGGCCT [GA]G^G[ACGT]CC[CT]
4458 AGGGCCT
EcoRI 93 GAATTC G^AATTC
1718 GAATTC
FauI 3344 CCCGC CCCGC(4/6)
3485 CCCGC
KpnI 2337 GGTACC GGTAC^C
5572 GGTACC
MflI 759 GGATCC [GA]^GATC[CT]
5291 AGATCT
Psp1406I 1530 AACGTT AA^CGTT
2275 AACGTT
RcaI 914 TCATGA T^CATGA
2675 TCATGA
ScaI 2481 AGTACT AGT^ACT
5706 AGTACT
SmlI 3849 CTTAAG C^T[CT][GA]AG
4899 CTCAAG
XbaI 1073 TCTAGA T^CTAGA
5523 TCTAGA
XcmI 947 CCAGTACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1615 CCACTGAAGAAGTGG
XhoII 759 GGATCC [GA]^GATC[CT]
5291 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwNI ApaLI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SpeI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I