##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AclI        AscI        BbeI        BbrPI       BcgI        
BglII       BsaAI       BsiEI       BsmI        BspDI       
BssHII      BssSI       Bst1107I    BstBI       BstXI       
BstZ17I     ClaI        CpoI        EagI        EclXI       
Eco52I      EcoRV       FseI        KasI        MluI        
NarI        NdeI        NotI        NspV        PacI        
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SalI        SapI        SfiI        
SfoI        SfuI        SnaBI       SpeI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccIII        297  TCCGGA               T^CCGGA
AflII        4444  CTTAAG               C^TTAAG
AgeI          108  ACCGGT               A^CCGGT
AhdI         3244  GACGTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        130  GTGCAC               G^TGCAC
Aor51HI        49  AGCGCT               AGC^GCT
ApaLI         130  GTGCAC               G^TGCAC
AseI         5399  ATTAAT               AT^TAAT
AsnI         5399  ATTAAT               AT^TAAT
Asp700I      4023  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3244  GACGTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1541  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        3983  TGCGCA               TGC^GCA
BalI         3482  TGGCCA               TGG^CCA
BamHI         257  GGATCC               G^GATCC
BclI         3867  TGATCA               T^GATCA
BfrI         4444  CTTAAG               C^TTAAG
BglI          749  GCCAGCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1603  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         297  TCCGGA               T^CCGGA
BsiWI        3327  CGTACG               C^GTACG
BspEI         297  TCCGGA               T^CCGGA
BspHI         924  TCATGA               T^CATGA
BsrDI        5032  GCAATG               GCAATG(2/0)
BstAPI         30  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII       2882  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3244  GACGTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       49  AGCGCT               AGC^GCT
EcoNI        2338  CCTTAGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         3867  TGATCA               T^GATCA
FspI         3983  TGCGCA               TGC^GCA
HaeII          49  AGCGCT               [GA]GCGC^[CT]
HpaI         3417  GTTAAC               GTT^AAC
KspI          116  CCGCGG               CCGC^GG
MamI         1603  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3534  CAATTG               C^AATTG
MluNI        3482  TGGCCA               TGG^CCA
MroI          297  TCCGGA               T^CCGGA
MscI         3482  TGGCCA               TGG^CCA
MunI         3534  CAATTG               C^AATTG
NaeI         2125  GCCGGC               GCC^GGC
NcoI         2934  CCATGG               C^CATGG
NgoMIV       2125  GCCGGC               G^CCGGC
NheI         3924  GCTAGC               G^CTAGC
NruI          735  TCGCGA               TCG^CGA
PaeR7I       3351  CTCGAG               C^TCGAG
PflFI        1541  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         108  ACCGGT               A^CCGGT
PshBI        5399  ATTAAT               AT^TAAT
RcaI          924  TCATGA               T^CATGA
SacII         116  CCGCGG               CCGC^GG
ScaI         2859  AGTACT               AGT^ACT
SexAI        1187  ACCTGGT              A^CC[AT]GGT
SgrAI         107  CACCGGTG             C[GA]^CCGG[CT]G
SphI         4264  GCATGC               GCATG^C
Tth111I      1541  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1686  CCAGGAATTCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         3351  CTCGAG               C^TCGAG
XmnI         4023  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AatII         739  GACGTC               GACGT^C
             2240  GACGTC              
Acc65I       1160  GGTACC               G^GTACC
             1778  GGTACC              
AocI          389  CCTCAGG              CC^T[ACGT]AGG
             3028  CCTCAGG             
Asp718I      1160  GGTACC               G^GTACC
             1778  GGTACC              
AvrII        3929  CCTAGG               C^CTAGG
             4050  CCTAGG              
BlnI         3929  CCTAGG               C^CTAGG
             4050  CCTAGG              
BlpI         1560  GCTCAGC              GC^T[ACGT]AGC
             1674  GCTCAGC             
Bpu1102I     1560  GCTCAGC              GC^T[ACGT]AGC
             1674  GCTCAGC             
BsaI         1100  GAGACC               GGTCTC(1/5)
             4101  GAGACC              
BsaWI         108  ACCGGT               [AT]^CCGG[AT]
              297  TCCGGA              
BsgI         2455  CTGCAC               GTGCAG(16/14)
             3964  CTGCAC              
BsmBI         191  CGTCTC               CGTCTC(1/5)
              698  CGTCTC              
BspLU11I      813  ACATGT               A^CATGT
             4659  ACATGT              
BsrBI        2393  CCGCTC               CCGCTC(-3/-3)
             3012  CCGCTC              
BstEII       1400  GGTGACC              G^GT[ACGT]ACC
             2333  GGTCACC             
BstPI        1400  GGTGACC              G^GT[ACGT]ACC
             2333  GGTCACC             
BstYI         257  GGATCC               [GA]^GATC[CT]
             2077  GGATCT              
Bsu36I        389  CCTCAGG              CC^T[ACGT]AGG
             3028  CCTCAGG             
CelII        1560  GCTCAGC              GC^T[ACGT]AGC
             1674  GCTCAGC             
Eco81I        389  CCTCAGG              CC^T[ACGT]AGG
             3028  CCTCAGG             
EcoO65I      1400  GGTGACC              G^GT[ACGT]ACC
             2333  GGTCACC             
EcoT22I       410  ATGCAT               ATGCA^T
             5227  ATGCAT              
HincII       1142  GTTGAC               GT[CT]^[GA]AC
             3417  GTTAAC              
HindII       1142  GTTGAC               GT[CT]^[GA]AC
             3417  GTTAAC              
KpnI         1160  GGTACC               GGTAC^C
             1778  GGTACC              
MflI          257  GGATCC               [GA]^GATC[CT]
             2077  GGATCT              
NsiI          410  ATGCAT               ATGCA^T
             5227  ATGCAT              
PpuMI        2538  AGGTCCT              [GA]G^G[AT]CC[CT]
             4286  GGGACCT             
SacI          668  GAGCTC               GAGCT^C
             1847  GAGCTC              
SbfI         1528  CCTGCAGG             CCTGCA^GG
             1822  CCTGCAGG            
SmaI          762  CCCGGG               CCC^GGG
             1748  CCCGGG              
Sse8387I     1528  CCTGCAGG             CCTGCA^GG
             1822  CCTGCAGG            
XhoII         257  GGATCC               [GA]^GATC[CT]
             2077  GGATCT              
XmaCI         762  CCCGGG               C^CCGGG
             1748  CCCGGG              
XmaI          762  CCCGGG               C^CCGGG
             1748  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApaI        ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvCI       BbvI        
BciVI       BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspMI       BsrFI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DrdI        DsaI        
EaeI        EarI        EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I