##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AhdI AscI
AseI AsnI Asp718I AspEI AspI
BamHI BbvCI BclI BglII BsaBI
BsiWI BsmI BspDI BspLU11I BstBI
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI MamI MfeI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmeI PshBI Psp1406I
RsrII SalI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 4499 AAATTC [GA]^AATT[CT]
AflII 4255 CTTAAG C^TTAAG
AgeI 2557 ACCGGT A^CCGGT
Alw44I 560 GTGCAC G^TGCAC
ApaLI 560 GTGCAC G^TGCAC
ApoI 4499 AAATTC [GA]^AATT[CT]
Asp700I 518 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 984 GGATAC GTATCC(6/5)
BfrI 4255 CTTAAG C^TTAAG
BsrDI 222 GCAATG GCAATG(2/0)
Bst1107I 4036 GTATAC GTA^TAC
BstZ17I 4036 GTATAC GTA^TAC
HindIII 3305 AAGCTT A^AGCTT
KspI 3373 CCGCGG CCGC^GG
MluI 1722 ACGCGT A^CGCGT
NdeI 4041 CATATG CA^TATG
PinAI 2557 ACCGGT A^CCGGT
PshAI 1398 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2538 CGATCG CGAT^CG
SacII 3373 CCGCGG CCGC^GG
ScaI 537 AGTACT AGT^ACT
SphI 4315 GCATGC GCATG^C
StuI 3950 AGGCCT AGG^CCT
XmnI 518 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 127 TCCGGA T^CCGGA
2393 TCCGGA
AflIII 1722 ACGCGT A^C[GA][CT]GT
4191 ACACGT
AviII 315 TGCGCA TGC^GCA
3136 TGCGCA
AvrII 1843 CCTAGG C^CTAGG
2660 CCTAGG
BbeI 411 GGCGCC GGCGC^C
2742 GGCGCC
BbsI 1506 GAAGAC GAAGAC(2/6)
2581 GTCTTC
BlnI 1843 CCTAGG C^CTAGG
2660 CCTAGG
BpuAI 1506 GAAGAC GAAGAC(2/6)
2581 GTCTTC
BseAI 127 TCCGGA T^CCGGA
2393 TCCGGA
BsiEI 1918 CGACCG CG[GA][CT]^CG
2538 CGATCG
BspEI 127 TCCGGA T^CCGGA
2393 TCCGGA
BspHI 648 TCATGA T^CATGA
1976 TCATGA
BssHII 1597 GCGCGC G^CGCGC
2871 GCGCGC
BssSI 265 CACGAG CACGAG(-5/-1)
1387 CACGAG
BstAPI 387 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1120 GCAGGTGGTGC
BstEII 1801 GGTGACC G^GT[ACGT]ACC
4266 GGTTACC
BstPI 1801 GGTGACC G^GT[ACGT]ACC
4266 GGTTACC
DraIII 1264 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2961 CACACGGTG
EcoO65I 1801 GGTGACC G^GT[ACGT]ACC
4266 GGTTACC
FspI 315 TGCGCA TGC^GCA
3136 TGCGCA
KasI 411 GGCGCC G^GCGCC
2742 GGCGCC
MroI 127 TCCGGA T^CCGGA
2393 TCCGGA
NaeI 1465 GCCGGC GCC^GGC
3550 GCCGGC
NarI 411 GGCGCC GG^CGCC
2742 GGCGCC
NcoI 600 CCATGG C^CATGG
3185 CCATGG
NgoMIV 1465 GCCGGC G^CCGGC
3550 GCCGGC
NspI 387 GCATGT [GA]CATG^[CT]
4315 GCATGC
RcaI 648 TCATGA T^CATGA
1976 TCATGA
SacI 997 GAGCTC GAGCT^C
2486 GAGCTC
SapI 994 GAAGAGC GCTCTTC(1/4)
3744 GAAGAGC
SbfI 2736 CCTGCAGG CCTGCA^GG
3945 CCTGCAGG
SexAI 246 ACCTGGT A^CC[AT]GGT
1859 ACCTGGT
SfoI 411 GGCGCC GGC^GCC
2742 GGCGCC
SmaI 696 CCCGGG CCC^GGG
1765 CCCGGG
Sse8387I 2736 CCTGCAGG CCTGCA^GG
3945 CCTGCAGG
XmaCI 696 CCCGGG C^CCGGG
1765 CCCGGG
XmaI 696 CCCGGG C^CCGGG
1765 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII