##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AscI AviII AvrII BbeI
BbrPI BbvCI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI BspDI
BspHI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoNI EcoO65I
EcoRV FseI FspI KasI KspI
MamI MluI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SacII SexAI
SfiI SfoI SfuI SmaI SnaBI
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1766 GGTACC G^GTACC
AclI 6906 AACGTT AA^CGTT
AflII 2447 CTTAAG C^TTAAG
AgeI 5976 ACCGGT A^CCGGT
AhdI 455 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4420 GTGCAC G^TGCAC
AocI 5813 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1927 AGCGCT AGC^GCT
ApaLI 4420 GTGCAC G^TGCAC
AseI 5827 ATTAAT AT^TAAT
AsnI 5827 ATTAAT AT^TAAT
Asp718I 1766 GGTACC G^GTACC
AspEI 455 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6301 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 339 CTCGGG C^[CT]CG[GA]G
BfrI 2447 CTTAAG C^TTAAG
BglI 5426 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 339 CTCGGG C^[CT]CG[GA]G
BsrDI 4784 CATTGC GCAATG(2/0)
BstAPI 5951 GCAAACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3398 CCAGTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5813 CCTGAGG CC^T[ACGT]AGG
DrdI 4021 GACTGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 455 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1927 AGCGCT AGC^GCT
Eco81I 5813 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2052 ATGCAT ATGCA^T
HaeII 1927 AGCGCT [GA]GCGC^[CT]
KpnI 1766 GGTACC GGTAC^C
MfeI 4691 CAATTG C^AATTG
MunI 4691 CAATTG C^AATTG
NheI 1667 GCTAGC G^CTAGC
NsiI 2052 ATGCAT ATGCA^T
PflFI 6301 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5976 ACCGGT A^CCGGT
PshBI 5827 ATTAAT AT^TAAT
Psp1406I 6906 AACGTT AA^CGTT
PvuII 4650 CAGCTG CAG^CTG
SalI 778 GTCGAC G^TCGAC
SbfI 6755 CCTGCAGG CCTGCA^GG
ScaI 2972 AGTACT AGT^ACT
SpeI 2955 ACTAGT A^CTAGT
Sse8387I 6755 CCTGCAGG CCTGCA^GG
SspI 2429 AATATT AAT^ATT
StuI 2329 AGGCCT AGG^CCT
TaqI 779 TCGA T^CGA
Tth111I 6301 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 779 TCGA T^CGA
XcmI 739 CCAGCTAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 390 TCCGGA T^CCGGA
3983 TCCGGA
AcyI 5216 GGCGTC G[GA]^CG[CT]C
6617 GGCGTC
AlwNI 2042 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
5622 CAGTCCCTG
ApaI 214 GGGCCC GGGCC^C
3858 GGGCCC
BalI 405 TGGCCA TGG^CCA
3702 TGGCCA
BamHI 1946 GGATCC G^GATCC
3010 GGATCC
BcgI 1495 CGACTATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6373 CGAGCTCTCTGC
BclI 3820 TGATCA T^GATCA
4626 TGATCA
BcnI 516 CCCGG CC^[GC]GG
635 CCCGG
BlpI 1923 GCTGAGC GC^T[ACGT]AGC
3465 GCTTAGC
Bpu1102I 1923 GCTGAGC GC^T[ACGT]AGC
3465 GCTTAGC
BsaHI 5216 GGCGTC G[GA]^CG[CT]C
6617 GGCGTC
BsaI 2828 GAGACC GGTCTC(1/5)
3695 GAGACC
BseAI 390 TCCGGA T^CCGGA
3983 TCCGGA
Bsp1407I 881 TGTACA T^GTACA
6662 TGTACA
BspEI 390 TCCGGA T^CCGGA
3983 TCCGGA
BsrGI 881 TGTACA T^GTACA
6662 TGTACA
CelII 1923 GCTGAGC GC^T[ACGT]AGC
3465 GCTTAGC
DraIII 2754 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4408 CACACTGTG
EaeI 405 TGGCCA [CT]^GGCC[GA]
3702 TGGCCA
EcoRI 2117 GAATTC G^AATTC
5385 GAATTC
FbaI 3820 TGATCA T^GATCA
4626 TGATCA
Hin1I 5216 GGCGTC G[GA]^CG[CT]C
6617 GGCGTC
HpaI 2470 GTTAAC GTT^AAC
3239 GTTAAC
MluNI 405 TGGCCA TGG^CCA
3702 TGGCCA
MroI 390 TCCGGA T^CCGGA
3983 TCCGGA
MscI 405 TGGCCA TGG^CCA
3702 TGGCCA
NciI 516 CCCGG CC^[GC]GG
635 CCCGG
NdeI 2369 CATATG CA^TATG
3677 CATATG
PpuMI 101 AGGTCCC [GA]G^G[AT]CC[CT]
690 AGGACCC
SapI 232 GAAGAGC GCTCTTC(1/4)
5581 GAAGAGC
SgrAI 5975 CACCGGTG C[GA]^CCGG[CT]G
6613 CACCGGCG
SspBI 881 TGTACA T^GTACA
6662 TGTACA
XbaI 3089 TCTAGA T^CTAGA
4817 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI