##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI Asp700I AvrII BbrPI
BglI BlnI BsaAI BsiEI BsiWI
BspDI BspHI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI HpaI
KspI MluI NaeI NgoMIV NotI
NruI NsiI NspV PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2477 GGTACC G^GTACC
AccII 10 CGCG CG^CG
AccIII 2651 TCCGGA T^CCGGA
AcyI 1869 GGCGCC G[GA]^CG[CT]C
AflII 6415 CTTAAG C^TTAAG
AhdI 3291 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2918 GTGCAC G^TGCAC
ApaI 1231 GGGCCC GGGCC^C
ApaLI 2918 GTGCAC G^TGCAC
Asp718I 2477 GGTACC G^GTACC
AspEI 3291 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2675 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5704 TGGCCA TGG^CCA
BbeI 1869 GGCGCC GGCGC^C
BbvCI 309 GCTGAGG CCTCAGC(-5/-2)
BfrI 6415 CTTAAG C^TTAAG
BsaBI 4820 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1869 GGCGCC G[GA]^CG[CT]C
BseAI 2651 TCCGGA T^CCGGA
BspEI 2651 TCCGGA T^CCGGA
BsrBI 2563 GAGCGG CCGCTC(-3/-3)
BstUI 10 CGCG CG^CG
EaeI 5704 TGGCCA [CT]^GGCC[GA]
Eam1105I 3291 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 1869 GGCGCC G[GA]^CG[CT]C
KasI 1869 GGCGCC G^GCGCC
KpnI 2477 GGTACC GGTAC^C
MamI 4820 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5704 TGGCCA TGG^CCA
MroI 2651 TCCGGA T^CCGGA
MscI 5704 TGGCCA TGG^CCA
MvnI 10 CGCG CG^CG
NarI 1869 GGCGCC GG^CGCC
NdeI 5385 CATATG CA^TATG
NheI 2608 GCTAGC G^CTAGC
PacI 6573 TTAATTAA TTAAT^TAA
PflFI 2675 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 607 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1424 ACCTGGT A^CC[AT]GGT
SfoI 1869 GGCGCC GGC^GCC
SmaI 87 CCCGGG CCC^GGG
SpeI 5534 ACTAGT A^CTAGT
SspI 5613 AATATT AAT^ATT
Tth111I 2675 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 3678 CCTCAGG CC^T[ACGT]AGG
5442 CCTAAGG
AvaI 87 CCCGGG C^[CT]CG[GA]G
1477 CTCGGG
AviII 74 TGCGCA TGC^GCA
515 TGCGCA
BclI 1028 TGATCA T^GATCA
5961 TGATCA
BlpI 2731 GCTGAGC GC^T[ACGT]AGC
3099 GCTGAGC
Bpu1102I 2731 GCTGAGC GC^T[ACGT]AGC
3099 GCTGAGC
BsmBI 3152 CGTCTC CGTCTC(1/5)
3085 GAGACG
BsoBI 87 CCCGGG C^[CT]CG[GA]G
1477 CTCGGG
BstAPI 3551 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4950 GCACACACTGC
BstEII 1493 GGTTACC G^GT[ACGT]ACC
2781 GGTGACC
BstPI 1493 GGTTACC G^GT[ACGT]ACC
2781 GGTGACC
BstXI 234 CCACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2630 CCAAGCGAATGG
Bsu36I 3678 CCTCAGG CC^T[ACGT]AGG
5442 CCTAAGG
CelII 2731 GCTGAGC GC^T[ACGT]AGC
3099 GCTGAGC
DraI 4412 TTTAAA TTT^AAA
4572 TTTAAA
Eco81I 3678 CCTCAGG CC^T[ACGT]AGG
5442 CCTAAGG
EcoO65I 1493 GGTTACC G^GT[ACGT]ACC
2781 GGTGACC
EcoRI 3475 GAATTC G^AATTC
5079 GAATTC
FbaI 1028 TGATCA T^GATCA
5961 TGATCA
FspI 74 TGCGCA TGC^GCA
515 TGCGCA
HaeII 398 GGCGCT [GA]GCGC^[CT]
1869 GGCGCC
HgaI 1952 GACGC GACGC(5/10)
612 GCGTC
HincII 3945 GTCAAC GT[CT]^[GA]AC
4070 GTCAAC
HindII 3945 GTCAAC GT[CT]^[GA]AC
4070 GTCAAC
NcoI 498 CCATGG C^CATGG
1241 CCATGG
PflMI 1175 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1860 CCAGCAGATGG
SacI 2826 GAGCTC GAGCT^C
3664 GAGCTC
ScaI 4750 AGTACT AGT^ACT
4833 AGTACT
Van91I 1175 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1860 CCAGCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BamHI BanI BanII BbsI
BbvI BcgI BciVI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII