##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvrII BcgI
BfrI BlnI BsaBI BsaWI BseAI
BsiEI BspDI BspEI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 5024 GTAGAC GT^[AC][GT]AC
AcyI 815 GGCGCC G[GA]^CG[CT]C
BbeI 815 GGCGCC GGCGC^C
BbrPI 2384 CACGTG CAC^GTG
BbvCI 2762 GCTGAGG CCTCAGC(-5/-2)
BsaAI 2384 CACGTG [CT]AC^GT[GA]
BsaHI 815 GGCGCC G[GA]^CG[CT]C
BsiWI 335 CGTACG C^GTACG
BsmI 1735 GAATGC GAATGC(1/-1)
BsrDI 3375 GCAATG GCAATG(2/0)
BssHII 3331 GCGCGC G^CGCGC
BssSI 2931 CACGAG CACGAG(-5/-1)
BstEII 4513 GGTCACC G^GT[ACGT]ACC
BstPI 4513 GGTCACC G^GT[ACGT]ACC
EcoO65I 4513 GGTCACC G^GT[ACGT]ACC
EcoT22I 1847 ATGCAT ATGCA^T
Hin1I 815 GGCGCC G[GA]^CG[CT]C
HindIII 1964 AAGCTT A^AGCTT
HpaI 2830 GTTAAC GTT^AAC
KasI 815 GGCGCC G^GCGCC
NaeI 404 GCCGGC GCC^GGC
NarI 815 GGCGCC GG^CGCC
NgoMIV 404 GCCGGC G^CCGGC
NsiI 1847 ATGCAT ATGCA^T
PacI 5084 TTAATTAA TTAAT^TAA
PmaCI 2384 CACGTG CAC^GTG
PmlI 2384 CACGTG CAC^GTG
SbfI 3175 CCTGCAGG CCTGCA^GG
ScaI 364 AGTACT AGT^ACT
SfoI 815 GGCGCC GGC^GCC
SphI 979 GCATGC GCATG^C
Sse8387I 3175 CCTGCAGG CCTGCA^GG
XbaI 4321 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 240 GTGCAC G^TGCAC
1673 GTGCAC
AlwNI 1262 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2501 CAGAGCCTG
AocI 2072 CCTCAGG CC^T[ACGT]AGG
2756 CCTGAGG
ApaLI 240 GTGCAC G^TGCAC
1673 GTGCAC
Asp700I 1961 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2080 GAAGAACTTC
AviII 2442 TGCGCA TGC^GCA
4877 TGCGCA
BciVI 1545 GTATCC GTATCC(6/5)
3735 GTATCC
BclI 1389 TGATCA T^GATCA
3348 TGATCA
BglII 3627 AGATCT A^GATCT
5050 AGATCT
BsmBI 1749 CGTCTC CGTCTC(1/5)
258 GAGACG
BspHI 2920 TCATGA T^CATGA
3345 TCATGA
BspLU11I 4851 ACATGT A^CATGT
4872 ACATGT
Bsu36I 2072 CCTCAGG CC^T[ACGT]AGG
2756 CCTGAGG
Eco81I 2072 CCTCAGG CC^T[ACGT]AGG
2756 CCTGAGG
EcoRI 2597 GAATTC G^AATTC
5013 GAATTC
FbaI 1389 TGATCA T^GATCA
3348 TGATCA
FspI 2442 TGCGCA TGC^GCA
4877 TGCGCA
HgaI 260 GACGC GACGC(5/10)
686 GACGC
RcaI 2920 TCATGA T^CATGA
3345 TCATGA
StuI 987 AGGCCT AGG^CCT
2866 AGGCCT
XmnI 1961 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2080 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI Asp718I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII