##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AvrII BamHI BanII
BbeI BcnI BlnI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrFI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FauI FseI
Hin1I HindIII KasI KpnI KspI
MamI MluI MroI NaeI NarI
NciI NgoMIV NotI NruI NsiI
PacI PflMI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Alw44I 3182 GTGCAC G^TGCAC
AocI 4385 CCTGAGG CC^T[ACGT]AGG
ApaLI 3182 GTGCAC G^TGCAC
Asp700I 4074 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2582 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4994 CTCGAG C^[CT]CG[GA]G
BalI 4923 TGGCCA TGG^CCA
BbrPI 2449 CACGTG CAC^GTG
BbvCI 3612 GCTGAGG CCTCAGC(-5/-2)
BcgI 942 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1059 GGATAC GTATCC(6/5)
BclI 1409 TGATCA T^GATCA
BglI 2004 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 326 AGATCT A^GATCT
BlpI 1591 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1591 GCTGAGC GC^T[ACGT]AGC
BsaWI 65 ACCGGA [AT]^CCGG[AT]
BseRI 2027 CTCCTC GAGGAG(10/8)
BsoBI 4994 CTCGAG C^[CT]CG[GA]G
BsrBI 718 GAGCGG CCGCTC(-3/-3)
BssSI 5215 CTCGTG CACGAG(-5/-1)
Bst1107I 1813 GTATAC GTA^TAC
BstAPI 1114 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3383 GGTTACC G^GT[ACGT]ACC
BstPI 3383 GGTTACC G^GT[ACGT]ACC
BstZ17I 1813 GTATAC GTA^TAC
Bsu36I 4385 CCTGAGG CC^T[ACGT]AGG
CelII 1591 GCTGAGC GC^T[ACGT]AGC
DraIII 5257 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 4923 TGGCCA [CT]^GGCC[GA]
Eco81I 4385 CCTGAGG CC^T[ACGT]AGG
EcoNI 4930 CCTTTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3383 GGTTACC G^GT[ACGT]ACC
EcoRI 4471 GAATTC G^AATTC
EcoRV 3554 GATATC GAT^ATC
FbaI 1409 TGATCA T^GATCA
HaeII 2103 AGCGCC [GA]GCGC^[CT]
HapII 66 CCGG C^CGG
HpaI 616 GTTAAC GTT^AAC
HpaII 66 CCGG C^CGG
MfeI 1067 CAATTG C^AATTG
MluNI 4923 TGGCCA TGG^CCA
MscI 4923 TGGCCA TGG^CCA
MspI 66 CCGG C^CGG
MunI 1067 CAATTG C^AATTG
NheI 309 GCTAGC G^CTAGC
PaeR7I 4994 CTCGAG C^TCGAG
PflFI 2582 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2449 CACGTG CAC^GTG
PmlI 2449 CACGTG CAC^GTG
SalI 2303 GTCGAC G^TCGAC
SwaI 3503 ATTTAAAT ATTT^AAAT
Tth111I 2582 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2037 TCTAGA T^CTAGA
XhoI 4994 CTCGAG C^TCGAG
XmnI 4074 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 2455 CGCG CG^CG
2457 CGCG
AspHI 3182 GTGCAC G[AT]GC[AT]^C
3980 GTGCTC
AviII 1649 TGCGCA TGC^GCA
4541 TGCGCA
BanI 2051 GGTGCC G^G[CT][GA]CC
5223 GGTGCC
BmyI 3182 GTGCAC G[AGT]GC[ACT]^C
3980 GTGCTC
BsaAI 2449 CACGTG [CT]AC^GT[GA]
5134 CACGTA
BsgI 1202 GTGCAG GTGCAG(16/14)
3688 GTGCAG
BsiHKAI 3182 GTGCAC G[AT]GC[AT]^C
3980 GTGCTC
Bsp1286I 3182 GTGCAC G[AGT]GC[ACT]^C
3980 GTGCTC
Bsp1407I 2793 TGTACA T^GTACA
3714 TGTACA
BspMI 422 ACCTGC ACCTGC(4/8)
3339 GCAGGT
BsrGI 2793 TGTACA T^GTACA
3714 TGTACA
BssHII 2454 GCGCGC G^CGCGC
2456 GCGCGC
BstBI 710 TTCGAA TT^CGAA
1469 TTCGAA
BstUI 2455 CGCG CG^CG
2457 CGCG
FspI 1649 TGCGCA TGC^GCA
4541 TGCGCA
HgaI 2586 GCGTC GACGC(5/10)
4417 GCGTC
HincII 616 GTTAAC GT[CT]^[GA]AC
2303 GTCGAC
HindII 616 GTTAAC GT[CT]^[GA]AC
2303 GTCGAC
MvnI 2455 CGCG CG^CG
2457 CGCG
NspV 710 TTCGAA TT^CGAA
1469 TTCGAA
PpuMI 3632 AGGACCT [GA]G^G[AT]CC[CT]
5288 GGGACCT
PstI 181 CTGCAG CTGCA^G
2568 CTGCAG
SfuI 710 TTCGAA TT^CGAA
1469 TTCGAA
SspBI 2793 TGTACA T^GTACA
3714 TGTACA
StuI 1538 AGGCCT AGG^CCT
4622 AGGCCT
XcmI 362 CCATGAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1748 CCAACCCTTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AvaII BbsI BbvI
BfaI BfrI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
BspHI BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII