##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AspI AviII BbeI
BbrPI BfrI BsaAI BsaBI BsiEI
BsiWI Bsp1407I BspDI BsrBI BsrGI
BssSI Bst1107I BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoRV FseI
FspI HindIII HpaI KasI KspI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 4856 GGTACC G^GTACC
AccIII 247 TCCGGA T^CCGGA
Alw44I 8 GTGCAC G^TGCAC
Aor51HI 750 AGCGCT AGC^GCT
ApaLI 8 GTGCAC G^TGCAC
Asp718I 4856 GGTACC G^GTACC
BcgI 1963 CGACTGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4467 GTATCC GTATCC(6/5)
BclI 5054 TGATCA T^GATCA
BseAI 247 TCCGGA T^CCGGA
BsmBI 4413 CGTCTC CGTCTC(1/5)
BspEI 247 TCCGGA T^CCGGA
BspLU11I 4246 ACATGT A^CATGT
BsrFI 1129 ACCGGC [GA]^CCGG[CT]
BssHII 63 GCGCGC G^CGCGC
BstBI 1928 TTCGAA TT^CGAA
Cfr10I 1129 ACCGGC [GA]^CCGG[CT]
Eco47III 750 AGCGCT AGC^GCT
EcoRI 3274 GAATTC G^AATTC
EcoT22I 4908 ATGCAT ATGCA^T
FauI 86 GCGGG CCCGC(4/6)
FbaI 5054 TGATCA T^GATCA
HincII 1961 GTCGAC GT[CT]^[GA]AC
HindII 1961 GTCGAC GT[CT]^[GA]AC
KpnI 4856 GGTACC GGTAC^C
MroI 247 TCCGGA T^CCGGA
NsiI 4908 ATGCAT ATGCA^T
NspV 1928 TTCGAA TT^CGAA
SalI 1961 GTCGAC G^TCGAC
SfuI 1928 TTCGAA TT^CGAA
XbaI 1568 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 62 CGCG CG^CG
64 CGCG
AcsI 3274 GAATTC [GA]^AATT[CT]
3809 AAATTC
AflIII 4246 ACATGT A^C[GA][CT]GT
5464 ACACGT
AhdI 1482 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3603 GACTTTCAGTC
ApaI 2707 GGGCCC GGGCC^C
2762 GGGCCC
ApoI 3274 GAATTC [GA]^AATT[CT]
3809 AAATTC
AspEI 1482 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3603 GACTTTCAGTC
AvrII 1159 CCTAGG C^CTAGG
4758 CCTAGG
BamHI 3043 GGATCC G^GATCC
3621 GGATCC
BanI 1173 GGTGCC G^G[CT][GA]CC
4856 GGTACC
BglI 545 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4499 GCCACTGAGGC
BlnI 1159 CCTAGG C^CTAGG
4758 CCTAGG
BsaWI 247 TCCGGA [AT]^CCGG[AT]
862 ACCGGA
BsmI 1797 GAATGC GAATGC(1/-1)
3111 GAATGC
BspHI 4344 TCATGA T^CATGA
4626 TCATGA
BstAPI 10 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2218 GCAAGCCCTGC
BstUI 62 CGCG CG^CG
64 CGCG
BstXI 2610 CCATAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4815 CCACACTGGTGG
DrdI 1113 GACAGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2041 GACGCTTGGGTC
Eam1105I 1482 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3603 GACTTTCAGTC
HaeII 750 AGCGCT [GA]GCGC^[CT]
5405 GGCGCT
MvnI 62 CGCG CG^CG
64 CGCG
PaeR7I 1151 CTCGAG C^TCGAG
4397 CTCGAG
RcaI 4344 TCATGA T^CATGA
4626 TCATGA
SapI 1429 GCTCTTC GCTCTTC(1/4)
3980 GCTCTTC
XcmI 775 CCAGTGTTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3351 CCATGGGAGACATGG
XhoI 1151 CTCGAG C^TCGAG
4397 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AocI Asp700I AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI