##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccII AclI Aor51HI AscI AseI
AsnI BamHI BbeI BbrPI BsaAI
BsaBI BsiWI BsmBI BspDI BssHII
BstAPI BstUI ClaI CpoI Eco47III
EcoRV FseI KasI KspI MamI
MluI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SfiI SfoI SnaBI
SpeI SphI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 4384 GACGTC GACGT^C
Acc65I 1717 GGTACC G^GTACC
AccIII 376 TCCGGA T^CCGGA
AflII 3354 CTTAAG C^TTAAG
AgeI 2327 ACCGGT A^CCGGT
Alw44I 630 GTGCAC G^TGCAC
AocI 4635 CCTCAGG CC^T[ACGT]AGG
ApaLI 630 GTGCAC G^TGCAC
Asp700I 3797 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1717 GGTACC G^GTACC
AviII 4413 TGCGCA TGC^GCA
AvrII 3003 CCTAGG C^CTAGG
BbvCI 3138 GCTGAGG CCTCAGC(-5/-2)
BclI 4587 TGATCA T^GATCA
BfrI 3354 CTTAAG C^TTAAG
BglI 520 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2799 AGATCT A^GATCT
BlnI 3003 CCTAGG C^CTAGG
BseAI 376 TCCGGA T^CCGGA
BsiEI 647 CGGCCG CG[GA][CT]^CG
Bsp1407I 1729 TGTACA T^GTACA
BspEI 376 TCCGGA T^CCGGA
BspLU11I 4192 ACATGT A^CATGT
BsrBI 3737 GAGCGG CCGCTC(-3/-3)
BsrFI 2327 ACCGGT [GA]^CCGG[CT]
BsrGI 1729 TGTACA T^GTACA
Bst1107I 3875 GTATAC GTA^TAC
BstZ17I 3875 GTATAC GTA^TAC
Bsu36I 4635 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2327 ACCGGT [GA]^CCGG[CT]
EagI 647 CGGCCG C^GGCCG
EarI 4760 CTCTTC CTCTTC(1/4)
EclXI 647 CGGCCG C^GGCCG
Eco52I 647 CGGCCG C^GGCCG
Eco81I 4635 CCTCAGG CC^T[ACGT]AGG
EcoRI 2705 GAATTC G^AATTC
EcoT22I 1888 ATGCAT ATGCA^T
FbaI 4587 TGATCA T^GATCA
FspI 4413 TGCGCA TGC^GCA
HaeII 1940 GGCGCT [GA]GCGC^[CT]
HindIII 732 AAGCTT A^AGCTT
HpaI 1866 GTTAAC GTT^AAC
KpnI 1717 GGTACC GGTAC^C
Ksp632I 4760 CTCTTC CTCTTC(1/4)
MfeI 4070 CAATTG C^AATTG
MroI 376 TCCGGA T^CCGGA
MspA1I 2867 CAGCTG C[AC]G^C[GT]G
MunI 4070 CAATTG C^AATTG
NsiI 1888 ATGCAT ATGCA^T
PinAI 2327 ACCGGT A^CCGGT
PvuII 2867 CAGCTG CAG^CTG
SbfI 1425 CCTGCAGG CCTGCA^GG
SgrAI 2326 CACCGGTG C[GA]^CCGG[CT]G
SmaI 671 CCCGGG CCC^GGG
Sse8387I 1425 CCTGCAGG CCTGCA^GG
SspBI 1729 TGTACA T^GTACA
TfiI 3175 GATTC G^A[AT]TC
XmaCI 671 CCCGGG C^CCGGG
XmaI 671 CCCGGG C^CCGGG
XmnI 3797 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 4384 GACGTC G[GA]^CG[CT]C
4860 GGCGTC
AflIII 4192 ACATGT A^C[GA][CT]GT
4398 ACGTGT
AhdI 3920 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4151 GACGTGGTGTC
AspEI 3920 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4151 GACGTGGTGTC
BcgI 3829 CGAGTCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2455 GCATGGTCTTCG
BsaHI 4384 GACGTC G[GA]^CG[CT]C
4860 GGCGTC
BsgI 489 CTGCAC GTGCAG(16/14)
741 CTGCAC
BsmI 183 GAATGC GAATGC(1/-1)
1816 GCATTC
BspHI 1462 TCATGA T^CATGA
4269 TCATGA
BsrDI 516 CATTGC GCAATG(2/0)
4137 GCAATG
BssSI 2006 CACGAG CACGAG(-5/-1)
4350 CTCGTG
BstBI 852 TTCGAA TT^CGAA
4335 TTCGAA
BstEII 3679 GGTCACC G^GT[ACGT]ACC
4773 GGTGACC
BstPI 3679 GGTCACC G^GT[ACGT]ACC
4773 GGTGACC
DraIII 2006 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4129 CACAATGTG
DrdI 44 GACCACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2917 GACTACAGAGTC
Eam1105I 3920 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4151 GACGTGGTGTC
EcoO65I 3679 GGTCACC G^GT[ACGT]ACC
4773 GGTGACC
FauI 1652 CCCGC CCCGC(4/6)
3739 GCGGG
Hin1I 4384 GACGTC G[GA]^CG[CT]C
4860 GGCGTC
NspV 852 TTCGAA TT^CGAA
4335 TTCGAA
PflMI 1055 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1337 CCACGTCCTGG
PshAI 1825 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2888 GACTAGGGTC
RcaI 1462 TCATGA T^CATGA
4269 TCATGA
ScaI 1351 AGTACT AGT^ACT
2558 AGTACT
SexAI 2929 ACCAGGT A^CC[AT]GGT
3957 ACCAGGT
SfuI 852 TTCGAA TT^CGAA
4335 TTCGAA
Van91I 1055 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1337 CCACGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII