##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspI AviII AvrII BalI
BbeI BbrPI BcgI BlnI BsaHI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII Bst1107I
BstZ17I Bsu36I ClaI CpoI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MluI MluNI MroI MscI
NarI NotI NruI PacI PaeR7I
PflFI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SfiI
SfoI SgrAI SnaBI SpeI SspBI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 4343 GTAGAC GT^[AC][GT]AC
AflII 5341 CTTAAG C^TTAAG
AflIII 28 ACATGT A^C[GA][CT]GT
AgeI 491 ACCGGT A^CCGGT
AhdI 3914 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3914 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1505 GGATCC G^GATCC
BanI 1512 GGTGCC G^G[CT][GA]CC
BfrI 5341 CTTAAG C^TTAAG
BglI 5420 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1209 GAGACC GGTCTC(1/5)
BsaWI 491 ACCGGT [AT]^CCGG[AT]
BsmBI 1062 GAGACG CGTCTC(1/5)
BspHI 511 TCATGA T^CATGA
BspLU11I 28 ACATGT A^CATGT
BstAPI 5030 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 5276 TTTAAA TTT^AAA
DraIII 3646 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4351 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3914 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3303 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3324 GAATTC G^AATTC
HindIII 3814 AAGCTT A^AGCTT
NaeI 4074 GCCGGC GCC^GGC
NcoI 4690 CCATGG C^CATGG
NdeI 2015 CATATG CA^TATG
NgoMIV 4074 GCCGGC G^CCGGC
PflMI 5364 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 491 ACCGGT A^CCGGT
PshAI 4288 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 511 TCATGA T^CATGA
SbfI 161 CCTGCAGG CCTGCA^GG
SexAI 2801 ACCTGGT A^CC[AT]GGT
SmaI 4750 CCCGGG CCC^GGG
SphI 5386 GCATGC GCATG^C
Sse8387I 161 CCTGCAGG CCTGCA^GG
SspI 1857 AATATT AAT^ATT
Van91I 5364 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4750 CCCGGG C^CCGGG
XmaI 4750 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 787 CGCG CG^CG
6111 CGCG
ApaI 50 GGGCCC GGGCC^C
4091 GGGCCC
AvaI 4750 CCCGGG C^[CT]CG[GA]G
5424 CTCGGG
BbvCI 5889 CCTCAGC CCTCAGC(-5/-2)
1674 GCTGAGG
BglII 3398 AGATCT A^GATCT
4601 AGATCT
BsaAI 3108 CACGTA [CT]AC^GT[GA]
6192 CACGTA
BsmI 5019 GAATGC GAATGC(1/-1)
938 GCATTC
BsoBI 4750 CCCGGG C^[CT]CG[GA]G
5424 CTCGGG
BstEII 840 GGTAACC G^GT[ACGT]ACC
5829 GGTGACC
BstPI 840 GGTAACC G^GT[ACGT]ACC
5829 GGTGACC
BstUI 787 CGCG CG^CG
6111 CGCG
BstXI 1660 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5818 CCATGAGCTTGG
EcoO65I 840 GGTAACC G^GT[ACGT]ACC
5829 GGTGACC
FauI 4401 CCCGC CCCGC(4/6)
1040 GCGGG
MspA1I 1029 CAGCTG C[AC]G^C[GT]G
4416 CAGCTG
MvnI 787 CGCG CG^CG
6111 CGCG
NheI 4722 GCTAGC G^CTAGC
4961 GCTAGC
PpuMI 1401 GGGACCT [GA]G^G[AT]CC[CT]
4839 AGGACCT
PvuII 1029 CAGCTG CAG^CTG
4416 CAGCTG
ScaI 1239 AGTACT AGT^ACT
5440 AGTACT
XcmI 54 CCAGTGCAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
977 CCATGCAGGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BciVI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstBI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MnlI
MseI MslI MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI NspV PleI PshBI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfuI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI