##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AccIII AflII AgeI AscI
AvrII BcgI BclI BfrI BlnI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BstBI ClaI CpoI EagI
EclXI Eco52I EcoRV FbaI FseI
MfeI MluI MroI MunI NaeI
NgoMIV NotI NruI NspV PacI
PinAI PmeI PvuI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1230 GGTACC G^GTACC
AhdI 1247 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3872 GGGCCC GGGCC^C
AseI 5476 ATTAAT AT^TAAT
AsnI 5476 ATTAAT AT^TAAT
Asp718I 1230 GGTACC G^GTACC
AspEI 1247 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4342 GGATCC G^GATCC
BbeI 3524 GGCGCC GGCGC^C
BsiEI 3729 CGACCG CG[GA][CT]^CG
BsmBI 115 GAGACG CGTCTC(1/5)
Bsp1407I 5734 TGTACA T^GTACA
BsrGI 5734 TGTACA T^GTACA
Bst1107I 871 GTATAC GTA^TAC
BstZ17I 871 GTATAC GTA^TAC
DraI 5062 TTTAAA TTT^AAA
DraIII 2390 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2294 GACCGGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1247 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 3275 AAGCTT A^AGCTT
KasI 3524 GGCGCC G^GCGCC
KpnI 1230 GGTACC GGTAC^C
KspI 513 CCGCGG CCGC^GG
NarI 3524 GGCGCC GG^CGCC
NdeI 1479 CATATG CA^TATG
PaeR7I 483 CTCGAG C^TCGAG
PshAI 4288 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5476 ATTAAT AT^TAAT
SacII 513 CCGCGG CCGC^GG
SfoI 3524 GGCGCC GGC^GCC
SpeI 893 ACTAGT A^CTAGT
SspBI 5734 TGTACA T^GTACA
SspI 5371 AATATT AAT^ATT
XbaI 5569 TCTAGA T^CTAGA
XhoI 483 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 514 CGCG CG^CG
3184 CGCG
AclI 1384 AACGTT AA^CGTT
5838 AACGTT
AcyI 3524 GGCGCC G[GA]^CG[CT]C
4262 GACGCC
Aor51HI 30 AGCGCT AGC^GCT
2481 AGCGCT
Asp700I 3956 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4748 GAAATTATTC
AviII 1623 TGCGCA TGC^GCA
3561 TGCGCA
BbrPI 2533 CACGTG CAC^GTG
5586 CACGTG
BbvCI 3034 CCTCAGC CCTCAGC(-5/-2)
3581 GCTGAGG
BciVI 1316 GTATCC GTATCC(6/5)
2793 GTATCC
BglI 237 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3699 GCCTCCAGGGC
BsaBI 3307 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4354 GATCCCCATC
BsaHI 3524 GGCGCC G[GA]^CG[CT]C
4262 GACGCC
BsrBI 2829 CCGCTC CCGCTC(-3/-3)
3587 GAGCGG
BsrFI 2088 ACCGGC [GA]^CCGG[CT]
4591 ACCGGC
BssSI 938 CTCGTG CACGAG(-5/-1)
3651 CTCGTG
BstAPI 4193 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4381 GCAACGCATGC
BstUI 514 CGCG CG^CG
3184 CGCG
Cfr10I 2088 ACCGGC [GA]^CCGG[CT]
4591 ACCGGC
EarI 577 GAAGAG CTCTTC(1/4)
4645 GAAGAG
Eco47III 30 AGCGCT AGC^GCT
2481 AGCGCT
EcoRI 2457 GAATTC G^AATTC
5965 GAATTC
EcoT22I 1460 ATGCAT ATGCA^T
3568 ATGCAT
FspI 1623 TGCGCA TGC^GCA
3561 TGCGCA
Hin1I 3524 GGCGCC G[GA]^CG[CT]C
4262 GACGCC
HpaI 1387 GTTAAC GTT^AAC
3604 GTTAAC
Ksp632I 577 GAAGAG CTCTTC(1/4)
4645 GAAGAG
MamI 3307 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4354 GATCCCCATC
MvnI 514 CGCG CG^CG
3184 CGCG
NcoI 1655 CCATGG C^CATGG
5241 CCATGG
NsiI 1460 ATGCAT ATGCA^T
3568 ATGCAT
PflMI 2389 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2573 CCAGAAGTTGG
PmaCI 2533 CACGTG CAC^GTG
5586 CACGTG
PmlI 2533 CACGTG CAC^GTG
5586 CACGTG
Psp1406I 1384 AACGTT AA^CGTT
5838 AACGTT
SmlI 483 CTCGAG C^T[CT][GA]AG
3062 CTCAAG
SphI 4134 GCATGC GCATG^C
4386 GCATGC
Van91I 2389 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2573 CCAGAAGTTGG
XmnI 3956 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4748 GAAATTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NheI NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmaCI XmaI