################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AclI AhdI AscI AseI AsnI Asp718I AspEI AspI BamHI BbvCI BclI BglII BsaBI BsiWI BsmI BspDI BspLU11I BstBI ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KpnI MamI MfeI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AcsI 4499 AAATTC [GA]^AATT[CT] AflII 4255 CTTAAG C^TTAAG AgeI 2557 ACCGGT A^CCGGT Alw44I 560 GTGCAC G^TGCAC ApaLI 560 GTGCAC G^TGCAC ApoI 4499 AAATTC [GA]^AATT[CT] Asp700I 518 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BciVI 984 GGATAC GTATCC(6/5) BfrI 4255 CTTAAG C^TTAAG BsrDI 222 GCAATG GCAATG(2/0) Bst1107I 4036 GTATAC GTA^TAC BstZ17I 4036 GTATAC GTA^TAC HindIII 3305 AAGCTT A^AGCTT KspI 3373 CCGCGG CCGC^GG MluI 1722 ACGCGT A^CGCGT NdeI 4041 CATATG CA^TATG PinAI 2557 ACCGGT A^CCGGT PshAI 1398 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 2538 CGATCG CGAT^CG SacII 3373 CCGCGG CCGC^GG ScaI 537 AGTACT AGT^ACT SphI 4315 GCATGC GCATG^C StuI 3950 AGGCCT AGG^CCT XmnI 518 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccIII 127 TCCGGA T^CCGGA 2393 TCCGGA AflIII 1722 ACGCGT A^C[GA][CT]GT 4191 ACACGT AviII 315 TGCGCA TGC^GCA 3136 TGCGCA AvrII 1843 CCTAGG C^CTAGG 2660 CCTAGG BbeI 411 GGCGCC GGCGC^C 2742 GGCGCC BbsI 1506 GAAGAC GAAGAC(2/6) 2581 GTCTTC BlnI 1843 CCTAGG C^CTAGG 2660 CCTAGG BpuAI 1506 GAAGAC GAAGAC(2/6) 2581 GTCTTC BseAI 127 TCCGGA T^CCGGA 2393 TCCGGA BsiEI 1918 CGACCG CG[GA][CT]^CG 2538 CGATCG BspEI 127 TCCGGA T^CCGGA 2393 TCCGGA BspHI 648 TCATGA T^CATGA 1976 TCATGA BssHII 1597 GCGCGC G^CGCGC 2871 GCGCGC BssSI 265 CACGAG CACGAG(-5/-1) 1387 CACGAG BstAPI 387 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1120 GCAGGTGGTGC BstEII 1801 GGTGACC G^GT[ACGT]ACC 4266 GGTTACC BstPI 1801 GGTGACC G^GT[ACGT]ACC 4266 GGTTACC DraIII 1264 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 2961 CACACGGTG EcoO65I 1801 GGTGACC G^GT[ACGT]ACC 4266 GGTTACC FspI 315 TGCGCA TGC^GCA 3136 TGCGCA KasI 411 GGCGCC G^GCGCC 2742 GGCGCC MroI 127 TCCGGA T^CCGGA 2393 TCCGGA NaeI 1465 GCCGGC GCC^GGC 3550 GCCGGC NarI 411 GGCGCC GG^CGCC 2742 GGCGCC NcoI 600 CCATGG C^CATGG 3185 CCATGG NgoMIV 1465 GCCGGC G^CCGGC 3550 GCCGGC NspI 387 GCATGT [GA]CATG^[CT] 4315 GCATGC RcaI 648 TCATGA T^CATGA 1976 TCATGA SacI 997 GAGCTC GAGCT^C 2486 GAGCTC SapI 994 GAAGAGC GCTCTTC(1/4) 3744 GAAGAGC SbfI 2736 CCTGCAGG CCTGCA^GG 3945 CCTGCAGG SexAI 246 ACCTGGT A^CC[AT]GGT 1859 ACCTGGT SfoI 411 GGCGCC GGC^GCC 2742 GGCGCC SmaI 696 CCCGGG CCC^GGG 1765 CCCGGG Sse8387I 2736 CCTGCAGG CCTGCA^GG 3945 CCTGCAGG XmaCI 696 CCCGGG C^CCGGG 1765 CCCGGG XmaI 696 CCCGGG C^CCGGG 1765 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII