################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspI AviII AvrII BalI BbeI BbrPI BcgI BlnI BsaHI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII Bst1107I BstZ17I Bsu36I ClaI CpoI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoRV FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MluI MluNI MroI MscI NarI NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SgrAI SnaBI SpeI SspBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 4343 GTAGAC GT^[AC][GT]AC AflII 5341 CTTAAG C^TTAAG AflIII 28 ACATGT A^C[GA][CT]GT AgeI 491 ACCGGT A^CCGGT AhdI 3914 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 3914 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1505 GGATCC G^GATCC BanI 1512 GGTGCC G^G[CT][GA]CC BfrI 5341 CTTAAG C^TTAAG BglI 5420 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 1209 GAGACC GGTCTC(1/5) BsaWI 491 ACCGGT [AT]^CCGG[AT] BsmBI 1062 GAGACG CGTCTC(1/5) BspHI 511 TCATGA T^CATGA BspLU11I 28 ACATGT A^CATGT BstAPI 5030 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 5276 TTTAAA TTT^AAA DraIII 3646 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4351 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3914 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3303 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3324 GAATTC G^AATTC HindIII 3814 AAGCTT A^AGCTT NaeI 4074 GCCGGC GCC^GGC NcoI 4690 CCATGG C^CATGG NdeI 2015 CATATG CA^TATG NgoMIV 4074 GCCGGC G^CCGGC PflMI 5364 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 491 ACCGGT A^CCGGT PshAI 4288 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 511 TCATGA T^CATGA SbfI 161 CCTGCAGG CCTGCA^GG SexAI 2801 ACCTGGT A^CC[AT]GGT SmaI 4750 CCCGGG CCC^GGG SphI 5386 GCATGC GCATG^C Sse8387I 161 CCTGCAGG CCTGCA^GG SspI 1857 AATATT AAT^ATT Van91I 5364 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 4750 CCCGGG C^CCGGG XmaI 4750 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 787 CGCG CG^CG 6111 CGCG ApaI 50 GGGCCC GGGCC^C 4091 GGGCCC AvaI 4750 CCCGGG C^[CT]CG[GA]G 5424 CTCGGG BbvCI 5889 CCTCAGC CCTCAGC(-5/-2) 1674 GCTGAGG BglII 3398 AGATCT A^GATCT 4601 AGATCT BsaAI 3108 CACGTA [CT]AC^GT[GA] 6192 CACGTA BsmI 5019 GAATGC GAATGC(1/-1) 938 GCATTC BsoBI 4750 CCCGGG C^[CT]CG[GA]G 5424 CTCGGG BstEII 840 GGTAACC G^GT[ACGT]ACC 5829 GGTGACC BstPI 840 GGTAACC G^GT[ACGT]ACC 5829 GGTGACC BstUI 787 CGCG CG^CG 6111 CGCG BstXI 1660 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5818 CCATGAGCTTGG EcoO65I 840 GGTAACC G^GT[ACGT]ACC 5829 GGTGACC FauI 4401 CCCGC CCCGC(4/6) 1040 GCGGG MspA1I 1029 CAGCTG C[AC]G^C[GT]G 4416 CAGCTG MvnI 787 CGCG CG^CG 6111 CGCG NheI 4722 GCTAGC G^CTAGC 4961 GCTAGC PpuMI 1401 GGGACCT [GA]G^G[AT]CC[CT] 4839 AGGACCT PvuII 1029 CAGCTG CAG^CTG 4416 CAGCTG ScaI 1239 AGTACT AGT^ACT 5440 AGTACT XcmI 54 CCAGTGCAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 977 CCATGCAGGCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BanII BbsI BbvI BciVI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstBI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI NspV PleI PshBI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI