##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AvaI AviII AvrII
BbeI BbrPI BcgI BlnI BsaHI
BseAI BsiEI BsiWI BsmBI BsoBI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstUI Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI Hin1I HindIII
KasI KspI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 520 GGTACC G^GTACC
ApaI 2488 GGGCCC GGGCC^C
Asp700I 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 520 GGTACC G^GTACC
BalI 2967 TGGCCA TGG^CCA
BamHI 562 GGATCC G^GATCC
BanI 520 GGTACC G^G[CT][GA]CC
BbvCI 883 GCTGAGG CCTCAGC(-5/-2)
BciVI 1788 GTATCC GTATCC(6/5)
BsaAI 2311 CACGTA [CT]AC^GT[GA]
BsaBI 3451 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 517 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3616 TGTACA T^GTACA
BspDI 4083 ATCGAT AT^CGAT
BspLU11I 2732 ACATGT A^CATGT
BspMI 2449 ACCTGC ACCTGC(4/8)
BsrDI 4483 GCAATG GCAATG(2/0)
BsrGI 3616 TGTACA T^GTACA
Bst1107I 2314 GTATAC GTA^TAC
BstEII 978 GGTAACC G^GT[ACGT]ACC
BstPI 978 GGTAACC G^GT[ACGT]ACC
BstZ17I 2314 GTATAC GTA^TAC
ClaI 4083 ATCGAT AT^CGAT
DsaI 653 CCGTGG C^C[GA][CT]GG
EcoNI 2610 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 978 GGTAACC G^GT[ACGT]ACC
EcoRI 4150 GAATTC G^AATTC
EcoRV 2921 GATATC GAT^ATC
EcoT22I 1782 ATGCAT ATGCA^T
FauI 3107 CCCGC CCCGC(4/6)
HaeII 743 GGCGCT [GA]GCGC^[CT]
HgaI 4390 GCGTC GACGC(5/10)
HincII 121 GTTAAC GT[CT]^[GA]AC
HindII 121 GTTAAC GT[CT]^[GA]AC
HpaI 121 GTTAAC GTT^AAC
KpnI 520 GGTACC GGTAC^C
MamI 3451 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2967 TGGCCA TGG^CCA
MscI 2967 TGGCCA TGG^CCA
NdeI 4712 CATATG CA^TATG
NheI 1123 GCTAGC G^CTAGC
NsiI 1782 ATGCAT ATGCA^T
PacI 3121 TTAATTAA TTAAT^TAA
PpuMI 782 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 673 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2347 GAGCTC GAGCT^C
SbfI 2450 CCTGCAGG CCTGCA^GG
Sse8387I 2450 CCTGCAGG CCTGCA^GG
SspBI 3616 TGTACA T^GTACA
SwaI 3727 ATTTAAAT ATTT^AAAT
XbaI 3030 TCTAGA T^CTAGA
XmnI 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2314 GTATAC GT^[AC][GT]AC
5046 GTAGAC
AflIII 2310 ACACGT A^C[GA][CT]GT
2732 ACATGT
Alw44I 1877 GTGCAC G^TGCAC
2155 GTGCAC
AlwNI 1235 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2605 CAGCTCCTG
AocI 706 CCTGAGG CC^T[ACGT]AGG
2614 CCTCAGG
ApaLI 1877 GTGCAC G^TGCAC
2155 GTGCAC
AspI 481 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
570 GACAAAGTC
BbsI 680 GTCTTC GAAGAC(2/6)
3075 GTCTTC
BclI 165 TGATCA T^GATCA
1046 TGATCA
BcnI 497 CCCGG CC^[GC]GG
507 CCGGG
BglI 874 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3110 GCCATTGGGGC
BlpI 2121 GCTCAGC GC^T[ACGT]AGC
2556 GCTGAGC
Bpu1102I 2121 GCTCAGC GC^T[ACGT]AGC
2556 GCTGAGC
BpuAI 680 GTCTTC GAAGAC(2/6)
3075 GTCTTC
BsaI 1677 GAGACC GGTCTC(1/5)
3156 GGTCTC
BseRI 2138 CTCCTC GAGGAG(10/8)
709 GAGGAG
BsmI 2798 GCATTC GAATGC(1/-1)
4941 GCATTC
BstXI 2344 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3844 CCAGAAGCCTGG
Bsu36I 706 CCTGAGG CC^T[ACGT]AGG
2614 CCTCAGG
CelII 2121 GCTCAGC GC^T[ACGT]AGC
2556 GCTGAGC
DraII 782 AGGACCT [GA]G^G[ACGT]CC[CT]
2488 GGGCCCC
DraIII 1625 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3312 CACTTGGTG
EaeI 840 TGGCCG [CT]^GGCC[GA]
2967 TGGCCA
Eco81I 706 CCTGAGG CC^T[ACGT]AGG
2614 CCTCAGG
EcoO109I 782 AGGACCT [GA]G^G[ACGT]CC[CT]
2488 GGGCCCC
FbaI 165 TGATCA T^GATCA
1046 TGATCA
MaeII 2312 ACGT A^CGT
4954 ACGT
MfeI 821 CAATTG C^AATTG
3915 CAATTG
MspA1I 1113 CAGCTG C[AC]G^C[GT]G
1909 CAGCTG
MunI 821 CAATTG C^AATTG
3915 CAATTG
NciI 497 CCCGG CC^[GC]GG
507 CCGGG
PflFI 481 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
570 GACAAAGTC
PflMI 1624 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2522 CCAGTTTGTGG
PvuII 1113 CAGCTG CAG^CTG
1909 CAGCTG
SpeI 1895 ACTAGT A^CTAGT
2469 ACTAGT
SphI 2903 GCATGC GCATG^C
4664 GCATGC
SspI 3875 AATATT AAT^ATT
5001 AATATT
TaqI 152 TCGA T^CGA
4084 TCGA
Tsp45I 144 GTCAC ^GT[GC]AC
1385 GTCAC
Tth111I 481 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
570 GACAAAGTC
TthHB8I 152 TCGA T^CGA
4084 TCGA
Van91I 1624 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2522 CCAGTTTGTGG
XcmI 1617 CCAGAATCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4469 CCATCATTACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AspHI AvaII BanII
BbvI BfaI BfrI BglII BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII