##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AflII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AviII BbeI
BbrPI BfrI BsiWI BspDI BssHII
Bst1107I BstZ17I ClaI CpoI Eam1105I
Eco47III EcoRV FseI FspI HpaI
KasI MluI NaeI NarI NdeI
NgoMIV NotI NruI PacI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SalI SfiI SfoI SgrAI
SmaI SnaBI SpeI SspI StuI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 4050 GACGTC GACGT^C
Acc65I 4520 GGTACC G^GTACC
AccIII 5110 TCCGGA T^CCGGA
AclI 1359 AACGTT AA^CGTT
AcyI 4050 GACGTC G[GA]^CG[CT]C
Asp700I 1179 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4520 GGTACC G^GTACC
AspI 5375 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2349 GTATCC GTATCC(6/5)
BsaAI 1050 TACGTG [CT]AC^GT[GA]
BsaHI 4050 GACGTC G[GA]^CG[CT]C
BseAI 5110 TCCGGA T^CCGGA
BsmBI 3498 GAGACG CGTCTC(1/5)
BsmI 1547 GAATGC GAATGC(1/-1)
BspEI 5110 TCCGGA T^CCGGA
BsrFI 3 GCCGGT [GA]^CCGG[CT]
BstBI 733 TTCGAA TT^CGAA
BstEII 4249 GGTGACC G^GT[ACGT]ACC
BstPI 4249 GGTGACC G^GT[ACGT]ACC
BstXI 2002 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
DrdI 5538 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1 CGGCCG C^GGCCG
EclXI 1 CGGCCG C^GGCCG
Eco52I 1 CGGCCG C^GGCCG
EcoO65I 4249 GGTGACC G^GT[ACGT]ACC
EcoRI 1690 GAATTC G^AATTC
Hin1I 4050 GACGTC G[GA]^CG[CT]C
HindIII 1452 AAGCTT A^AGCTT
KpnI 4520 GGTACC GGTAC^C
KspI 106 CCGCGG CCGC^GG
MfeI 4967 CAATTG C^AATTG
MroI 5110 TCCGGA T^CCGGA
MunI 4967 CAATTG C^AATTG
NheI 4920 GCTAGC G^CTAGC
NspV 733 TTCGAA TT^CGAA
PflFI 5375 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1359 AACGTT AA^CGTT
SacII 106 CCGCGG CCGC^GG
SexAI 931 ACCTGGT A^CC[AT]GGT
SfuI 733 TTCGAA TT^CGAA
Tth111I 5375 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1179 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 107 CGCG CG^CG
4036 CGCG
Alw44I 1878 GTGCAC G^TGCAC
3618 GTGCAC
ApaI 3532 GGGCCC GGGCC^C
4231 GGGCCC
ApaLI 1878 GTGCAC G^TGCAC
3618 GTGCAC
BcgI 3110 CGAGGACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
905 GCACCGGAATCG
BglI 2707 GCCGCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2851 GCCCTGGTGGC
BglII 4382 AGATCT A^GATCT
4472 AGATCT
BsiEI 1 CGGCCG CG[GA][CT]^CG
3160 CGGTCG
Bsp1407I 688 TGTACA T^GTACA
4943 TGTACA
BspLU11I 175 ACATGT A^CATGT
1963 ACATGT
BsrDI 5314 CATTGC GCAATG(2/0)
5570 CATTGC
BsrGI 688 TGTACA T^GTACA
4943 TGTACA
BssSI 504 CACGAG CACGAG(-5/-1)
315 CTCGTG
BstAPI 1258 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3281 GCACTGAGTGC
BstUI 107 CGCG CG^CG
4036 CGCG
DraI 3732 TTTAAA TTT^AAA
4668 TTTAAA
DraIII 3127 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3282 CACTGAGTG
EcoNI 2387 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2549 CCTGGCCAAGG
EcoT22I 3339 ATGCAT ATGCA^T
5592 ATGCAT
HincII 3000 GTCAAC GT[CT]^[GA]AC
4738 GTCAAC
HindII 3000 GTCAAC GT[CT]^[GA]AC
4738 GTCAAC
MvnI 107 CGCG CG^CG
4036 CGCG
NcoI 5143 CCATGG C^CATGG
5692 CCATGG
NsiI 3339 ATGCAT ATGCA^T
5592 ATGCAT
PaeR7I 1290 CTCGAG C^TCGAG
3686 CTCGAG
PshAI 3146 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3986 GACCTGAGTC
SapI 1523 GAAGAGC GCTCTTC(1/4)
2893 GCTCTTC
SbfI 2492 CCTGCAGG CCTGCA^GG
4581 CCTGCAGG
ScaI 1844 AGTACT AGT^ACT
5427 AGTACT
Sse8387I 2492 CCTGCAGG CCTGCA^GG
4581 CCTGCAGG
SspBI 688 TGTACA T^GTACA
4943 TGTACA
XhoI 1290 CTCGAG C^TCGAG
3686 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII AvrII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII