##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AclI AflII Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AvrII BamHI BfrI BglII
BlnI BsaBI BsiEI BsiWI BspDI
BssHII BstXI ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoRV FseI HpaI KpnI MamI
MluI NaeI NgoMIV NotI PacI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 44 TCCGGA T^CCGGA
AgeI 225 ACCGGT A^CCGGT
AhdI 2434 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3525 CCTGAGG CC^T[ACGT]AGG
ApaI 810 GGGCCC GGGCC^C
AspEI 2434 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2095 TGCGCA TGC^GCA
BciVI 3027 GGATAC GTATCC(6/5)
BseAI 44 TCCGGA T^CCGGA
Bsp1407I 793 TGTACA T^GTACA
BspEI 44 TCCGGA T^CCGGA
BspHI 6043 TCATGA T^CATGA
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BsrFI 225 ACCGGT [GA]^CCGG[CT]
BsrGI 793 TGTACA T^GTACA
Bst1107I 5459 GTATAC GTA^TAC
BstEII 3362 GGTGACC G^GT[ACGT]ACC
BstPI 3362 GGTGACC G^GT[ACGT]ACC
BstZ17I 5459 GTATAC GTA^TAC
Bsu36I 3525 CCTGAGG CC^T[ACGT]AGG
Cfr10I 225 ACCGGT [GA]^CCGG[CT]
Eam1105I 2434 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3525 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3362 GGTGACC G^GT[ACGT]ACC
FspI 2095 TGCGCA TGC^GCA
KspI 170 CCGCGG CCGC^GG
MfeI 1567 CAATTG C^AATTG
MroI 44 TCCGGA T^CCGGA
MunI 1567 CAATTG C^AATTG
NheI 2676 GCTAGC G^CTAGC
PinAI 225 ACCGGT A^CCGGT
RcaI 6043 TCATGA T^CATGA
SacII 170 CCGCGG CCGC^GG
SspBI 793 TGTACA T^GTACA
XbaI 3501 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 2059 GAATTC [GA]^AATT[CT]
5213 GAATTC
ApoI 2059 GAATTC [GA]^AATT[CT]
5213 GAATTC
BbeI 88 GGCGCC GGCGC^C
165 GGCGCC
BbvCI 1055 CCTCAGC CCTCAGC(-5/-2)
4164 GCTGAGG
BcgI 29 CGAGAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3381 CGAGACAACTGC
BclI 1105 TGATCA T^GATCA
3056 TGATCA
BglI 168 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4826 GCCATTTGGGC
BsmBI 1888 GAGACG CGTCTC(1/5)
4996 GAGACG
BsrDI 2104 GCAATG GCAATG(2/0)
4691 GCAATG
BssSI 689 CACGAG CACGAG(-5/-1)
1961 CTCGTG
BstBI 446 TTCGAA TT^CGAA
3793 TTCGAA
BstYI 2737 AGATCC [GA]^GATC[CT]
4340 GGATCT
DrdI 1088 GACCTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACTATAATGTC
EcoNI 1683 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2720 CCTGACCCAGG
EcoRI 2059 GAATTC G^AATTC
5213 GAATTC
FbaI 1105 TGATCA T^GATCA
3056 TGATCA
HaeII 88 GGCGCC [GA]GCGC^[CT]
165 GGCGCC
HindIII 1489 AAGCTT A^AGCTT
5942 AAGCTT
KasI 88 GGCGCC G^GCGCC
165 GGCGCC
MflI 2737 AGATCC [GA]^GATC[CT]
4340 GGATCT
NarI 88 GGCGCC GG^CGCC
165 GGCGCC
NruI 465 TCGCGA TCG^CGA
3084 TCGCGA
NspV 446 TTCGAA TT^CGAA
3793 TTCGAA
PaeR7I 456 CTCGAG C^TCGAG
1192 CTCGAG
ScaI 562 AGTACT AGT^ACT
3823 AGTACT
SfoI 88 GGCGCC GGC^GCC
165 GGCGCC
SfuI 446 TTCGAA TT^CGAA
3793 TTCGAA
SmaI 1012 CCCGGG CCC^GGG
1420 CCCGGG
SspI 4134 AATATT AAT^ATT
5171 AATATT
XhoI 456 CTCGAG C^TCGAG
1192 CTCGAG
XhoII 2737 AGATCC [GA]^GATC[CT]
4340 GGATCT
XmaCI 1012 CCCGGG C^CCGGG
1420 CCCGGG
XmaI 1012 CCCGGG C^CCGGG
1420 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI Asp700I
AspHI AspI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XmnI