##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AviII AvrII BbrPI BclI BfrI
BglII BlnI BsiWI BspDI BssHII
ClaI CpoI FbaI FseI FspI
MluI NdeI NotI NruI PacI
PinAI PmaCI PmlI Psp1406I PvuI
RsrII SalI SbfI SfiI Sse8387I
SspI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1531 GGTACC G^GTACC
AccIII 202 TCCGGA T^CCGGA
AhdI 2710 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4008 GTGCAC G^TGCAC
Aor51HI 65 AGCGCT AGC^GCT
ApaLI 4008 GTGCAC G^TGCAC
AseI 6393 ATTAAT AT^TAAT
AsnI 6393 ATTAAT AT^TAAT
Asp718I 1531 GGTACC G^GTACC
AspEI 2710 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2089 GGATCC G^GATCC
BbeI 5154 GGCGCC GGCGC^C
BciVI 5237 GTATCC GTATCC(6/5)
BsaAI 3686 TACGTA [CT]AC^GT[GA]
BsaBI 285 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 202 TCCGGA T^CCGGA
Bsp1407I 5854 TGTACA T^GTACA
BspEI 202 TCCGGA T^CCGGA
BspHI 1239 TCATGA T^CATGA
BsrBI 2306 GAGCGG CCGCTC(-3/-3)
BsrGI 5854 TGTACA T^GTACA
Bst1107I 2559 GTATAC GTA^TAC
BstAPI 4822 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1009 TTCGAA TT^CGAA
BstEII 2485 GGTGACC G^GT[ACGT]ACC
BstPI 2485 GGTGACC G^GT[ACGT]ACC
BstXI 919 CCATCCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2559 GTATAC GTA^TAC
Eam1105I 2710 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 65 AGCGCT AGC^GCT
EcoO65I 2485 GGTGACC G^GT[ACGT]ACC
EcoRV 4583 GATATC GAT^ATC
EcoT22I 5611 ATGCAT ATGCA^T
KasI 5154 GGCGCC G^GCGCC
KpnI 1531 GGTACC GGTAC^C
MamI 285 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6359 CAATTG C^AATTG
MroI 202 TCCGGA T^CCGGA
MunI 6359 CAATTG C^AATTG
NarI 5154 GGCGCC GG^CGCC
NheI 5162 GCTAGC G^CTAGC
NsiI 5611 ATGCAT ATGCA^T
NspV 1009 TTCGAA TT^CGAA
PaeR7I 2232 CTCGAG C^TCGAG
PmeI 817 GTTTAAAC GTTT^AAAC
PshAI 2288 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6393 ATTAAT AT^TAAT
RcaI 1239 TCATGA T^CATGA
ScaI 1124 AGTACT AGT^ACT
SfoI 5154 GGCGCC GGC^GCC
SfuI 1009 TTCGAA TT^CGAA
SgrAI 3492 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 3686 TACGTA TAC^GTA
SphI 3090 GCATGC GCATG^C
SspBI 5854 TGTACA T^GTACA
XbaI 240 TCTAGA T^CTAGA
XhoI 2232 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2559 GTATAC GT^[AC][GT]AC
2718 GTCTAC
ApaI 25 GGGCCC GGGCC^C
5406 GGGCCC
AspI 2602 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3247 GACGGTGTC
BalI 493 TGGCCA TGG^CCA
4245 TGGCCA
BlpI 2335 GCTGAGC GC^T[ACGT]AGC
3871 GCTGAGC
Bpu1102I 2335 GCTGAGC GC^T[ACGT]AGC
3871 GCTGAGC
BsmBI 4288 CGTCTC CGTCTC(1/5)
6322 GAGACG
BspLU11I 2289 ACATGT A^CATGT
6303 ACATGT
BsrDI 1289 CATTGC GCAATG(2/0)
5844 CATTGC
BssSI 1295 CACGAG CACGAG(-5/-1)
4871 CTCGTG
CelII 2335 GCTGAGC GC^T[ACGT]AGC
3871 GCTGAGC
DraI 332 TTTAAA TTT^AAA
818 TTTAAA
DraIII 398 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
4013 CACCTGGTG
DrdI 3968 GACCAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6085 GACGCTAGTGTC
EagI 193 CGGCCG C^GGCCG
1500 CGGCCG
EclXI 193 CGGCCG C^GGCCG
1500 CGGCCG
Eco52I 193 CGGCCG C^GGCCG
1500 CGGCCG
EcoRI 809 GAATTC G^AATTC
6151 GAATTC
HindIII 559 AAGCTT A^AGCTT
1931 AAGCTT
HpaI 183 GTTAAC GTT^AAC
3862 GTTAAC
KspI 109 CCGCGG CCGC^GG
4896 CCGCGG
MluNI 493 TGGCCA TGG^CCA
4245 TGGCCA
MscI 493 TGGCCA TGG^CCA
4245 TGGCCA
PflFI 2602 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3247 GACGGTGTC
SacII 109 CCGCGG CCGC^GG
4896 CCGCGG
SapI 4478 GAAGAGC GCTCTTC(1/4)
5939 GAAGAGC
SmaI 1374 CCCGGG CCC^GGG
2553 CCCGGG
Tth111I 2602 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3247 GACGGTGTC
XmaCI 1374 CCCGGG C^CCGGG
2553 CCCGGG
XmaI 1374 CCCGGG C^CCGGG
2553 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SpeI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI