##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AclI AscI AviII AvrII BciVI
BlnI BsaBI BsiEI BsiWI BspDI
BspHI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
FseI FspI KspI MamI MluI
NheI NotI NruI NspV PacI
PmeI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI SfiI SfuI
SgrAI SpeI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflIII 495 ACATGT A^C[GA][CT]GT
AgeI 3342 ACCGGT A^CCGGT
AhdI 3276 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2297 GTGCAC G^TGCAC
Aor51HI 1020 AGCGCT AGC^GCT
ApaI 1672 GGGCCC GGGCC^C
ApaLI 2297 GTGCAC G^TGCAC
AseI 1054 ATTAAT AT^TAAT
AsnI 1054 ATTAAT AT^TAAT
AspEI 3276 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 828 GGATCC G^GATCC
BbeI 1728 GGCGCC GGCGC^C
BcgI 1353 CGACCTCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2626 TGATCA T^GATCA
BglII 669 AGATCT A^GATCT
BsmBI 270 GAGACG CGTCTC(1/5)
Bsp1407I 402 TGTACA T^GTACA
BspLU11I 495 ACATGT A^CATGT
BsrBI 3259 GAGCGG CCGCTC(-3/-3)
BsrGI 402 TGTACA T^GTACA
BstAPI 4051 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 462 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4410 TTTAAA TTT^AAA
DraIII 2961 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3146 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3276 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1020 AGCGCT AGC^GCT
EcoNI 1030 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2626 TGATCA T^GATCA
KasI 1728 GGCGCC G^GCGCC
NaeI 2078 GCCGGC GCC^GGC
NarI 1728 GGCGCC GG^CGCC
NcoI 1365 CCATGG C^CATGG
NdeI 3611 CATATG CA^TATG
NgoMIV 2078 GCCGGC G^CCGGC
PaeR7I 419 CTCGAG C^TCGAG
PflMI 2046 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3342 ACCGGT A^CCGGT
PshAI 3715 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1054 ATTAAT AT^TAAT
ScaI 576 AGTACT AGT^ACT
SexAI 474 ACCTGGT A^CC[AT]GGT
SfoI 1728 GGCGCC GGC^GCC
SspBI 402 TGTACA T^GTACA
StuI 1755 AGGCCT AGG^CCT
Van91I 2046 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 419 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 272 GACGTC GACGT^C
317 GACGTC
Acc65I 335 GGTACC G^GTACC
645 GGTACC
AcsI 1436 GAATTT [GA]^AATT[CT]
4012 GAATTT
AflII 2003 CTTAAG C^TTAAG
2866 CTTAAG
ApoI 1436 GAATTT [GA]^AATT[CT]
4012 GAATTT
Asp700I 4069 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4176 GAAGACATTC
Asp718I 335 GGTACC G^GTACC
645 GGTACC
AspI 473 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1318 GACGTTGTC
BbrPI 1568 CACGTG CAC^GTG
3026 CACGTG
BfrI 2003 CTTAAG C^TTAAG
2866 CTTAAG
BsaI 759 GAGACC GGTCTC(1/5)
3144 GAGACC
BsmI 1964 GAATGC GAATGC(1/-1)
4435 GAATGC
BspMI 2130 ACCTGC ACCTGC(4/8)
3675 ACCTGC
BsrDI 3423 CATTGC GCAATG(2/0)
4280 GCAATG
BsrFI 2078 GCCGGC [GA]^CCGG[CT]
3342 ACCGGT
Cfr10I 2078 GCCGGC [GA]^CCGG[CT]
3342 ACCGGT
EcoT22I 3098 ATGCAT ATGCA^T
4437 ATGCAT
HindIII 2492 AAGCTT A^AGCTT
4232 AAGCTT
HpaI 2729 GTTAAC GTT^AAC
4491 GTTAAC
KpnI 335 GGTACC GGTAC^C
645 GGTACC
MfeI 4186 CAATTG C^AATTG
4945 CAATTG
MunI 4186 CAATTG C^AATTG
4945 CAATTG
NsiI 3098 ATGCAT ATGCA^T
4437 ATGCAT
PflFI 473 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1318 GACGTTGTC
PmaCI 1568 CACGTG CAC^GTG
3026 CACGTG
PmlI 1568 CACGTG CAC^GTG
3026 CACGTG
SalI 479 GTCGAC G^TCGAC
1351 GTCGAC
SmaI 936 CCCGGG CCC^GGG
2616 CCCGGG
SnaBI 114 TACGTA TAC^GTA
179 TACGTA
SphI 145 GCATGC GCATG^C
3159 GCATGC
Tth111I 473 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1318 GACGTTGTC
XmaCI 936 CCCGGG C^CCGGG
2616 CCCGGG
XmaI 936 CCCGGG C^CCGGG
2616 CCCGGG
XmnI 4069 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4176 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI AocI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII