##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AhdI AscI AseI
AsnI AspEI AviII AvrII BbvCI
BlnI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRV
FseI FspI HgaI HindIII KspI
MluI MroI NaeI NgoMIV NotI
NruI PacI PmeI PshBI Psp1406I
PvuI RsrII SacII SapI ScaI
SfiI SgrAI SmaI SnaBI SpeI
SspBI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 921 GACGTC GACGT^C
AccII 3134 CGCG CG^CG
AflII 1903 CTTAAG C^TTAAG
AgeI 3804 ACCGGT A^CCGGT
Alw44I 630 GTGCAC G^TGCAC
ApaLI 630 GTGCAC G^TGCAC
AspI 3068 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4207 CACGTG CAC^GTG
BfrI 1903 CTTAAG C^TTAAG
BglII 3153 AGATCT A^GATCT
BlpI 3536 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3536 GCTGAGC GC^T[ACGT]AGC
BsaBI 2063 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4458 GAGACC GGTCTC(1/5)
BsmBI 602 CGTCTC CGTCTC(1/5)
BspLU11I 2349 ACATGT A^CATGT
BsrBI 1498 CCGCTC CCGCTC(-3/-3)
BstAPI 118 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3435 TTCGAA TT^CGAA
BstEII 2791 GGTTACC G^GT[ACGT]ACC
BstPI 2791 GGTTACC G^GT[ACGT]ACC
BstUI 3134 CGCG CG^CG
CelII 3536 GCTGAGC GC^T[ACGT]AGC
DrdI 1301 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2791 GGTTACC G^GT[ACGT]ACC
EcoRI 1569 GAATTC G^AATTC
EcoT22I 681 ATGCAT ATGCA^T
HpaI 2489 GTTAAC GTT^AAC
MamI 2063 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2423 CAATTG C^AATTG
MunI 2423 CAATTG C^AATTG
MvnI 3134 CGCG CG^CG
NsiI 681 ATGCAT ATGCA^T
NspV 3435 TTCGAA TT^CGAA
PaeR7I 2058 CTCGAG C^TCGAG
PflFI 3068 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3804 ACCGGT A^CCGGT
PmaCI 4207 CACGTG CAC^GTG
PmlI 4207 CACGTG CAC^GTG
PshAI 1901 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 545 GAGCTC GAGCT^C
SexAI 1276 ACCTGGT A^CC[AT]GGT
SfuI 3435 TTCGAA TT^CGAA
SphI 2640 GCATGC GCATG^C
StuI 1637 AGGCCT AGG^CCT
Tth111I 3068 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2058 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 876 GTCGAC GT^[AC][GT]AC
918 GTCGAC
AflIII 2349 ACATGT A^C[GA][CT]GT
4206 ACACGT
Aor51HI 591 AGCGCT AGC^GCT
4616 AGCGCT
Asp700I 157 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1320 GAACAGCTTC
BamHI 1051 GGATCC G^GATCC
4498 GGATCC
BbeI 1860 GGCGCC GGCGC^C
3501 GGCGCC
BcgI 61 CGAGTTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2023 GCACAGCAATCG
BglI 485 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4651 GCCACAGGGGC
BsaAI 3092 CACGTA [CT]AC^GT[GA]
4207 CACGTG
BsaWI 2408 TCCGGT [AT]^CCGG[AT]
3804 ACCGGT
BsrFI 27 ACCGGC [GA]^CCGG[CT]
3804 ACCGGT
Cfr10I 27 ACCGGC [GA]^CCGG[CT]
3804 ACCGGT
DraI 5023 TTTAAA TTT^AAA
5039 TTTAAA
Eco47III 591 AGCGCT AGC^GCT
4616 AGCGCT
FauI 884 GCGGG CCCGC(4/6)
2257 GCGGG
KasI 1860 GGCGCC G^GCGCC
3501 GGCGCC
NarI 1860 GGCGCC GG^CGCC
3501 GGCGCC
NdeI 1169 CATATG CA^TATG
4555 CATATG
NheI 2917 GCTAGC G^CTAGC
3700 GCTAGC
PpuMI 1212 AGGACCC [GA]G^G[AT]CC[CT]
1299 GGGACCT
SalI 876 GTCGAC G^TCGAC
918 GTCGAC
SfoI 1860 GGCGCC GGC^GCC
3501 GGCGCC
SspI 2714 AATATT AAT^ATT
5034 AATATT
XcmI 1118 CCACCTTTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3348 CCAACCTGCAGCTGG
XmnI 157 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1320 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII