################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AccIII AclI AhdI AscI AseI AsnI AspEI AviII AvrII BbvCI BlnI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI BssHII BssSI Bst1107I BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV FseI FspI HgaI HindIII KspI MluI MroI NaeI NgoMIV NotI NruI PacI PmeI PshBI Psp1406I PvuI RsrII SacII SapI ScaI SfiI SgrAI SmaI SnaBI SpeI SspBI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 921 GACGTC GACGT^C AccII 3134 CGCG CG^CG AflII 1903 CTTAAG C^TTAAG AgeI 3804 ACCGGT A^CCGGT Alw44I 630 GTGCAC G^TGCAC ApaLI 630 GTGCAC G^TGCAC AspI 3068 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 4207 CACGTG CAC^GTG BfrI 1903 CTTAAG C^TTAAG BglII 3153 AGATCT A^GATCT BlpI 3536 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3536 GCTGAGC GC^T[ACGT]AGC BsaBI 2063 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4458 GAGACC GGTCTC(1/5) BsmBI 602 CGTCTC CGTCTC(1/5) BspLU11I 2349 ACATGT A^CATGT BsrBI 1498 CCGCTC CCGCTC(-3/-3) BstAPI 118 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3435 TTCGAA TT^CGAA BstEII 2791 GGTTACC G^GT[ACGT]ACC BstPI 2791 GGTTACC G^GT[ACGT]ACC BstUI 3134 CGCG CG^CG CelII 3536 GCTGAGC GC^T[ACGT]AGC DrdI 1301 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2791 GGTTACC G^GT[ACGT]ACC EcoRI 1569 GAATTC G^AATTC EcoT22I 681 ATGCAT ATGCA^T HpaI 2489 GTTAAC GTT^AAC MamI 2063 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2423 CAATTG C^AATTG MunI 2423 CAATTG C^AATTG MvnI 3134 CGCG CG^CG NsiI 681 ATGCAT ATGCA^T NspV 3435 TTCGAA TT^CGAA PaeR7I 2058 CTCGAG C^TCGAG PflFI 3068 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3804 ACCGGT A^CCGGT PmaCI 4207 CACGTG CAC^GTG PmlI 4207 CACGTG CAC^GTG PshAI 1901 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 545 GAGCTC GAGCT^C SexAI 1276 ACCTGGT A^CC[AT]GGT SfuI 3435 TTCGAA TT^CGAA SphI 2640 GCATGC GCATG^C StuI 1637 AGGCCT AGG^CCT Tth111I 3068 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2058 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 876 GTCGAC GT^[AC][GT]AC 918 GTCGAC AflIII 2349 ACATGT A^C[GA][CT]GT 4206 ACACGT Aor51HI 591 AGCGCT AGC^GCT 4616 AGCGCT Asp700I 157 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1320 GAACAGCTTC BamHI 1051 GGATCC G^GATCC 4498 GGATCC BbeI 1860 GGCGCC GGCGC^C 3501 GGCGCC BcgI 61 CGAGTTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2023 GCACAGCAATCG BglI 485 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4651 GCCACAGGGGC BsaAI 3092 CACGTA [CT]AC^GT[GA] 4207 CACGTG BsaWI 2408 TCCGGT [AT]^CCGG[AT] 3804 ACCGGT BsrFI 27 ACCGGC [GA]^CCGG[CT] 3804 ACCGGT Cfr10I 27 ACCGGC [GA]^CCGG[CT] 3804 ACCGGT DraI 5023 TTTAAA TTT^AAA 5039 TTTAAA Eco47III 591 AGCGCT AGC^GCT 4616 AGCGCT FauI 884 GCGGG CCCGC(4/6) 2257 GCGGG KasI 1860 GGCGCC G^GCGCC 3501 GGCGCC NarI 1860 GGCGCC GG^CGCC 3501 GGCGCC NdeI 1169 CATATG CA^TATG 4555 CATATG NheI 2917 GCTAGC G^CTAGC 3700 GCTAGC PpuMI 1212 AGGACCC [GA]G^G[AT]CC[CT] 1299 GGGACCT SalI 876 GTCGAC G^TCGAC 918 GTCGAC SfoI 1860 GGCGCC GGC^GCC 3501 GGCGCC SspI 2714 AATATT AAT^ATT 5034 AATATT XcmI 1118 CCACCTTTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3348 CCAACCTGCAGCTGG XmnI 157 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1320 GAACAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### Acc65I AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII