##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AclI AscI BbeI BbrPI BcgI
BglII BsaAI BsiEI BsmI BspDI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoRV FseI KasI MluI
NarI NdeI NotI NspV PacI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SapI SfiI
SfoI SfuI SnaBI SpeI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 297 TCCGGA T^CCGGA
AflII 4444 CTTAAG C^TTAAG
AgeI 108 ACCGGT A^CCGGT
AhdI 3244 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 130 GTGCAC G^TGCAC
Aor51HI 49 AGCGCT AGC^GCT
ApaLI 130 GTGCAC G^TGCAC
AseI 5399 ATTAAT AT^TAAT
AsnI 5399 ATTAAT AT^TAAT
Asp700I 4023 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3244 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1541 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3983 TGCGCA TGC^GCA
BalI 3482 TGGCCA TGG^CCA
BamHI 257 GGATCC G^GATCC
BclI 3867 TGATCA T^GATCA
BfrI 4444 CTTAAG C^TTAAG
BglI 749 GCCAGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1603 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 297 TCCGGA T^CCGGA
BsiWI 3327 CGTACG C^GTACG
BspEI 297 TCCGGA T^CCGGA
BspHI 924 TCATGA T^CATGA
BsrDI 5032 GCAATG GCAATG(2/0)
BstAPI 30 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2882 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3244 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 49 AGCGCT AGC^GCT
EcoNI 2338 CCTTAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3867 TGATCA T^GATCA
FspI 3983 TGCGCA TGC^GCA
HaeII 49 AGCGCT [GA]GCGC^[CT]
HpaI 3417 GTTAAC GTT^AAC
KspI 116 CCGCGG CCGC^GG
MamI 1603 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3534 CAATTG C^AATTG
MluNI 3482 TGGCCA TGG^CCA
MroI 297 TCCGGA T^CCGGA
MscI 3482 TGGCCA TGG^CCA
MunI 3534 CAATTG C^AATTG
NaeI 2125 GCCGGC GCC^GGC
NcoI 2934 CCATGG C^CATGG
NgoMIV 2125 GCCGGC G^CCGGC
NheI 3924 GCTAGC G^CTAGC
NruI 735 TCGCGA TCG^CGA
PaeR7I 3351 CTCGAG C^TCGAG
PflFI 1541 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 108 ACCGGT A^CCGGT
PshBI 5399 ATTAAT AT^TAAT
RcaI 924 TCATGA T^CATGA
SacII 116 CCGCGG CCGC^GG
ScaI 2859 AGTACT AGT^ACT
SexAI 1187 ACCTGGT A^CC[AT]GGT
SgrAI 107 CACCGGTG C[GA]^CCGG[CT]G
SphI 4264 GCATGC GCATG^C
Tth111I 1541 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1686 CCAGGAATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3351 CTCGAG C^TCGAG
XmnI 4023 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 739 GACGTC GACGT^C
2240 GACGTC
Acc65I 1160 GGTACC G^GTACC
1778 GGTACC
AocI 389 CCTCAGG CC^T[ACGT]AGG
3028 CCTCAGG
Asp718I 1160 GGTACC G^GTACC
1778 GGTACC
AvrII 3929 CCTAGG C^CTAGG
4050 CCTAGG
BlnI 3929 CCTAGG C^CTAGG
4050 CCTAGG
BlpI 1560 GCTCAGC GC^T[ACGT]AGC
1674 GCTCAGC
Bpu1102I 1560 GCTCAGC GC^T[ACGT]AGC
1674 GCTCAGC
BsaI 1100 GAGACC GGTCTC(1/5)
4101 GAGACC
BsaWI 108 ACCGGT [AT]^CCGG[AT]
297 TCCGGA
BsgI 2455 CTGCAC GTGCAG(16/14)
3964 CTGCAC
BsmBI 191 CGTCTC CGTCTC(1/5)
698 CGTCTC
BspLU11I 813 ACATGT A^CATGT
4659 ACATGT
BsrBI 2393 CCGCTC CCGCTC(-3/-3)
3012 CCGCTC
BstEII 1400 GGTGACC G^GT[ACGT]ACC
2333 GGTCACC
BstPI 1400 GGTGACC G^GT[ACGT]ACC
2333 GGTCACC
BstYI 257 GGATCC [GA]^GATC[CT]
2077 GGATCT
Bsu36I 389 CCTCAGG CC^T[ACGT]AGG
3028 CCTCAGG
CelII 1560 GCTCAGC GC^T[ACGT]AGC
1674 GCTCAGC
Eco81I 389 CCTCAGG CC^T[ACGT]AGG
3028 CCTCAGG
EcoO65I 1400 GGTGACC G^GT[ACGT]ACC
2333 GGTCACC
EcoT22I 410 ATGCAT ATGCA^T
5227 ATGCAT
HincII 1142 GTTGAC GT[CT]^[GA]AC
3417 GTTAAC
HindII 1142 GTTGAC GT[CT]^[GA]AC
3417 GTTAAC
KpnI 1160 GGTACC GGTAC^C
1778 GGTACC
MflI 257 GGATCC [GA]^GATC[CT]
2077 GGATCT
NsiI 410 ATGCAT ATGCA^T
5227 ATGCAT
PpuMI 2538 AGGTCCT [GA]G^G[AT]CC[CT]
4286 GGGACCT
SacI 668 GAGCTC GAGCT^C
1847 GAGCTC
SbfI 1528 CCTGCAGG CCTGCA^GG
1822 CCTGCAGG
SmaI 762 CCCGGG CCC^GGG
1748 CCCGGG
Sse8387I 1528 CCTGCAGG CCTGCA^GG
1822 CCTGCAGG
XhoII 257 GGATCC [GA]^GATC[CT]
2077 GGATCT
XmaCI 762 CCCGGG C^CCGGG
1748 CCCGGG
XmaI 762 CCCGGG C^CCGGG
1748 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I