################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AvrII BamHI BanII BbeI BcnI BlnI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrFI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FauI FseI Hin1I HindIII KasI KpnI KspI MamI MluI MroI NaeI NarI NciI NgoMIV NotI NruI NsiI PacI PflMI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Alw44I 3182 GTGCAC G^TGCAC AocI 4385 CCTGAGG CC^T[ACGT]AGG ApaLI 3182 GTGCAC G^TGCAC Asp700I 4074 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2582 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 4994 CTCGAG C^[CT]CG[GA]G BalI 4923 TGGCCA TGG^CCA BbrPI 2449 CACGTG CAC^GTG BbvCI 3612 GCTGAGG CCTCAGC(-5/-2) BcgI 942 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1059 GGATAC GTATCC(6/5) BclI 1409 TGATCA T^GATCA BglI 2004 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 326 AGATCT A^GATCT BlpI 1591 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1591 GCTGAGC GC^T[ACGT]AGC BsaWI 65 ACCGGA [AT]^CCGG[AT] BseRI 2027 CTCCTC GAGGAG(10/8) BsoBI 4994 CTCGAG C^[CT]CG[GA]G BsrBI 718 GAGCGG CCGCTC(-3/-3) BssSI 5215 CTCGTG CACGAG(-5/-1) Bst1107I 1813 GTATAC GTA^TAC BstAPI 1114 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3383 GGTTACC G^GT[ACGT]ACC BstPI 3383 GGTTACC G^GT[ACGT]ACC BstZ17I 1813 GTATAC GTA^TAC Bsu36I 4385 CCTGAGG CC^T[ACGT]AGG CelII 1591 GCTGAGC GC^T[ACGT]AGC DraIII 5257 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 4923 TGGCCA [CT]^GGCC[GA] Eco81I 4385 CCTGAGG CC^T[ACGT]AGG EcoNI 4930 CCTTTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3383 GGTTACC G^GT[ACGT]ACC EcoRI 4471 GAATTC G^AATTC EcoRV 3554 GATATC GAT^ATC FbaI 1409 TGATCA T^GATCA HaeII 2103 AGCGCC [GA]GCGC^[CT] HapII 66 CCGG C^CGG HpaI 616 GTTAAC GTT^AAC HpaII 66 CCGG C^CGG MfeI 1067 CAATTG C^AATTG MluNI 4923 TGGCCA TGG^CCA MscI 4923 TGGCCA TGG^CCA MspI 66 CCGG C^CGG MunI 1067 CAATTG C^AATTG NheI 309 GCTAGC G^CTAGC PaeR7I 4994 CTCGAG C^TCGAG PflFI 2582 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2449 CACGTG CAC^GTG PmlI 2449 CACGTG CAC^GTG SalI 2303 GTCGAC G^TCGAC SwaI 3503 ATTTAAAT ATTT^AAAT Tth111I 2582 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2037 TCTAGA T^CTAGA XhoI 4994 CTCGAG C^TCGAG XmnI 4074 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 2455 CGCG CG^CG 2457 CGCG AspHI 3182 GTGCAC G[AT]GC[AT]^C 3980 GTGCTC AviII 1649 TGCGCA TGC^GCA 4541 TGCGCA BanI 2051 GGTGCC G^G[CT][GA]CC 5223 GGTGCC BmyI 3182 GTGCAC G[AGT]GC[ACT]^C 3980 GTGCTC BsaAI 2449 CACGTG [CT]AC^GT[GA] 5134 CACGTA BsgI 1202 GTGCAG GTGCAG(16/14) 3688 GTGCAG BsiHKAI 3182 GTGCAC G[AT]GC[AT]^C 3980 GTGCTC Bsp1286I 3182 GTGCAC G[AGT]GC[ACT]^C 3980 GTGCTC Bsp1407I 2793 TGTACA T^GTACA 3714 TGTACA BspMI 422 ACCTGC ACCTGC(4/8) 3339 GCAGGT BsrGI 2793 TGTACA T^GTACA 3714 TGTACA BssHII 2454 GCGCGC G^CGCGC 2456 GCGCGC BstBI 710 TTCGAA TT^CGAA 1469 TTCGAA BstUI 2455 CGCG CG^CG 2457 CGCG FspI 1649 TGCGCA TGC^GCA 4541 TGCGCA HgaI 2586 GCGTC GACGC(5/10) 4417 GCGTC HincII 616 GTTAAC GT[CT]^[GA]AC 2303 GTCGAC HindII 616 GTTAAC GT[CT]^[GA]AC 2303 GTCGAC MvnI 2455 CGCG CG^CG 2457 CGCG NspV 710 TTCGAA TT^CGAA 1469 TTCGAA PpuMI 3632 AGGACCT [GA]G^G[AT]CC[CT] 5288 GGGACCT PstI 181 CTGCAG CTGCA^G 2568 CTGCAG SfuI 710 TTCGAA TT^CGAA 1469 TTCGAA SspBI 2793 TGTACA T^GTACA 3714 TGTACA StuI 1538 AGGCCT AGG^CCT 4622 AGGCCT XcmI 362 CCATGAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1748 CCAACCCTTACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI AvaII BbsI BbvI BfaI BfrI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI BspHI BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII