##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AviII BbeI
BbrPI BcgI BclI BfrI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BspEI BspMI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I StuI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 5247 GTCTAC GT^[AC][GT]AC
AccII 372 CGCG CG^CG
AhdI 4547 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2321 ATTAAT AT^TAAT
AsnI 2321 ATTAAT AT^TAAT
AspEI 4547 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1438 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4447 CCTAGG C^CTAGG
BanI 2508 GGTGCC G^G[CT][GA]CC
BlnI 4447 CCTAGG C^CTAGG
BsiEI 1356 CGACCG CG[GA][CT]^CG
BspDI 3645 ATCGAT AT^CGAT
BsrBI 3671 CCGCTC CCGCTC(-3/-3)
BsrFI 210 ACCGGC [GA]^CCGG[CT]
BssSI 713 CTCGTG CACGAG(-5/-1)
BstUI 372 CGCG CG^CG
CfoI 4708 GCGC GCG^C
Cfr10I 210 ACCGGC [GA]^CCGG[CT]
ClaI 3645 ATCGAT AT^CGAT
DraIII 190 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1031 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4547 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5941 ATGCAT ATGCA^T
HhaI 4708 GCGC GCG^C
HinP1I 4708 GCGC G^CGC
MvnI 372 CGCG CG^CG
NsiI 5941 ATGCAT ATGCA^T
PflFI 1438 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2321 ATTAAT AT^TAAT
SacI 2062 GAGCTC GAGCT^C
SexAI 3060 ACCAGGT A^CC[AT]GGT
SpeI 5628 ACTAGT A^CTAGT
SwaI 5669 ATTTAAAT ATTT^AAAT
Tth111I 1438 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2230 CCATCCCAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Asp700I 4767 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6050 GAACAACTTC
AvaI 1130 CCCGAG C^[CT]CG[GA]G
1147 CTCGGG
BamHI 3627 GGATCC G^GATCC
4443 GGATCC
BglI 3214 GCCCTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3661 GCCACCATGGC
BlpI 311 GCTCAGC GC^T[ACGT]AGC
4734 GCTCAGC
Bpu1102I 311 GCTCAGC GC^T[ACGT]AGC
4734 GCTCAGC
BsmI 3236 GAATGC GAATGC(1/-1)
2557 GCATTC
BsoBI 1130 CCCGAG C^[CT]CG[GA]G
1147 CTCGGG
BspHI 1246 TCATGA T^CATGA
5767 TCATGA
BstXI 2124 CCACTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3383 CCAGACAGTTGG
CelII 311 GCTCAGC GC^T[ACGT]AGC
4734 GCTCAGC
EcoNI 797 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5606 CCTTCTGGAGG
FauI 1227 CCCGC CCCGC(4/6)
1992 CCCGC
NheI 1192 GCTAGC G^CTAGC
4804 GCTAGC
RcaI 1246 TCATGA T^CATGA
5767 TCATGA
SapI 1271 GAAGAGC GCTCTTC(1/4)
2073 GCTCTTC
ScaI 1855 AGTACT AGT^ACT
4937 AGTACT
SmlI 2067 CTCAAG C^T[CT][GA]AG
3700 CTCAAG
SphI 2432 GCATGC GCATG^C
3497 GCATGC
XbaI 4392 TCTAGA T^CTAGA
4855 TCTAGA
XmnI 4767 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6050 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII