################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AclI AscI BbeI BbrPI BcgI BglII BsaAI BsiEI BsmI BspDI BssHII BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRV FseI KasI MluI NarI NdeI NotI NspV PacI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SapI SfiI SfoI SfuI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccIII 297 TCCGGA T^CCGGA AflII 4444 CTTAAG C^TTAAG AgeI 108 ACCGGT A^CCGGT AhdI 3244 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 130 GTGCAC G^TGCAC Aor51HI 49 AGCGCT AGC^GCT ApaLI 130 GTGCAC G^TGCAC AseI 5399 ATTAAT AT^TAAT AsnI 5399 ATTAAT AT^TAAT Asp700I 4023 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3244 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1541 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3983 TGCGCA TGC^GCA BalI 3482 TGGCCA TGG^CCA BamHI 257 GGATCC G^GATCC BclI 3867 TGATCA T^GATCA BfrI 4444 CTTAAG C^TTAAG BglI 749 GCCAGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1603 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 297 TCCGGA T^CCGGA BsiWI 3327 CGTACG C^GTACG BspEI 297 TCCGGA T^CCGGA BspHI 924 TCATGA T^CATGA BsrDI 5032 GCAATG GCAATG(2/0) BstAPI 30 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 2882 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3244 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 49 AGCGCT AGC^GCT EcoNI 2338 CCTTAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 3867 TGATCA T^GATCA FspI 3983 TGCGCA TGC^GCA HaeII 49 AGCGCT [GA]GCGC^[CT] HpaI 3417 GTTAAC GTT^AAC KspI 116 CCGCGG CCGC^GG MamI 1603 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3534 CAATTG C^AATTG MluNI 3482 TGGCCA TGG^CCA MroI 297 TCCGGA T^CCGGA MscI 3482 TGGCCA TGG^CCA MunI 3534 CAATTG C^AATTG NaeI 2125 GCCGGC GCC^GGC NcoI 2934 CCATGG C^CATGG NgoMIV 2125 GCCGGC G^CCGGC NheI 3924 GCTAGC G^CTAGC NruI 735 TCGCGA TCG^CGA PaeR7I 3351 CTCGAG C^TCGAG PflFI 1541 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 108 ACCGGT A^CCGGT PshBI 5399 ATTAAT AT^TAAT RcaI 924 TCATGA T^CATGA SacII 116 CCGCGG CCGC^GG ScaI 2859 AGTACT AGT^ACT SexAI 1187 ACCTGGT A^CC[AT]GGT SgrAI 107 CACCGGTG C[GA]^CCGG[CT]G SphI 4264 GCATGC GCATG^C Tth111I 1541 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1686 CCAGGAATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3351 CTCGAG C^TCGAG XmnI 4023 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AatII 739 GACGTC GACGT^C 2240 GACGTC Acc65I 1160 GGTACC G^GTACC 1778 GGTACC AocI 389 CCTCAGG CC^T[ACGT]AGG 3028 CCTCAGG Asp718I 1160 GGTACC G^GTACC 1778 GGTACC AvrII 3929 CCTAGG C^CTAGG 4050 CCTAGG BlnI 3929 CCTAGG C^CTAGG 4050 CCTAGG BlpI 1560 GCTCAGC GC^T[ACGT]AGC 1674 GCTCAGC Bpu1102I 1560 GCTCAGC GC^T[ACGT]AGC 1674 GCTCAGC BsaI 1100 GAGACC GGTCTC(1/5) 4101 GAGACC BsaWI 108 ACCGGT [AT]^CCGG[AT] 297 TCCGGA BsgI 2455 CTGCAC GTGCAG(16/14) 3964 CTGCAC BsmBI 191 CGTCTC CGTCTC(1/5) 698 CGTCTC BspLU11I 813 ACATGT A^CATGT 4659 ACATGT BsrBI 2393 CCGCTC CCGCTC(-3/-3) 3012 CCGCTC BstEII 1400 GGTGACC G^GT[ACGT]ACC 2333 GGTCACC BstPI 1400 GGTGACC G^GT[ACGT]ACC 2333 GGTCACC BstYI 257 GGATCC [GA]^GATC[CT] 2077 GGATCT Bsu36I 389 CCTCAGG CC^T[ACGT]AGG 3028 CCTCAGG CelII 1560 GCTCAGC GC^T[ACGT]AGC 1674 GCTCAGC Eco81I 389 CCTCAGG CC^T[ACGT]AGG 3028 CCTCAGG EcoO65I 1400 GGTGACC G^GT[ACGT]ACC 2333 GGTCACC EcoT22I 410 ATGCAT ATGCA^T 5227 ATGCAT HincII 1142 GTTGAC GT[CT]^[GA]AC 3417 GTTAAC HindII 1142 GTTGAC GT[CT]^[GA]AC 3417 GTTAAC KpnI 1160 GGTACC GGTAC^C 1778 GGTACC MflI 257 GGATCC [GA]^GATC[CT] 2077 GGATCT NsiI 410 ATGCAT ATGCA^T 5227 ATGCAT PpuMI 2538 AGGTCCT [GA]G^G[AT]CC[CT] 4286 GGGACCT SacI 668 GAGCTC GAGCT^C 1847 GAGCTC SbfI 1528 CCTGCAGG CCTGCA^GG 1822 CCTGCAGG SmaI 762 CCCGGG CCC^GGG 1748 CCCGGG Sse8387I 1528 CCTGCAGG CCTGCA^GG 1822 CCTGCAGG XhoII 257 GGATCC [GA]^GATC[CT] 2077 GGATCT XmaCI 762 CCCGGG C^CCGGG 1748 CCCGGG XmaI 762 CCCGGG C^CCGGG 1748 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I