################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AclI AscI AviII AvrII BciVI BlnI BsaBI BsiEI BsiWI BspDI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoRI EcoRV FseI FspI KspI MamI MluI NheI NotI NruI NspV PacI PmeI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SfiI SfuI SgrAI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflIII 495 ACATGT A^C[GA][CT]GT AgeI 3342 ACCGGT A^CCGGT AhdI 3276 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2297 GTGCAC G^TGCAC Aor51HI 1020 AGCGCT AGC^GCT ApaI 1672 GGGCCC GGGCC^C ApaLI 2297 GTGCAC G^TGCAC AseI 1054 ATTAAT AT^TAAT AsnI 1054 ATTAAT AT^TAAT AspEI 3276 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 828 GGATCC G^GATCC BbeI 1728 GGCGCC GGCGC^C BcgI 1353 CGACCTCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2626 TGATCA T^GATCA BglII 669 AGATCT A^GATCT BsmBI 270 GAGACG CGTCTC(1/5) Bsp1407I 402 TGTACA T^GTACA BspLU11I 495 ACATGT A^CATGT BsrBI 3259 GAGCGG CCGCTC(-3/-3) BsrGI 402 TGTACA T^GTACA BstAPI 4051 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 462 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 4410 TTTAAA TTT^AAA DraIII 2961 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3146 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3276 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1020 AGCGCT AGC^GCT EcoNI 1030 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 2626 TGATCA T^GATCA KasI 1728 GGCGCC G^GCGCC NaeI 2078 GCCGGC GCC^GGC NarI 1728 GGCGCC GG^CGCC NcoI 1365 CCATGG C^CATGG NdeI 3611 CATATG CA^TATG NgoMIV 2078 GCCGGC G^CCGGC PaeR7I 419 CTCGAG C^TCGAG PflMI 2046 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3342 ACCGGT A^CCGGT PshAI 3715 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1054 ATTAAT AT^TAAT ScaI 576 AGTACT AGT^ACT SexAI 474 ACCTGGT A^CC[AT]GGT SfoI 1728 GGCGCC GGC^GCC SspBI 402 TGTACA T^GTACA StuI 1755 AGGCCT AGG^CCT Van91I 2046 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 419 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AatII 272 GACGTC GACGT^C 317 GACGTC Acc65I 335 GGTACC G^GTACC 645 GGTACC AcsI 1436 GAATTT [GA]^AATT[CT] 4012 GAATTT AflII 2003 CTTAAG C^TTAAG 2866 CTTAAG ApoI 1436 GAATTT [GA]^AATT[CT] 4012 GAATTT Asp700I 4069 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4176 GAAGACATTC Asp718I 335 GGTACC G^GTACC 645 GGTACC AspI 473 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1318 GACGTTGTC BbrPI 1568 CACGTG CAC^GTG 3026 CACGTG BfrI 2003 CTTAAG C^TTAAG 2866 CTTAAG BsaI 759 GAGACC GGTCTC(1/5) 3144 GAGACC BsmI 1964 GAATGC GAATGC(1/-1) 4435 GAATGC BspMI 2130 ACCTGC ACCTGC(4/8) 3675 ACCTGC BsrDI 3423 CATTGC GCAATG(2/0) 4280 GCAATG BsrFI 2078 GCCGGC [GA]^CCGG[CT] 3342 ACCGGT Cfr10I 2078 GCCGGC [GA]^CCGG[CT] 3342 ACCGGT EcoT22I 3098 ATGCAT ATGCA^T 4437 ATGCAT HindIII 2492 AAGCTT A^AGCTT 4232 AAGCTT HpaI 2729 GTTAAC GTT^AAC 4491 GTTAAC KpnI 335 GGTACC GGTAC^C 645 GGTACC MfeI 4186 CAATTG C^AATTG 4945 CAATTG MunI 4186 CAATTG C^AATTG 4945 CAATTG NsiI 3098 ATGCAT ATGCA^T 4437 ATGCAT PflFI 473 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1318 GACGTTGTC PmaCI 1568 CACGTG CAC^GTG 3026 CACGTG PmlI 1568 CACGTG CAC^GTG 3026 CACGTG SalI 479 GTCGAC G^TCGAC 1351 GTCGAC SmaI 936 CCCGGG CCC^GGG 2616 CCCGGG SnaBI 114 TACGTA TAC^GTA 179 TACGTA SphI 145 GCATGC GCATG^C 3159 GCATGC Tth111I 473 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1318 GACGTTGTC XmaCI 936 CCCGGG C^CCGGG 2616 CCCGGG XmaI 936 CCCGGG C^CCGGG 2616 CCCGGG XmnI 4069 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4176 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII