##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AscI AseI AsnI AspI
BamHI BbrPI BbvCI BclI BsaBI
BsaWI BseAI BsmBI BspDI BspEI
BspHI BssHII BstBI ClaI CpoI
DraIII EcoRV FbaI HindIII KspI
MamI MluI MroI NdeI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfuI SgrAI Sse8387I
Tth111I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1269 GGTACC G^GTACC
AccI 1513 GTATAC GT^[AC][GT]AC
AflII 5008 CTTAAG C^TTAAG
AhdI 5103 GACACTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2461 AGCGCT AGC^GCT
Asp718I 1269 GGTACC G^GTACC
AspEI 5103 GACACTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 371 TGCGCA TGC^GCA
BbeI 235 GGCGCC GGCGC^C
BcgI 3751 GCATAGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1766 GGATAC GTATCC(6/5)
BfrI 5008 CTTAAG C^TTAAG
BglII 99 AGATCT A^GATCT
BsaAI 1516 TACGTA [CT]AC^GT[GA]
BsiEI 383 CGGCCG CG[GA][CT]^CG
BsiWI 1518 CGTACG C^GTACG
BspLU11I 4010 ACATGT A^CATGT
BsrBI 1406 CCGCTC CCGCTC(-3/-3)
BssSI 1822 CACGAG CACGAG(-5/-1)
Bst1107I 1513 GTATAC GTA^TAC
BstEII 5145 GGTTACC G^GT[ACGT]ACC
BstPI 5145 GGTTACC G^GT[ACGT]ACC
BstZ17I 1513 GTATAC GTA^TAC
DrdI 2274 GACTTCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 383 CGGCCG C^GGCCG
Eam1105I 5103 GACACTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 383 CGGCCG C^GGCCG
Eco47III 2461 AGCGCT AGC^GCT
Eco52I 383 CGGCCG C^GGCCG
EcoO65I 5145 GGTTACC G^GT[ACGT]ACC
EcoRI 5571 GAATTC G^AATTC
EcoT22I 3674 ATGCAT ATGCA^T
FseI 380 GGCCGGCC GGCCGG^CC
FspI 371 TGCGCA TGC^GCA
HgaI 3658 GACGC GACGC(5/10)
KasI 235 GGCGCC G^GCGCC
KpnI 1269 GGTACC GGTAC^C
MfeI 222 CAATTG C^AATTG
MunI 222 CAATTG C^AATTG
NarI 235 GGCGCC GG^CGCC
NsiI 3674 ATGCAT ATGCA^T
PaeR7I 952 CTCGAG C^TCGAG
PflMI 47 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4975 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2062 GAGCTC GAGCT^C
SexAI 4281 ACCAGGT A^CC[AT]GGT
SfoI 235 GGCGCC GGC^GCC
SmaI 476 CCCGGG CCC^GGG
SnaBI 1516 TACGTA TAC^GTA
SphI 1600 GCATGC GCATG^C
StuI 1185 AGGCCT AGG^CCT
SwaI 4557 ATTTAAAT ATTT^AAAT
Van91I 47 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2642 TCTAGA T^CTAGA
XhoI 952 CTCGAG C^TCGAG
XmaCI 476 CCCGGG C^CCGGG
XmaI 476 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 1297 CGCG CG^CG
1922 CGCG
AcyI 235 GGCGCC G[GA]^CG[CT]C
3658 GACGCC
AflIII 2703 ACACGT A^C[GA][CT]GT
4010 ACATGT
Asp700I 3926 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4338 GAAGAGTTTC
AvrII 4751 CCTAGG C^CTAGG
4977 CCTAGG
BalI 636 TGGCCA TGG^CCA
5363 TGGCCA
BbsI 2520 GAAGAC GAAGAC(2/6)
1738 GTCTTC
BglI 1682 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4935 GCCCTGATGGC
BlnI 4751 CCTAGG C^CTAGG
4977 CCTAGG
BpuAI 2520 GAAGAC GAAGAC(2/6)
1738 GTCTTC
BsaHI 235 GGCGCC G[GA]^CG[CT]C
3658 GACGCC
BstUI 1297 CGCG CG^CG
1922 CGCG
EcoNI 1081 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4972 CCTGACCTAGG
Hin1I 235 GGCGCC G[GA]^CG[CT]C
3658 GACGCC
HpaI 4138 GTTAAC GTT^AAC
5154 GTTAAC
MluNI 636 TGGCCA TGG^CCA
5363 TGGCCA
MscI 636 TGGCCA TGG^CCA
5363 TGGCCA
MvnI 1297 CGCG CG^CG
1922 CGCG
NheI 547 GCTAGC G^CTAGC
4784 GCTAGC
PpuMI 1076 GGGACCC [GA]G^G[AT]CC[CT]
1090 GGGACCT
PvuII 470 CAGCTG CAG^CTG
2416 CAGCTG
SapI 2059 GAAGAGC GCTCTTC(1/4)
2181 GCTCTTC
SpeI 4480 ACTAGT A^CTAGT
5305 ACTAGT
TaqI 953 TCGA T^CGA
1454 TCGA
TthHB8I 953 TCGA T^CGA
1454 TCGA
XcmI 741 CCAAATCCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5258 CCAGCACAGTACTGG
XmnI 3926 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4338 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII