################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AvaI AviII AvrII BbeI BbrPI BcgI BlnI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstUI Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI Hin1I HindIII KasI KspI MluI MroI MvnI NaeI NarI NcoI NgoMIV NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 520 GGTACC G^GTACC ApaI 2488 GGGCCC GGGCC^C Asp700I 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 520 GGTACC G^GTACC BalI 2967 TGGCCA TGG^CCA BamHI 562 GGATCC G^GATCC BanI 520 GGTACC G^G[CT][GA]CC BbvCI 883 GCTGAGG CCTCAGC(-5/-2) BciVI 1788 GTATCC GTATCC(6/5) BsaAI 2311 CACGTA [CT]AC^GT[GA] BsaBI 3451 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 517 TCCGGT [AT]^CCGG[AT] Bsp1407I 3616 TGTACA T^GTACA BspDI 4083 ATCGAT AT^CGAT BspLU11I 2732 ACATGT A^CATGT BspMI 2449 ACCTGC ACCTGC(4/8) BsrDI 4483 GCAATG GCAATG(2/0) BsrGI 3616 TGTACA T^GTACA Bst1107I 2314 GTATAC GTA^TAC BstEII 978 GGTAACC G^GT[ACGT]ACC BstPI 978 GGTAACC G^GT[ACGT]ACC BstZ17I 2314 GTATAC GTA^TAC ClaI 4083 ATCGAT AT^CGAT DsaI 653 CCGTGG C^C[GA][CT]GG EcoNI 2610 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 978 GGTAACC G^GT[ACGT]ACC EcoRI 4150 GAATTC G^AATTC EcoRV 2921 GATATC GAT^ATC EcoT22I 1782 ATGCAT ATGCA^T FauI 3107 CCCGC CCCGC(4/6) HaeII 743 GGCGCT [GA]GCGC^[CT] HgaI 4390 GCGTC GACGC(5/10) HincII 121 GTTAAC GT[CT]^[GA]AC HindII 121 GTTAAC GT[CT]^[GA]AC HpaI 121 GTTAAC GTT^AAC KpnI 520 GGTACC GGTAC^C MamI 3451 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2967 TGGCCA TGG^CCA MscI 2967 TGGCCA TGG^CCA NdeI 4712 CATATG CA^TATG NheI 1123 GCTAGC G^CTAGC NsiI 1782 ATGCAT ATGCA^T PacI 3121 TTAATTAA TTAAT^TAA PpuMI 782 AGGACCT [GA]G^G[AT]CC[CT] PshAI 673 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2347 GAGCTC GAGCT^C SbfI 2450 CCTGCAGG CCTGCA^GG Sse8387I 2450 CCTGCAGG CCTGCA^GG SspBI 3616 TGTACA T^GTACA SwaI 3727 ATTTAAAT ATTT^AAAT XbaI 3030 TCTAGA T^CTAGA XmnI 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccI 2314 GTATAC GT^[AC][GT]AC 5046 GTAGAC AflIII 2310 ACACGT A^C[GA][CT]GT 2732 ACATGT Alw44I 1877 GTGCAC G^TGCAC 2155 GTGCAC AlwNI 1235 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2605 CAGCTCCTG AocI 706 CCTGAGG CC^T[ACGT]AGG 2614 CCTCAGG ApaLI 1877 GTGCAC G^TGCAC 2155 GTGCAC AspI 481 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 570 GACAAAGTC BbsI 680 GTCTTC GAAGAC(2/6) 3075 GTCTTC BclI 165 TGATCA T^GATCA 1046 TGATCA BcnI 497 CCCGG CC^[GC]GG 507 CCGGG BglI 874 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3110 GCCATTGGGGC BlpI 2121 GCTCAGC GC^T[ACGT]AGC 2556 GCTGAGC Bpu1102I 2121 GCTCAGC GC^T[ACGT]AGC 2556 GCTGAGC BpuAI 680 GTCTTC GAAGAC(2/6) 3075 GTCTTC BsaI 1677 GAGACC GGTCTC(1/5) 3156 GGTCTC BseRI 2138 CTCCTC GAGGAG(10/8) 709 GAGGAG BsmI 2798 GCATTC GAATGC(1/-1) 4941 GCATTC BstXI 2344 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3844 CCAGAAGCCTGG Bsu36I 706 CCTGAGG CC^T[ACGT]AGG 2614 CCTCAGG CelII 2121 GCTCAGC GC^T[ACGT]AGC 2556 GCTGAGC DraII 782 AGGACCT [GA]G^G[ACGT]CC[CT] 2488 GGGCCCC DraIII 1625 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3312 CACTTGGTG EaeI 840 TGGCCG [CT]^GGCC[GA] 2967 TGGCCA Eco81I 706 CCTGAGG CC^T[ACGT]AGG 2614 CCTCAGG EcoO109I 782 AGGACCT [GA]G^G[ACGT]CC[CT] 2488 GGGCCCC FbaI 165 TGATCA T^GATCA 1046 TGATCA MaeII 2312 ACGT A^CGT 4954 ACGT MfeI 821 CAATTG C^AATTG 3915 CAATTG MspA1I 1113 CAGCTG C[AC]G^C[GT]G 1909 CAGCTG MunI 821 CAATTG C^AATTG 3915 CAATTG NciI 497 CCCGG CC^[GC]GG 507 CCGGG PflFI 481 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 570 GACAAAGTC PflMI 1624 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2522 CCAGTTTGTGG PvuII 1113 CAGCTG CAG^CTG 1909 CAGCTG SpeI 1895 ACTAGT A^CTAGT 2469 ACTAGT SphI 2903 GCATGC GCATG^C 4664 GCATGC SspI 3875 AATATT AAT^ATT 5001 AATATT TaqI 152 TCGA T^CGA 4084 TCGA Tsp45I 144 GTCAC ^GT[GC]AC 1385 GTCAC Tth111I 481 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 570 GACAAAGTC TthHB8I 152 TCGA T^CGA 4084 TCGA Van91I 1624 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2522 CCAGTTTGTGG XcmI 1617 CCAGAATCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4469 CCATCATTACTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI ApoI AspHI AvaII BanII BbvI BfaI BfrI BglII BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII