##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccII AccIII AclI Aor51HI
AscI Asp718I AviII BamHI BbeI
BcgI BglII BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BstAPI BstUI ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV FspI
HgaI KasI KpnI KspI MamI
MluI MroI MvnI NarI NheI
NotI NruI PacI PmeI Psp1406I
RsrII SacII SalI SbfI ScaI
SfoI SgrAI SpeI Sse8387I SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 656 GACGTC GACGT^C
AcyI 656 GACGTC G[GA]^CG[CT]C
AgeI 352 ACCGGT A^CCGGT
AhdI 3033 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 798 ATTAAT AT^TAAT
AsnI 798 ATTAAT AT^TAAT
AspEI 3033 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3701 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 1294 GTATCC GTATCC(6/5)
BsaHI 656 GACGTC G[GA]^CG[CT]C
BsaWI 352 ACCGGT [AT]^CCGG[AT]
BsmBI 20 GAGACG CGTCTC(1/5)
BsrBI 15 GAGCGG CCGCTC(-3/-3)
Bst1107I 1894 GTATAC GTA^TAC
BstBI 2171 TTCGAA TT^CGAA
BstZ17I 1894 GTATAC GTA^TAC
Eam1105I 3033 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 1504 GGCCGGCC GGCCGG^CC
HaeII 4902 GGCGCT [GA]GCGC^[CT]
Hin1I 656 GACGTC G[GA]^CG[CT]C
HindIII 2811 AAGCTT A^AGCTT
HpaI 4142 GTTAAC GTT^AAC
MfeI 5177 CAATTG C^AATTG
MunI 5177 CAATTG C^AATTG
NaeI 1505 GCCGGC GCC^GGC
NdeI 1578 CATATG CA^TATG
NgoMIV 1505 GCCGGC G^CCGGC
NspV 2171 TTCGAA TT^CGAA
PaeR7I 1316 CTCGAG C^TCGAG
PflFI 3701 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 352 ACCGGT A^CCGGT
PshAI 2742 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 798 ATTAAT AT^TAAT
PvuI 53 CGATCG CGAT^CG
SapI 4738 GCTCTTC GCTCTTC(1/4)
SexAI 5102 ACCAGGT A^CC[AT]GGT
SfuI 2171 TTCGAA TT^CGAA
SmaI 1343 CCCGGG CCC^GGG
SnaBI 162 TACGTA TAC^GTA
Tth111I 3701 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1316 CTCGAG C^TCGAG
XmaCI 1343 CCCGGG C^CCGGG
XmaI 1343 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflII 4710 CTTAAG C^TTAAG
5400 CTTAAG
Alw44I 337 GTGCAC G^TGCAC
476 GTGCAC
ApaI 1139 GGGCCC GGGCC^C
2765 GGGCCC
ApaLI 337 GTGCAC G^TGCAC
476 GTGCAC
AvrII 3716 CCTAGG C^CTAGG
4997 CCTAGG
BbrPI 4260 CACGTG CAC^GTG
4280 CACGTG
BbsI 3147 GAAGAC GAAGAC(2/6)
3707 GTCTTC
BbvCI 4296 GCTGAGG CCTCAGC(-5/-2)
4803 GCTGAGG
BclI 4590 TGATCA T^GATCA
4964 TGATCA
BfrI 4710 CTTAAG C^TTAAG
5400 CTTAAG
BlnI 3716 CCTAGG C^CTAGG
4997 CCTAGG
BpuAI 3147 GAAGAC GAAGAC(2/6)
3707 GTCTTC
BsiEI 53 CGATCG CG[GA][CT]^CG
4061 CGGTCG
BspLU11I 2106 ACATGT A^CATGT
3133 ACATGT
BspMI 2253 ACCTGC ACCTGC(4/8)
4394 ACCTGC
BssSI 871 CTCGTG CACGAG(-5/-1)
3142 CTCGTG
BstEII 348 GGTGACC G^GT[ACGT]ACC
2230 GGTGACC
BstPI 348 GGTGACC G^GT[ACGT]ACC
2230 GGTGACC
BstYI 218 GGATCT [GA]^GATC[CT]
1018 GGATCT
DraI 270 TTTAAA TTT^AAA
5604 TTTAAA
DrdI 1179 GACACATTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5479 GACAGGCCCGTC
EcoNI 311 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1684 CCTCTTGAAGG
EcoO65I 348 GGTGACC G^GT[ACGT]ACC
2230 GGTGACC
EcoT22I 2602 ATGCAT ATGCA^T
5412 ATGCAT
FbaI 4590 TGATCA T^GATCA
4964 TGATCA
HincII 887 GTTGAC GT[CT]^[GA]AC
4142 GTTAAC
HindII 887 GTTGAC GT[CT]^[GA]AC
4142 GTTAAC
MflI 218 GGATCT [GA]^GATC[CT]
1018 GGATCT
NsiI 2602 ATGCAT ATGCA^T
5412 ATGCAT
PflMI 3013 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4043 CCACGGCCTGG
PmaCI 4260 CACGTG CAC^GTG
4280 CACGTG
PmlI 4260 CACGTG CAC^GTG
4280 CACGTG
SacI 1700 GAGCTC GAGCT^C
3139 GAGCTC
SfiI 1504 GGCCGGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4748 GGCCATGGAGGCC
SphI 1810 GCATGC GCATG^C
4493 GCATGC
Van91I 3013 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4043 CCACGGCCTGG
XbaI 1324 TCTAGA T^CTAGA
5005 TCTAGA
XhoII 218 GGATCT [GA]^GATC[CT]
1018 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmnI