##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AgeI        AhdI        Aor51HI     AscI        AseI        
AsnI        AspEI       AvaI        AviII       AvrII       
BbeI        BbrPI       BcgI        BlnI        BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
BstAPI      BstBI       BstUI       Cfr10I      CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      FseI        FspI        Hin1I       HindIII     
KasI        KspI        MluI        MroI        MvnI        
NaeI        NarI        NcoI        NgoMIV      NotI        
NruI        NspV        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        520  GGTACC               G^GTACC
ApaI         2488  GGGCCC               GGGCC^C
Asp700I       922  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       520  GGTACC               G^GTACC
BalI         2967  TGGCCA               TGG^CCA
BamHI         562  GGATCC               G^GATCC
BanI          520  GGTACC               G^G[CT][GA]CC
BbvCI         883  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1788  GTATCC               GTATCC(6/5)
BsaAI        2311  CACGTA               [CT]AC^GT[GA]
BsaBI        3451  GATAGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         517  TCCGGT               [AT]^CCGG[AT]
Bsp1407I     3616  TGTACA               T^GTACA
BspDI        4083  ATCGAT               AT^CGAT
BspLU11I     2732  ACATGT               A^CATGT
BspMI        2449  ACCTGC               ACCTGC(4/8)
BsrDI        4483  GCAATG               GCAATG(2/0)
BsrGI        3616  TGTACA               T^GTACA
Bst1107I     2314  GTATAC               GTA^TAC
BstEII        978  GGTAACC              G^GT[ACGT]ACC
BstPI         978  GGTAACC              G^GT[ACGT]ACC
BstZ17I      2314  GTATAC               GTA^TAC
ClaI         4083  ATCGAT               AT^CGAT
DsaI          653  CCGTGG               C^C[GA][CT]GG
EcoNI        2610  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       978  GGTAACC              G^GT[ACGT]ACC
EcoRI        4150  GAATTC               G^AATTC
EcoRV        2921  GATATC               GAT^ATC
EcoT22I      1782  ATGCAT               ATGCA^T
FauI         3107  CCCGC                CCCGC(4/6)
HaeII         743  GGCGCT               [GA]GCGC^[CT]
HgaI         4390  GCGTC                GACGC(5/10)
HincII        121  GTTAAC               GT[CT]^[GA]AC
HindII        121  GTTAAC               GT[CT]^[GA]AC
HpaI          121  GTTAAC               GTT^AAC
KpnI          520  GGTACC               GGTAC^C
MamI         3451  GATAGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2967  TGGCCA               TGG^CCA
MscI         2967  TGGCCA               TGG^CCA
NdeI         4712  CATATG               CA^TATG
NheI         1123  GCTAGC               G^CTAGC
NsiI         1782  ATGCAT               ATGCA^T
PacI         3121  TTAATTAA             TTAAT^TAA
PpuMI         782  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         673  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2347  GAGCTC               GAGCT^C
SbfI         2450  CCTGCAGG             CCTGCA^GG
Sse8387I     2450  CCTGCAGG             CCTGCA^GG
SspBI        3616  TGTACA               T^GTACA
SwaI         3727  ATTTAAAT             ATTT^AAAT
XbaI         3030  TCTAGA               T^CTAGA
XmnI          922  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccI         2314  GTATAC               GT^[AC][GT]AC
             5046  GTAGAC              
AflIII       2310  ACACGT               A^C[GA][CT]GT
             2732  ACATGT              
Alw44I       1877  GTGCAC               G^TGCAC
             2155  GTGCAC              
AlwNI        1235  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2605  CAGCTCCTG           
AocI          706  CCTGAGG              CC^T[ACGT]AGG
             2614  CCTCAGG             
ApaLI        1877  GTGCAC               G^TGCAC
             2155  GTGCAC              
AspI          481  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              570  GACAAAGTC           
BbsI          680  GTCTTC               GAAGAC(2/6)
             3075  GTCTTC              
BclI          165  TGATCA               T^GATCA
             1046  TGATCA              
BcnI          497  CCCGG                CC^[GC]GG
              507  CCGGG               
BglI          874  GCCTGGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3110  GCCATTGGGGC         
BlpI         2121  GCTCAGC              GC^T[ACGT]AGC
             2556  GCTGAGC             
Bpu1102I     2121  GCTCAGC              GC^T[ACGT]AGC
             2556  GCTGAGC             
BpuAI         680  GTCTTC               GAAGAC(2/6)
             3075  GTCTTC              
BsaI         1677  GAGACC               GGTCTC(1/5)
             3156  GGTCTC              
BseRI        2138  CTCCTC               GAGGAG(10/8)
              709  GAGGAG              
BsmI         2798  GCATTC               GAATGC(1/-1)
             4941  GCATTC              
BstXI        2344  CCAGAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3844  CCAGAAGCCTGG        
Bsu36I        706  CCTGAGG              CC^T[ACGT]AGG
             2614  CCTCAGG             
CelII        2121  GCTCAGC              GC^T[ACGT]AGC
             2556  GCTGAGC             
DraII         782  AGGACCT              [GA]G^G[ACGT]CC[CT]
             2488  GGGCCCC             
DraIII       1625  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3312  CACTTGGTG           
EaeI          840  TGGCCG               [CT]^GGCC[GA]
             2967  TGGCCA              
Eco81I        706  CCTGAGG              CC^T[ACGT]AGG
             2614  CCTCAGG             
EcoO109I      782  AGGACCT              [GA]G^G[ACGT]CC[CT]
             2488  GGGCCCC             
FbaI          165  TGATCA               T^GATCA
             1046  TGATCA              
MaeII        2312  ACGT                 A^CGT
             4954  ACGT                
MfeI          821  CAATTG               C^AATTG
             3915  CAATTG              
MspA1I       1113  CAGCTG               C[AC]G^C[GT]G
             1909  CAGCTG              
MunI          821  CAATTG               C^AATTG
             3915  CAATTG              
NciI          497  CCCGG                CC^[GC]GG
              507  CCGGG               
PflFI         481  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              570  GACAAAGTC           
PflMI        1624  CCACATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2522  CCAGTTTGTGG         
PvuII        1113  CAGCTG               CAG^CTG
             1909  CAGCTG              
SpeI         1895  ACTAGT               A^CTAGT
             2469  ACTAGT              
SphI         2903  GCATGC               GCATG^C
             4664  GCATGC              
SspI         3875  AATATT               AAT^ATT
             5001  AATATT              
TaqI          152  TCGA                 T^CGA
             4084  TCGA                
Tsp45I        144  GTCAC                ^GT[GC]AC
             1385  GTCAC               
Tth111I       481  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              570  GACAAAGTC           
TthHB8I       152  TCGA                 T^CGA
             4084  TCGA                
Van91I       1624  CCACATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2522  CCAGTTTGTGG         
XcmI         1617  CCAGAATCCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4469  CCATCATTACTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        ApoI        AspHI       AvaII       BanII       
BbvI        BfaI        BfrI        BglII       BmyI        
BpmI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XhoII