################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AccIII Aor51HI ApaI AscI AviII BbeI BcgI BciVI BclI BglI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BspDI BspEI BssHII BstBI BstEII BstPI CelII ClaI EagI EclXI Eco47III Eco52I EcoO65I FbaI FseI FspI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PaeR7I PmeI PpuMI PshAI PvuI SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I StuI XhoI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 1593 GACGTC GACGT^C AcyI 1593 GACGTC G[GA]^CG[CT]C AflII 3849 CTTAAG C^TTAAG AgeI 1549 ACCGGT A^CCGGT AhdI 2245 GACGGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 4638 CCTGAGG CC^T[ACGT]AGG AseI 5869 ATTAAT AT^TAAT AsnI 5869 ATTAAT AT^TAAT Asp700I 1511 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2245 GACGGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4891 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 4709 CCCGGG C^[CT]CG[GA]G AvrII 5085 CCTAGG C^CTAGG BamHI 759 GGATCC G^GATCC BbrPI 4804 CACGTG CAC^GTG BfrI 3849 CTTAAG C^TTAAG BglII 5291 AGATCT A^GATCT BlnI 5085 CCTAGG C^CTAGG BpmI 3699 CTGGAG CTGGAG(16/14) BsaHI 1593 GACGTC G[GA]^CG[CT]C BsiEI 1407 CGGTCG CG[GA][CT]^CG BsoBI 4709 CCCGGG C^[CT]CG[GA]G BsrBI 14 CCGCTC CCGCTC(-3/-3) BsrFI 1549 ACCGGT [GA]^CCGG[CT] Bst1107I 3760 GTATAC GTA^TAC BstXI 4827 CCAACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3760 GTATAC GTA^TAC Bsu36I 4638 CCTGAGG CC^T[ACGT]AGG Cfr10I 1549 ACCGGT [GA]^CCGG[CT] CpoI 2288 CGGTCCG CG^G[AT]CCG DrdI 70 GACGCTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2245 GACGGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 932 CTCTTC CTCTTC(1/4) Eco81I 4638 CCTGAGG CC^T[ACGT]AGG EcoRV 5519 GATATC GAT^ATC EcoT22I 4542 ATGCAT ATGCA^T HaeII 4579 AGCGCC [GA]GCGC^[CT] Hin1I 1593 GACGTC G[GA]^CG[CT]C HincII 5044 GTTAAC GT[CT]^[GA]AC HindII 5044 GTTAAC GT[CT]^[GA]AC HpaI 5044 GTTAAC GTT^AAC Ksp632I 932 CTCTTC CTCTTC(1/4) NheI 2883 GCTAGC G^CTAGC NsiI 4542 ATGCAT ATGCA^T PacI 720 TTAATTAA TTAAT^TAA PflFI 4891 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2517 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1549 ACCGGT A^CCGGT PmaCI 4804 CACGTG CAC^GTG PmlI 4804 CACGTG CAC^GTG PshBI 5869 ATTAAT AT^TAAT RsrII 2288 CGGTCCG CG^G[AT]CCG SexAI 5698 ACCAGGT A^CC[AT]GGT SmaI 4709 CCCGGG CCC^GGG SphI 4540 GCATGC GCATG^C SwaI 473 ATTTAAAT ATTT^AAAT Tth111I 4891 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2517 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 4709 CCCGGG C^CCGGG XmaI 4709 CCCGGG C^CCGGG XmnI 1511 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 2337 GGTACC G^GTACC 5572 GGTACC AclI 1530 AACGTT AA^CGTT 2275 AACGTT AflIII 2932 ACATGT A^C[GA][CT]GT 5469 ACATGT AlwI 760 GATCC GGATC(4/5) 759 GGATC Asp718I 2337 GGTACC G^GTACC 5572 GGTACC BsaAI 2306 CACGTA [CT]AC^GT[GA] 4804 CACGTG BsaWI 1549 ACCGGT [AT]^CCGG[AT] 3686 TCCGGT BseRI 1077 GAGGAG GAGGAG(10/8) 4529 GAGGAG BsgI 4801 CTGCAC GTGCAG(16/14) 1629 GTGCAG BsmBI 2293 CGTCTC CGTCTC(1/5) 1107 GAGACG BspHI 914 TCATGA T^CATGA 2675 TCATGA BspLU11I 2932 ACATGT A^CATGT 5469 ACATGT BspMI 1402 ACCTGC ACCTGC(4/8) 5569 GCAGGT BsrDI 612 GCAATG GCAATG(2/0) 2838 GCAATG BssSI 745 CACGAG CACGAG(-5/-1) 4488 CTCGTG BstYI 759 GGATCC [GA]^GATC[CT] 5291 AGATCT DraII 1471 GGGGCCT [GA]G^G[ACGT]CC[CT] 4458 AGGGCCT DraIII 3971 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 5545 CACAAAGTG EcoNI 3455 CCTTTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4226 CCTCAGCAAGG EcoO109I 1471 GGGGCCT [GA]G^G[ACGT]CC[CT] 4458 AGGGCCT EcoRI 93 GAATTC G^AATTC 1718 GAATTC FauI 3344 CCCGC CCCGC(4/6) 3485 CCCGC KpnI 2337 GGTACC GGTAC^C 5572 GGTACC MflI 759 GGATCC [GA]^GATC[CT] 5291 AGATCT Psp1406I 1530 AACGTT AA^CGTT 2275 AACGTT RcaI 914 TCATGA T^CATGA 2675 TCATGA ScaI 2481 AGTACT AGT^ACT 5706 AGTACT SmlI 3849 CTTAAG C^T[CT][GA]AG 4899 CTCAAG XbaI 1073 TCTAGA T^CTAGA 5523 TCTAGA XcmI 947 CCAGTACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1615 CCACTGAAGAAGTGG XhoII 759 GGATCC [GA]^GATC[CT] 5291 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI Alw44I AlwNI ApaLI ApoI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I