##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AccI AgeI AscI AseI AsnI
AvrII BclI BlnI BsiWI BspDI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI DraI EcoRI EcoRV FbaI
FseI HpaI KspI MfeI MluI
MunI NotI NspV PacI PinAI
PmeI PshBI PvuI RcaI SacII
SalI SapI SbfI SfiI SfuI
SnaBI Sse8387I SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 2762 AACGTT AA^CGTT
AflII 1880 CTTAAG C^TTAAG
AflIII 5055 ACATGT A^C[GA][CT]GT
AhdI 4556 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4556 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5129 TGCGCA TGC^GCA
BamHI 1715 GGATCC G^GATCC
BbeI 5393 GGCGCC GGCGC^C
BbrPI 4672 CACGTG CAC^GTG
BfrI 1880 CTTAAG C^TTAAG
BglII 637 AGATCT A^GATCT
BsaBI 2488 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 5055 ACATGT A^CATGT
CpoI 4891 CGGTCCG CG^G[AT]CCG
EagI 1278 CGGCCG C^GGCCG
Eam1105I 4556 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1278 CGGCCG C^GGCCG
Eco52I 1278 CGGCCG C^GGCCG
EcoT22I 4621 ATGCAT ATGCA^T
FspI 5129 TGCGCA TGC^GCA
HindIII 5844 AAGCTT A^AGCTT
KasI 5393 GGCGCC G^GCGCC
MamI 2488 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 5393 GGCGCC GG^CGCC
NdeI 6380 CATATG CA^TATG
NheI 5286 GCTAGC G^CTAGC
NruI 3726 TCGCGA TCG^CGA
NsiI 4621 ATGCAT ATGCA^T
PaeR7I 726 CTCGAG C^TCGAG
PmaCI 4672 CACGTG CAC^GTG
PmlI 4672 CACGTG CAC^GTG
Psp1406I 2762 AACGTT AA^CGTT
RsrII 4891 CGGTCCG CG^G[AT]CCG
ScaI 4426 AGTACT AGT^ACT
SfoI 5393 GGCGCC GGC^GCC
SgrAI 1208 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 2522 ACTAGT A^CTAGT
SphI 3989 GCATGC GCATG^C
XbaI 2443 TCTAGA T^CTAGA
XhoI 726 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 2745 GACGTC GACGT^C
5655 GACGTC
Acc65I 1380 GGTACC G^GTACC
5501 GGTACC
AcsI 1649 GAATTT [GA]^AATT[CT]
3342 GAATTT
ApoI 1649 GAATTT [GA]^AATT[CT]
3342 GAATTT
Asp700I 1444 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4966 GAAGGGCTTC
Asp718I 1380 GGTACC G^GTACC
5501 GGTACC
AspI 2705 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4603 GACAAGGTC
BbvCI 4402 GCTGAGG CCTCAGC(-5/-2)
5061 GCTGAGG
BcgI 712 CGAGAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4055 CGACAGCTCTGC
BsaAI 4261 TACGTG [CT]AC^GT[GA]
4672 CACGTG
Bsp1407I 1853 TGTACA T^GTACA
4832 TGTACA
BsrGI 1853 TGTACA T^GTACA
4832 TGTACA
BstEII 2330 GGTGACC G^GT[ACGT]ACC
4434 GGTCACC
BstPI 2330 GGTGACC G^GT[ACGT]ACC
4434 GGTCACC
EcoO65I 2330 GGTGACC G^GT[ACGT]ACC
4434 GGTCACC
HincII 560 GTCAAC GT[CT]^[GA]AC
4564 GTCAAC
HindII 560 GTCAAC GT[CT]^[GA]AC
4564 GTCAAC
KpnI 1380 GGTACC GGTAC^C
5501 GGTACC
PflFI 2705 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4603 GACAAGGTC
PshAI 1663 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3085 GACTCTGGTC
SspBI 1853 TGTACA T^GTACA
4832 TGTACA
Tth111I 2705 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4603 GACAAGGTC
XmnI 1444 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4966 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI